ID D3KIA8_GIAIC Unreviewed; 544 AA.
AC D3KIA8;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000256|ARBA:ARBA00039242};
DE EC=2.5.1.25 {ECO:0000256|ARBA:ARBA00012386};
DE AltName: Full=DTW domain-containing protein 1 {ECO:0000256|ARBA:ARBA00042508};
GN ORFNames=GL50803_15898 {ECO:0000313|EMBL:EFD95663.1};
OS Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=184922 {ECO:0000313|EMBL:EFD95663.1};
RN [1] {ECO:0000313|EMBL:EFD95663.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WB C6 {ECO:0000313|EMBL:EFD95663.1};
RX PubMed=17901334; DOI=10.1126/science.1143837;
RA Morrison H.G., McArthur A.G., Gillin F.D., Aley S.B., Adam R.D.,
RA Olsen G.J., Best A.A., Cande W.Z., Chen F., Cipriano M.J., Davids B.J.,
RA Dawson S.C., Elmendorf H.G., Hehl A.B., Holder M.E., Huse S.M., Kim U.U.,
RA Lasek-Nesselquist E., Manning G., Nigam A., Nixon J.E., Palm D.,
RA Passamaneck N.E., Prabhu A., Reich C.I., Reiner D.S., Samuelson J.,
RA Svard S.G., Sogin M.L.;
RT "Genomic minimalism in the early diverging intestinal parasite Giardia
RT lamblia.";
RL Science 317:1921-1926(2007).
CC -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC (acp3U(20)). {ECO:0000256|ARBA:ARBA00037050}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00024168};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC {ECO:0000256|ARBA:ARBA00038290}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFD95663.1}.
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DR EMBL; AACB02000064; EFD95663.1; -; Genomic_DNA.
DR AlphaFoldDB; D3KIA8; -.
DR STRING; 184922.D3KIA8; -.
DR EnsemblProtists; EFD95663; EFD95663; GL50803_15898.
DR VEuPathDB; GiardiaDB:GL50803_15898; -.
DR HOGENOM; CLU_501044_0_0_1; -.
DR OMA; TIFWRQH; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR InterPro; IPR005636; DTW.
DR PANTHER; PTHR15627; NATURAL KILLER CELL-SPECIFIC ANTIGEN KLIP1; 1.
DR PANTHER; PTHR15627:SF8; TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 1; 1.
DR Pfam; PF03942; DTW; 1.
DR SMART; SM01144; DTW; 1.
PE 3: Inferred from homology;
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 34..231
FT /note="DTW"
FT /evidence="ECO:0000259|SMART:SM01144"
SQ SEQUENCE 544 AA; 60560 MW; 482D273C673DFDD0 CRC64;
MMSTILETIV PEVSPSTLLS IRTRAKCPKC GSMQQWYCYF CHLLTNAPSF SPARPPIPVS
FLCYRGEKNS KSSVHGVVTS SENVTVRYFS GEAEEDLRDT LENGSVLLMT GRGAKKVSEV
QWSAIRRIYI LDCTWNQVNK CLRQLGLYSP SSNITTASDA QKCTPSVQLV ELEPYTTIFW
RQHKQKNSGC LSSAEALYYL LSELNQQGLV TRNLDNILTF FLAQAVLIYK RSPWVNKQVD
AHYKEIGELF KRRWGIDVSV APQSSVQSGS VGCCPRCGSS AEGIIFKGSK GCVVCLREEL
KRALTKTLAR CPVFFPGGRF LVLSFSNLCF TAFRNYMRDY LGSSVSALDD VMLPYSLRQH
NDEGAGYFSI LHSALHHAQA ENIPLVFDIR DSNSVASTIM HSAVLGDLSP GIASAEEFLE
FPNLQVDPTI AYKRYLSDAT QLYKPKRQLG HVSLFRPFLS IPEHVLKCYA DPLLTNTNSS
KMLSLVPLSE EKQYLMPLLR ELVPSPEAVC GLSKKLEWRA QSGEVPCPLC GAALDDSKCI
CSQL
//
