ID D3PLE6_MEIRD Unreviewed; 1273 AA.
AC D3PLE6;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 27-MAR-2024, entry version 84.
DE RecName: Full=DNA polymerase I, thermostable {ECO:0000256|ARBA:ARBA00021109};
GN OrderedLocusNames=Mrub_2285 {ECO:0000313|EMBL:ADD29037.1};
GN ORFNames=K649_11105 {ECO:0000313|EMBL:AGK05513.1};
OS Meiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus
OS ruber).
OC Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Meiothermus.
OX NCBI_TaxID=504728 {ECO:0000313|EMBL:AGK05513.1, ECO:0000313|Proteomes:UP000013026};
RN [1] {ECO:0000313|EMBL:ADD29037.1, ECO:0000313|Proteomes:UP000006655}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35948 / DSM 1279 / VKM B-1258 / 21
RC {ECO:0000313|Proteomes:UP000006655}, and DSM 1279
RC {ECO:0000313|EMBL:ADD29037.1};
RX PubMed=21304689; DOI=10.4056/sigs.1032748;
RA Tindall B.J., Sikorski J., Lucas S., Goltsman E., Copeland A.,
RA Glavina Del Rio T., Nolan M., Tice H., Cheng J.F., Han C., Pitluck S.,
RA Liolios K., Ivanova N., Mavromatis K., Ovchinnikova G., Pati A.,
RA Fahnrich R., Goodwin L., Chen A., Palaniappan K., Land M., Hauser L.,
RA Chang Y.J., Jeffries C.D., Rohde M., Goker M., Woyke T., Bristow J.,
RA Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.,
RA Lapidus A.;
RT "Complete genome sequence of Meiothermus ruber type strain (21).";
RL Stand. Genomic Sci. 3:26-36(2010).
RN [2] {ECO:0000313|EMBL:AGK05513.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 1279 {ECO:0000313|EMBL:AGK05513.1};
RA Klammer A., Drake J., Heiner C., Clum A., Copeland A., Huddleston J.,
RA Eichler E., Turner S.W.;
RT "Non-Hybrid, Finished Microbial Genome Assemblies from Long-Read SMRT
RT Sequencing Data.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AGK05513.1, ECO:0000313|Proteomes:UP000013026}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35948 / DSM 1279 / VKM B-1258 / 21
RC {ECO:0000313|Proteomes:UP000013026}, and DSM 1279
RC {ECO:0000313|EMBL:AGK05513.1};
RA Chin J., Alexander D.H., Marks P., Korlach J., Clum A., Copeland A.;
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
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DR EMBL; CP001743; ADD29037.1; -; Genomic_DNA.
DR EMBL; CP005385; AGK05513.1; -; Genomic_DNA.
DR RefSeq; WP_013014535.1; NC_021081.1.
DR AlphaFoldDB; D3PLE6; -.
DR STRING; 504728.K649_11105; -.
DR KEGG; mrb:Mrub_2285; -.
DR KEGG; mre:K649_11105; -.
DR PATRIC; fig|504728.9.peg.2289; -.
DR eggNOG; COG0258; Bacteria.
DR eggNOG; COG0749; Bacteria.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000006655; Chromosome.
DR Proteomes; UP000013026; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0001882; F:nucleoside binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd00081; Hint; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 2.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 3.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR015361; Taq_pol_thermo_exonuc.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 2.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF09281; Taq-exonuc; 1.
DR PRINTS; PR00868; DNAPOLI.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 2.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:ADD29037.1};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ADD29037.1}.
FT DOMAIN 803..945
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 1084..1106
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
SQ SEQUENCE 1273 AA; 143362 MW; DA3A95CD8FBB60B1 CRC64;
MEQPSLFSSE SPSVELPRPD RIWLVDGHHL AYRSYFAFEK LATSRGEPTQ AIFGFLRTLL
KLLKEDGDCV IVVFDPPTRT FRHDAFEEYK AGRAATPDDF HPQLEKIKEL VDLMGLQRLE
VPGYEADDVI GTLAKKAEQE GYPVRILTGD RDSFQLLSEA VQVMLPDGRL MHPQAVQEKY
GVSVAQWVDY RSLVGDSSDN LPGAKGIGEK TAAKLLQEWG SLEGLYANLE ALSPKIRASL
EESRDNVQLS RTLSLIHTDL PLELDFRDCH RREPDRGALR EALEKLEFGS ILRELNLLAA
PPAAEEAPWP PPPEAFLGYT LDRAQPMWAN WLGLAAYAGG KVYRGQPSLQ ELKKFPEIRS
MVAKDLSVLA LKEGVWVPPG DDPMLLAYLY DPSNSDPAST VRRYGAGDWG SDPGERAQAA
HTLWETLQSR IGENPKIEWL YQQIEKPLSA VLARIEARGI SLNAEYLLQL SEELAKEISY
LEAEIHRLAG RSFNVNSRDQ LEVVLYDELK LSAPGRKTQT GKRSTAASAL EELLGQHPII
ERILTYRELT KLKSTYLDPL PKLIHPKTGR LHTRFNQTGT ATGRLSSVDP NLQNIPVRTE
IGRRIRKAFR AAPGMRLVVA DYSQIELRVL AHLSGDENLI NVFREGRDIH TQTAAWMFGL
EPDKVGAEQR RAAKCLVEGT KVATGRGWLP IEQVRVGDCV QTDGKMRRVV GLLDNGIQPT
IRISTQRGFE LRGTPHHRIR VVAKDGAVVW RQLQHLQAGD QVVLRRHGAF GYCNDLPPLE
PVAITRHKPL PWPQHLDQKM ALWLGYMVAE GTAIRAKQHR ERYGVVLMGL GRRDLDVLDE
VGALWEWLAG SRLKRYEKQR SVHWAVASTD LAEWIAKWCG AKSDQKRIPN FLWQAPASIQ
ASFLQALFEG DGSISRRRGT TYIRFATASL QLAKDVQRML LNFGIASTRS AEPRGQHKTS
HILLIGEPSD VERFAKHIGF RSQRKSALLR QHTTRRSTYI MPHQEQRLRR IYAALSLKGR
DKEKIYECIR THAQPVGLNA TRIQLLLSDP AISGHRYTRV WRWWHENDLF ADAISLVEVA
KPARVYDLSV EGKPAYLAEG FVSHNTINFG VLYGMSAHRL SNELSISYAE AEGFIERYFA
SYPKVREWIE RVLADARQTG YVETMFGRRR FVADLGSRVR SVREAAERMA FNMPVQGTAA
DLMKLAMVKL QPELEPLEAH LVLQVHDELL VEAPADKAEA VAGVVREVMQ QAWALAVPLE
VGTGIGENWL EAK
//
