ID D3YXE5_MOUSE Unreviewed; 458 AA.
AC D3YXE5;
DT 20-APR-2010, integrated into UniProtKB/TrEMBL.
DT 20-APR-2010, sequence version 1.
DT 27-MAR-2024, entry version 94.
DE RecName: Full=Sulfotransferase {ECO:0000256|RuleBase:RU361155};
DE EC=2.8.2.- {ECO:0000256|RuleBase:RU361155};
GN Name=Ndst3 {ECO:0000313|Ensembl:ENSMUSP00000120623.2,
GN ECO:0000313|MGI:MGI:1932544};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000120623.2, ECO:0000313|Proteomes:UP000000589};
RN [1] {ECO:0000313|Ensembl:ENSMUSP00000120623.2, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000120623.2,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000313|Ensembl:ENSMUSP00000120623.2}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000120623.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005093}.
CC -!- PATHWAY: Glycan metabolism; heparin biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004841}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}.
CC -!- SIMILARITY: Belongs to the sulfotransferase 1 family. NDST subfamily.
CC {ECO:0000256|ARBA:ARBA00010420}.
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DR RefSeq; NP_001280611.1; NM_001293682.1.
DR RefSeq; XP_006502439.1; XM_006502376.3.
DR ProteomicsDB; 332458; -.
DR Antibodypedia; 26593; 146 antibodies from 18 providers.
DR DNASU; 83398; -.
DR Ensembl; ENSMUST00000124803.8; ENSMUSP00000122617.2; ENSMUSG00000027977.16.
DR Ensembl; ENSMUST00000132112.8; ENSMUSP00000120623.2; ENSMUSG00000027977.16.
DR GeneID; 83398; -.
DR UCSC; uc008rfo.1; mouse.
DR AGR; MGI:1932544; -.
DR CTD; 9348; -.
DR MGI; MGI:1932544; Ndst3.
DR VEuPathDB; HostDB:ENSMUSG00000027977; -.
DR GeneTree; ENSGT00940000160665; -.
DR HOGENOM; CLU_011357_0_1_1; -.
DR OrthoDB; 2913264at2759; -.
DR UniPathway; UPA00756; -.
DR UniPathway; UPA00862; -.
DR BioGRID-ORCS; 83398; 2 hits in 76 CRISPR screens.
DR ChiTaRS; Ndst3; mouse.
DR Proteomes; UP000000589; Chromosome 3.
DR Bgee; ENSMUSG00000027977; Expressed in cerebellar cortex and 61 other cell types or tissues.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015016; F:[heparan sulfate]-glucosamine N-sulfotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019213; F:deacetylase activity; IEA:UniProt.
DR GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0030210; P:heparin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR021930; Heparan_SO4_deacetylase.
DR InterPro; IPR037359; NST/OST.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR PANTHER; PTHR10605:SF29; BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE 3; 1.
DR PANTHER; PTHR10605; HEPARAN SULFATE SULFOTRANSFERASE; 1.
DR Pfam; PF12062; HSNSD; 1.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR637359-3};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW Transferase {ECO:0000256|RuleBase:RU361155}.
FT DOMAIN 1..91
FT /note="Heparan sulphate-N-deacetylase"
FT /evidence="ECO:0000259|Pfam:PF12062"
FT DOMAIN 181..429
FT /note="Sulfotransferase"
FT /evidence="ECO:0000259|Pfam:PF00685"
FT ACT_SITE 190
FT /note="For sulfotransferase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-1"
FT BINDING 288
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 393
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 409..413
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT DISULFID 394..404
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-3"
SQ SEQUENCE 458 AA; 53495 MW; 628B9D11DEF24DA4 CRC64;
MGYAVSPHHS GVYPVHVQLY EAWKKVWNIK ITSTEEYPHL KPARYRRGFI HKNIMVLPRQ
TCGLFTHTIF YKEYPGGPRE LDKSIHGGEL FFTVVLNPIS IFMTHLSNYG NDRLGLYTFV
NLANFVQTWT NLRLQTLPPA QLAHKYFELF PDQKDPLWQN PCDDKRHRDI WSKEKTCDRL
PKFLVIGPQK TGTTALCLFL IMHPSILSNS PSPKSFEEVQ FFNRNNYHRG IDWYMDFFPV
PSNVTTDFLF EKSANYFHSE DAPKRAASLV PKAKIITILI DPSDRAYSWY QHQRSHEDPA
ALKFSFYEVI SAGPNAPWEL RTLQKRCLVP GWYANHIERW LVYFPPFQLL IIDGQQLRTT
PATVMDEVQK FLGVSPHYNY SEALTFDSHK GFWCQLLEEG KTKCLGKSKG RKYPPMDSDS
RAFLSSYYRD HNVELSKLLH RLGQPLPSWL RQELQKVR
//
