ID D4BMY8_BIFBR Unreviewed; 691 AA.
AC D4BMY8;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 24-JAN-2024, entry version 66.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=BIFBRE_03435 {ECO:0000313|EMBL:EFE89025.1};
OS Bifidobacterium breve DSM 20213 = JCM 1192.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=518634 {ECO:0000313|EMBL:EFE89025.1, ECO:0000313|Proteomes:UP000003191};
RN [1] {ECO:0000313|EMBL:EFE89025.1, ECO:0000313|Proteomes:UP000003191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20213 {ECO:0000313|EMBL:EFE89025.1,
RC ECO:0000313|Proteomes:UP000003191};
RA Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B., Courtney L.,
RA Fronick C., Harrison M., Strong C., Farmer C., Delahaunty K., Markovic C.,
RA Hall O., Minx P., Tomlinson C., Mitreva M., Nelson J., Hou S., Wollam A.,
RA Pepin K.H., Johnson M., Bhonagiri V., Zhang X., Suruliraj S., Warren W.,
RA Chinwalla A., Mardis E.R., Wilson R.K.;
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFE89025.1}.
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DR EMBL; ACCG02000009; EFE89025.1; -; Genomic_DNA.
DR RefSeq; WP_003828493.1; NZ_JDUD01000004.1.
DR AlphaFoldDB; D4BMY8; -.
DR SMR; D4BMY8; -.
DR STRING; 1685.RY69_1497; -.
DR KEGG; bbrd:BBBR_0453; -.
DR PATRIC; fig|518634.20.peg.478; -.
DR HOGENOM; CLU_012430_1_1_11; -.
DR Proteomes; UP000003191; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013739; Beta_galactosidase_C.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08533; Glyco_hydro_42C; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000003191}.
FT DOMAIN 24..396
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 410..620
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 632..690
FT /note="Beta-galactosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08533"
FT ACT_SITE 160
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 318
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 121
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 159
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 326
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 691 AA; 77346 MW; 7A998DCDC816284A CRC64;
MEHREFKWPQ PLAGGKPRIW YGGDYNPDQW PEEVWDEDVA LMQKAGVNLV SVAIFSWAKL
EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT QAHPEILWVD YRGDVCQPGA
RQHWRATSPV FLDYALNLCR KMAEHYKDNP YVVSWHVSNE YGCHNRFDYS EDAERAFQKW
CEKKYGTIDA VNDAWGTAFW AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD
FYKAERDALL EIAPKPQTTN FMVSAGGAGI DYDKWGYDVD FVSNDHYFTP GEAHFDELAY
SASLCDGIAR KNPWFLMEHS SSAVNWRPIN YRVEPGELVR DSLAHLAMGS DAICYFQWRQ
SKAGAEKWHS SMVPHAGPDS QIFRDVCELG ADLNKLADEG LLSTKLVKSK VAVVFDYESQ
WATEHTATPT QEVRHWTEPL AWFRALADNG LTADVVPVRG PWDEYEAVVL PSLTILSEET
TRRVREYVAN GGKLFVTYYT GLVDDKDHVW LGGYPGSIRD VVGVRVEEFA PMGNDFPGAI
DHLDLDNGTV AHDFADVITS VADTAHVVAA FKADKWTGFD GAPAITVNDF GDGKAAYIGA
RLGREGLAKS LPALLEELGI ETSAEDDRGE VLRVERADET GENHFVFLFN RTHDVAIVDV
DGEPLVASLA QVNESEHTAA IQPNGVLVVK L
//
