ID D4E018_SEROD Unreviewed; 416 AA.
AC D4E018;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=Lipid III flippase {ECO:0000256|HAMAP-Rule:MF_02024};
GN Name=wzxE {ECO:0000256|HAMAP-Rule:MF_02024,
GN ECO:0000313|EMBL:EFE96924.1};
GN ORFNames=HMPREF0758_1518 {ECO:0000313|EMBL:EFE96924.1};
OS Serratia odorifera DSM 4582.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=667129 {ECO:0000313|EMBL:EFE96924.1, ECO:0000313|Proteomes:UP000005723};
RN [1] {ECO:0000313|EMBL:EFE96924.1, ECO:0000313|Proteomes:UP000005723}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 4582 {ECO:0000313|EMBL:EFE96924.1,
RC ECO:0000313|Proteomes:UP000005723};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates the transbilayer movement of Und-PP-GlcNAc-ManNAcA-
CC Fuc4NAc (lipid III) from the inner to the outer leaflet of the
CC cytoplasmic membrane during the assembly of enterobacterial common
CC antigen (ECA). {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_02024}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_02024}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the polysaccharide transport (PST) (TC 2.A.66.2)
CC family. {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFE96924.1}.
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DR EMBL; ADBY01000025; EFE96924.1; -; Genomic_DNA.
DR RefSeq; WP_004957335.1; NZ_GG753567.1.
DR AlphaFoldDB; D4E018; -.
DR STRING; 667129.HMPREF0758_1518; -.
DR HOGENOM; CLU_042154_0_0_6; -.
DR OrthoDB; 9769862at2; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000005723; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13125; MATE_like_10; 1.
DR HAMAP; MF_02024; WzxE; 1.
DR InterPro; IPR002797; Polysacc_synth.
DR InterPro; IPR044550; WzxE.
DR InterPro; IPR032896; WzxE_Proteobacteria.
DR PANTHER; PTHR30250:SF30; LIPID III FLIPPASE; 1.
DR PANTHER; PTHR30250; PST FAMILY PREDICTED COLANIC ACID TRANSPORTER; 1.
DR Pfam; PF01943; Polysacc_synt; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_02024};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, ECO:0000256|HAMAP-
KW Rule:MF_02024};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_02024};
KW Reference proteome {ECO:0000313|Proteomes:UP000005723};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_02024};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_02024}; Transport {ECO:0000256|HAMAP-Rule:MF_02024}.
FT TRANSMEM 12..33
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 45..67
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 79..101
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 121..139
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 151..170
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 176..195
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 216..238
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 263..281
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 293..314
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 334..355
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 394..412
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
SQ SEQUENCE 416 AA; 44994 MW; 157F2BD861F83353 CRC64;
MSLAKASVWT AGSTLIKIGV GLLVVKLLAV AFGPSGVGQA GNFRQLITVL GVLSGAGIFN
GVTKYVAEFH QDPQRLRMAV GTASSIVLGF STLLALVFLF AAQPISIGLF GHAQYVNVIR
AVAFIQMGIA YANLFLAILK GYRDAMGNAA AVIGGSLIGV VAFVICFKLG GYEGALAGLA
LVPALVVLPA GILLWRRDNL PLRYLALAWD KALASHLGKF TIMALITSVT LPVAYVMMRN
LLAAHYSWDE VGIWQGVSSI SDAYLQFITA SFTVYLLPTL SRLSDKGAIT REIVRSLTFV
LPAVAAASFA VWLLRDFAIW LLFSDKFIAM RDLFAWQLVG DVLKVGAYVF GYLVIAKASL
RFYLLAEVSQ FVLLTAFAHW LIPLHGALGA AQAYMATYIV YFTLCSLIFI LYRRRV
//
