ID   D4M4H2_9FIRM            Unreviewed;       695 AA.
AC   D4M4H2;
DT   18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT   18-MAY-2010, sequence version 1.
DT   27-MAR-2024, entry version 68.
DE   SubName: Full=Isoamylase {ECO:0000313|EMBL:CBL26134.1};
DE            EC=3.2.1.- {ECO:0000313|EMBL:CBL26134.1};
GN   ORFNames=RTO_15340 {ECO:0000313|EMBL:CBL26134.1};
OS   [Ruminococcus] torques L2-14.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC   Mediterraneibacter.
OX   NCBI_TaxID=657313 {ECO:0000313|EMBL:CBL26134.1, ECO:0000313|Proteomes:UP000008956};
RN   [1] {ECO:0000313|EMBL:CBL26134.1, ECO:0000313|Proteomes:UP000008956}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L2-14 {ECO:0000313|EMBL:CBL26134.1,
RC   ECO:0000313|Proteomes:UP000008956};
RG   metaHIT consortium -- http://www.metahit.eu/;
RA   Pajon A., Turner K., Parkhill J., Duncan S., Flint H.;
RT   "The genome sequence of Ruminococcus torques L2-14.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBL26134.1, ECO:0000313|Proteomes:UP000008956}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L2-14 {ECO:0000313|EMBL:CBL26134.1,
RC   ECO:0000313|Proteomes:UP000008956};
RA   Pajon A.;
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
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DR   EMBL; FP929055; CBL26134.1; -; Genomic_DNA.
DR   AlphaFoldDB; D4M4H2; -.
DR   STRING; 33039.ERS852502_00061; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; rto:RTO_15340; -.
DR   PATRIC; fig|657313.3.peg.1334; -.
DR   HOGENOM; CLU_011725_2_1_9; -.
DR   Proteomes; UP000008956; Chromosome.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd11234; E_set_GDE_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000313|EMBL:CBL26134.1};
KW   Hydrolase {ECO:0000313|EMBL:CBL26134.1}.
FT   DOMAIN          164..574
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   695 AA;  79483 MW;  1795B491327390E5 CRC64;
     MPVISEQLKP IDTVNGFQIR PGFFREFGAV AIPGGVNFTI HTHGATSCEL LLFHRKAEEP
     YAVIPFPESY RIGFCYSMIV FDLDIEEFEY AYRLDGPYDE KKGLRFDKNK ILLDPYARAV
     TGQSQWGHVN NAQHGYRARV VQSNFDWGDQ RHHSIPMEDL IIYELHVRGF TMDESSGVKH
     HGTFEGLREK IPYLKELGVN AVELMPIFEF DEMRDVRLID ENELIDFWGY NPVSFFAPNT
     SYCSSMEYNR EGLELKTLIK DLHDNGIEVI LDVVFNHTAE GNEFGPCFSF KGFDNNIYYM
     LTPDGHYYNF SGCGNTLNCN HPVVRDMILE CLRYWVIEYR VDGFRFDLAS ILGRNDDGTP
     LSQPPLLRSL AFDSILGNVK LIAEAWDAGG LYQVGSFPSW KRWAEWNGRY RDDMRRFLKG
     DDFLAQTAAA RITGSPDLYD PAYRGGNASI NFLTCHDGFT LYDLYSYNQK HNEANGWGNT
     DGADDNNSWN CGVEGETDDP AILALRKRLM KNACAILLCS RGTPMFLSGD EFADTRYGNN
     NPYCQDNLIS WLDWSLLKKN KDLFDFFQYM IRFRKDHPVI RKDLEPSYLG IPAMSTHGLT
     PDETNFSGDS HVVCVRFAGY NESTQKEDLV YLAVNSGWFP VTLTLPELPE HYKWKVAVNT
     GDPKCQFFHK NSMPTVESKI FLGERSVIIF VATAI
//