ID D5GAW0_TUBMM Unreviewed; 1075 AA.
AC D5GAW0;
DT 15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT 15-JUN-2010, sequence version 1.
DT 13-SEP-2023, entry version 72.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=GSTUM_00005330001 {ECO:0000313|EMBL:CAZ81653.1};
OS Tuber melanosporum (strain Mel28) (Perigord black truffle).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Tuberaceae; Tuber.
OX NCBI_TaxID=656061 {ECO:0000313|EMBL:CAZ81653.1, ECO:0000313|Proteomes:UP000006911};
RN [1] {ECO:0000313|EMBL:CAZ81653.1, ECO:0000313|Proteomes:UP000006911}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mel28 {ECO:0000313|EMBL:CAZ81653.1,
RC ECO:0000313|Proteomes:UP000006911};
RX PubMed=20348908; DOI=10.1038/nature08867;
RA Martin F., Kohler A., Murat C., Balestrini R., Coutinho P.M., Jaillon O.,
RA Montanini B., Morin E., Noel B., Percudani R., Porcel B., Rubini A.,
RA Amicucci A., Amselem J., Anthouard V., Arcioni S., Artiguenave F.,
RA Aury J.M., Ballario P., Bolchi A., Brenna A., Brun A., Buee M.,
RA Cantarel B., Chevalier G., Couloux A., Da Silva C., Denoeud F.,
RA Duplessis S., Ghignone S., Hilselberger B., Iotti M., Marcais B., Mello A.,
RA Miranda M., Pacioni G., Quesneville H., Riccioni C., Ruotolo R.,
RA Splivallo R., Stocchi V., Tisserant E., Viscomi A.R., Zambonelli A.,
RA Zampieri E., Henrissat B., Lebrun M.H., Paolocci F., Bonfante P.,
RA Ottonello S., Wincker P.;
RT "Perigord black truffle genome uncovers evolutionary origins and mechanisms
RT of symbiosis.";
RL Nature 464:1033-1038(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; FN430086; CAZ81653.1; -; Genomic_DNA.
DR RefSeq; XP_002837462.1; XM_002837416.1.
DR AlphaFoldDB; D5GAW0; -.
DR STRING; 656061.D5GAW0; -.
DR EnsemblFungi; CAZ81653; CAZ81653; GSTUM_00005330001.
DR GeneID; 9187821; -.
DR KEGG; tml:GSTUM_00005330001; -.
DR eggNOG; KOG0434; Eukaryota.
DR HOGENOM; CLU_001493_1_1_1; -.
DR InParanoid; D5GAW0; -.
DR OMA; EIIVIHK; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000006911; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:EnsemblFungi.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000006911}.
FT DOMAIN 10..634
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 689..844
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1075 AA; 124235 MW; C3690946125C2F1C CRC64;
MDFPKEEERI LEFWREIDAF RTSVKLSEGR PPYSFYDGPP FCTGLPHYGH LLASTIKDIV
PRYWHMKGHY VERRFGWDTH GLPIEHEIDK RLGITSKEDV LKMGMEKYNE ECKAIVMRYS
TEWRQTIDRL GRWIDFDNDY KSMYPTFMES IWWVCKQLFV KDQVYLGYRV MPYSTACTTP
LSNFEAQQNY KDVQDPAVVV TFPLLDDPTT ELLAWTTTPW TLPSHTGIAV HPDFEYIKIS
DRASGRNYIL LESCLKTLYG EKGLKKAKYT ILEKIKGKDM LGWKYEPLFD YFYEQFKDYG
FKVLNATYVT SDSGTGLVHQ APAFGEEDYS VAMEAGVINE TRPPPNPVNE AGIFTSEVRE
FAGLHVKAAD KGIIKHLKEK KRLIVDSQIT HSYPFCWRSD TPLIYRAVPA WFVRIPNIIP
KMLENIEGSH WVPTNVKENR FGNWIQNARD WNISRNRYWG TPIPIWMSDD KEEMVCIGSV
EELRELSGCG EIPDLHRDKI DHITIPSKKG KGVLRRVEEV FDCWFESGSM PYASSHYPFE
NKDQFEKAFP ADFIAEGLDQ TRGWFYTLLV LGTHLFGVSP FQNCIVNGIV LAEDGKKMSK
RLKNYPDPTL VMDKYGSDAL RLYLINSPVV RAMPLRFKEV GVKDVVTKVL LPLWNSYNFF
EQQSQLLKKL EGVDFIFNPE SGGSKNVMDR WILANCQSYL KFVNQEMKGY RLYTVVGRML
EMVDNTTNWY IRFNRKRLKG EYGTEDTLHA LNSLFEVLFT LVRGMAPFAP FLTDNIYHRL
KPHIPDSMLP EDARSVHFLS FPEVREELFD EEVERRFGRM QRVIELARTS RERKAIGLKT
PLKTLVVIHP DQTYLDDVKS LESYITSELN VRDLVLSADE EKYGVQYKVT ADWPVLGKKL
KKDLIKVKNA LPSITSAEVK GFVQEKKIVV GGITLIEEDL VVSRGLSGSD TSQDFETNTD
NDVLTILDTV LHKDLRNEGL AREIVNRVQR LRKKAGLLPT DDIKMEYKVL QDPIGLEEVF
LEHADTFEKA LRRPLDKATI TQVGEVQPGE NLDKMILEET QEVGEATFML RLLKL
//
