ID D5UX42_TSUPD Unreviewed; 4264 AA.
AC D5UX42;
DT 13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT 13-JUL-2010, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE SubName: Full=Mycocerosate synthase., 6-deoxyerythronolide-B synthase {ECO:0000313|EMBL:ADG80061.1};
DE EC=2.3.1.111 {ECO:0000313|EMBL:ADG80061.1};
DE EC=2.3.1.94 {ECO:0000313|EMBL:ADG80061.1};
GN OrderedLocusNames=Tpau_3480 {ECO:0000313|EMBL:ADG80061.1};
OS Tsukamurella paurometabola (strain ATCC 8368 / DSM 20162 / CCUG 35730 / CIP
OS 100753 / JCM 10117 / KCTC 9821 / NBRC 16120 / NCIMB 702349 / NCTC 13040)
OS (Corynebacterium paurometabolum).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Tsukamurellaceae;
OC Tsukamurella.
OX NCBI_TaxID=521096 {ECO:0000313|EMBL:ADG80061.1, ECO:0000313|Proteomes:UP000001213};
RN [1] {ECO:0000313|Proteomes:UP000001213}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8368 / DSM 20162 / CCUG 35730 / CIP 100753 / JCM 10117 /
RC KCTC 9821 / NBRC 16120 / NCIMB 702349 / NCTC 13040
RC {ECO:0000313|Proteomes:UP000001213};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA Mikhailova N., Munk A.C., Brettin T., Detter J.C., Tapia R., Han C.,
RA Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P.,
RA Woyke T., Wu D., Jando M., Brambilla E., Klenk H.-P., Eisen J.A.;
RT "The complete chromosome of Tsukamurella paurometabola DSM 20162.";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADG80061.1, ECO:0000313|Proteomes:UP000001213}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8368 / DSM 20162 / CCUG 35730 / CIP 100753 / JCM 10117 /
RC KCTC 9821 / NBRC 16120 / NCIMB 702349 / NCTC 13040
RC {ECO:0000313|Proteomes:UP000001213};
RX PubMed=21886861;
RA Munk A.C., Lapidus A., Lucas S., Nolan M., Tice H., Cheng J.F.,
RA Del Rio T.G., Goodwin L., Pitluck S., Liolios K., Huntemann M., Ivanova N.,
RA Mavromatis K., Mikhailova N., Pati A., Chen A., Palaniappan K., Tapia R.,
RA Han C., Land M., Hauser L., Chang Y.J., Jeffries C.D., Brettin T.,
RA Yasawong M., Brambilla E.M., Rohde M., Sikorski J., Goker M., Detter J.C.,
RA Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Tsukamurella paurometabola type strain (no.
RT 33).";
RL Stand. Genomic Sci. 4:342-351(2011).
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DR EMBL; CP001966; ADG80061.1; -; Genomic_DNA.
DR STRING; 521096.Tpau_3480; -.
DR KEGG; tpr:Tpau_3480; -.
DR eggNOG; COG0300; Bacteria.
DR eggNOG; COG1028; Bacteria.
DR eggNOG; COG3321; Bacteria.
DR HOGENOM; CLU_000022_35_8_11; -.
DR Proteomes; UP000001213; Chromosome.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0047879; F:erythronolide synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0050111; F:mycocerosate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd08952; KR_1_SDR_x; 1.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Acyltransferase {ECO:0000313|EMBL:ADG80061.1};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000001213};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ADG80061.1}.
FT DOMAIN 15..429
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 936..1013
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1029..1456
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2655..2730
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2757..3180
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4114..4189
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 906..941
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2224..2247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2732..2756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4264 AA; 442037 MW; 05D2D3E3716F09BB CRC64;
MVGGEPGVGV AMVTTEKVAV VGISCTFPGG DSPSSWWSFL ETGAEGVADA ETLDAFDAWR
LGAPRGGLID AVTDFDAAFF RISPREARAM DPQQRILLQS SWKLLEDARI TDADRRANRI
AVYVGAMSSD YSALAARSGR VTDRHTLTST ARSLLANRLS HALGLGGESL TVDTGQSSSL
AAVHLAASAV ARGDTDIALA GGVNLILDPQ GTETAGAFGA LSPDGRCFTM DERANGYVRG
EGVGLIALKS LSRARADGDR IYCVIDGSAM RSDGADDALT VPARAGQART FRAAYENAGV
DPATIEYVEL HGTGTAVGDP IEAHALGEVL GTPRRAAGRD PLRVGSVKTV IGHLEGAAGI
AGFIRTALAI HHGVLPRSAN FVTPNPAAAL HENHLAVQVT PEIWAGPGRR AAVSAFGMGG
TNVHVVLSAV PAQAEEAAAG ADDGSGGAPS APDATVVPLL LSGPDDAALA RQARTWHDWL
SGRSDRERSD AARISLLRPR LGRTAVIAEP STAGCTAELA ALADSIAGPS TSVLGQATGD
GAGAGSVSWV FSGQGTQRAG AGAGLYSQHP VFAKTVDRIT EAFIPLLGVD LRRYLLAEPD
SPLSAELTDT RIAQAAVFTV QSGLAALLGA YGARPDSVTG HSVGEYAAAV VAGVMSEADA
VYLVAHRGLL MSALPSGGGM VSLRADADAV AELLGAGDTP VEIAAVNAAD NIVLSGESDA
IAEVIAAAAT RGISAKPLRV SHAFHSRAMD PVLAPFREHA TAVTLAEPTI PFVSTVSGRA
MTTELADPEY WVQQIRQPVA FHAAVTALAQ AGTRGFIEIG PQSSLTAVIG ETVPDATAVI
PVLHHSKSEQ QTLSTALGVH AVTTGTGRRT YELLRVHGQP LVEPPTYEFD RRRYWAFDAE
TSSAETDPGA AVFVGSPAPD AHARPEPTAD HASSDDPDAV PPKTVRAALA AVLGADAGEL
DLTATFTALG LDSMGAVELV QRLQPFTSTR LTPSVVFDHP TPASLIAYLG STDGAAETST
AAIASTAADD PVVVIAMDCL LPGGADGPAA LWRLLIDEVD AIGPFPDDRD WNTEGLFADD
PSVPGTSYAR EGGFLEAATS FDPAFFGLSP REATAMDPQQ RLLLQLTHRA WERAGLTEAD
LRGSDTGVFV GAMAQPYGPP MDSAAPETEG YLLTGVSPSV LSGRIAYHYG LTGPALTVDT
ACSSSLVAVH LAAQAIRSGA CSLALAGGVT VMPTPGMFVE LSRQQALSPS ARCRAFSADA
DGTGWGEGAG LVLLTRASIA RTRGIPVLAV IEGSAINQDG ASNGLTAPSG TAQQRVIDEA
TRAAGVDLAS VDAVEAHGTG TKLGDPIEAN ALIATYGQRP DTADPLWLGS LKSNVGHSQA
AAGVVGLIKM VLALGAERLP RTLHVTAPSP FVDWSAGRVE LLTEPRPWPV TPGRTRRAGI
SSFGISGTNA HLILAEPTWL EADDTAATAL PAPANPGADR LHLLAVTAPD AAALSRRCTD
LADFLEGTAT DLGDLAAVLA TTRTHWAHRV VVLAADTPTA VAALRAAATG DPFSGCRGIA
APGETTFVFP GQGAQWPGMA AGLIASEPVF EAELRRADAV IAELAGWSVI DVLTGAPGAP
DPERIDVIQP VLFATMVSLG ALWRSYGVRP DTVIGHSQGE VAAAYFAGAL SLRDAATVIV
VRSRVSRTAD GTGGMLSILA PRTEVATLLR ESGAALDIAA VNSSRSTTVA GLARDLDTFA
ETVAAAGFDC RRIPGVTMAG HSRVCEPLRE DLLAGLQAIR PRPASVEMLS TVTAAEVDGA
ALDADYWYRN LRETVDFHGA SLARLAQGRS VFIEVSTHPL LVNALSTTIE ESSAAGAGVG
TLSRGAGDRA TFLLALAQAF VSGVPADLRT RDGDGDPLGF RDRGLPARLP AYPFARTRLW
LPATPAGAVD AAALGGSDVA GPLVTARLHT PGAGGEVFTG RIAVDDPAAA VGLLVELALE
AGDEAGYALL ARLDVTGPLP PARECIAQVH LAEVGGERTA FTIHVRSTGG EDDWVQVAEG
ELRDSPAVAT VPDPLPASAT VDPELVTGTV HTGGAGPDES PVSIAAVRRG PEALDVDLDL
PDDSDHPDSA AAILAPALLG AAGTLAGHLL DPDGAAAPLA VLRVEGLGSL LQGVRAVRIA
LRMRGGTESG AVVDMTITDR TGAPVTPTAT LTLGTPRTMA ASAIEARRTM YVVDWRDAAP
HDVAVGTGQE RGPEPYLVPD GTDATAPQRA REVTETVLER LREHLAADDP EAVLAIHTRH
AVVTGLEHEE ANPVHAAVWG LVRSAQTETP GRFVLIDSDG HPDSAAAVSA AGRAGHPQVA
VRAGRLCLPR LRRYSEHPSD SPRPANNGTV LVTGATGGLG RMVAEHLVRE HGVRDLVLVS
RGGPRSSGAD ELIANLIDLG ARPELVACDA ADRDAIAEVI AGITAHRPPL RAVIHTAGVL
DDATIANLTR EKIDAVFRAK VDALEHLDEL TADLDLDYFL VYSSMVASFG NPGQANYAAA
NAYMESLISR RRRRGLAGTA IGWGYWATDS AMSAHLSELD ISRIRRTGVI PLGADQGMAV
VDTLLAAPGQ DAPPAVLAVP FDPTVITSRA TDPELSPLIR SLAPPSRRRA VATRTTTGTA
LQRLLARALP AEWRRLTAEL VRTQVRTVLG TAPDSPLDGG RALRDIGFDS LTSVELRNRL
AAETGLALPT TLVFDHPTPD HLTDYLLRQV AEDEEAPDGV DDAAPDRPDA SPRSLDDDPI
VIVSMACRLP GGVRSADDLW NLVAHGRHGI GPFPEDRGWP AELYHADPAA KGHSYTRHGG
FIYDAAEFDA GFFGMSPREA LATDPQHRLM LETVWEALEE AAIDPHSLRG ANVGVYIGSV
GQDYVHLGDR VDESLEGFLV TGRASSVLSG RVSYTFGFEG PAITVDTACS SSLVALHLAV
TALRRDECEM AVVGGVSIMA DPGLFIEFSR QRGLAADGHC KTFSEQADGT AWAEGIGVLL
VERRSHALRY GHRILAEVSG TAINQDGASN GLTAPSGPAQ RRVIAAALAD AGVAPSEIDL
VEAHGTGTPL GDPIEAGALI EAYGEHRDGA APLYVGALKT NTGHTQGAAG IAGVIKTVLA
LRHQLMPRSL WGRNRSSAVT WQGVELLPDE RPWPHGERRR RAGVSAFGIS GTNAHVIVSE
PVVPSDTPHR APEGVAPEEL GLTVWPVSAD DPDALSVLLE SVPHALEHTA DPADAGAALT
TGRAQLTHRA VLLPGEPEPI ARGAVTDAGT VFVFSGQGGQ WLDMGRALAE ASPTFASALD
DVCAAVDRYL DRPIRDIMWH SDSGDSGTDA LLNDTRYSQP AIFAIQVALL RLVEALGLRP
DAVIGHSVGE VAAAHAAGIL GLDAAARFIA ARGTQMSAAP AGGAMAAIAA PPDRVHGLIT
RDASPHHGTD PVVIAAINGP AAVTVSGSAA AVAAAVDAAT AAGIRTKTLQ VSHAFHSAQM
AAAAHGLAGV ADEIDFQKPR IPFLPTGGRS RAVHNPAYWT EQVTAPVDFL GAVNEAAERG
PARYLVLGPD SALVAALREA TNGSVATALM SPKTGTTAQG AARDAATALG RLWADGAPVT
WAALYRDRDP HAVRMPSYPF QRKHYWIIPD RRSASAPPTG LCYESSWIPI PAAAGGAPVD
RVVLAVAADT HTDVVDTVRK ALLEAGHEPI VATVDEIERS TIVSGATTVL ALLSFTDSER
APEDTLRLAQ LAERSGAALV VVTGADGGAH TAVAALARTI GLEAPHTIST IVQIASPTDL
AQAGRLAGII GGSPEREIAV STESGIAGRR LVRVDSADTA PFRPSGTVLI TGGTGGLGSE
IARWCARNGA ERVVLASRGG ERSPGAASLV AELDELGTDC TVLTADCGDR PAFRSALSAF
EGASGGEITA VFHAAGIVDF TSFTATEPAD VRRLAAGKVT GARVLDEYFH DRELDAFVLF
SSIAATWGSA GQSAYSAANG YLDGLAAQRR ARGLAAISVA WGPWAEVGMI TTDGVADALD
KHGLIAMPAA AAIAALADTL GNPTVGAAVV IADVDWRRFL RTYSATGDTC LFELVANDHD
GESARPDDRS DHPRRTPQTL LDRLAQSPVE ARDAAVREVT DRLVAEVLGA ATHSRIDGTR
AFRDLGFDSL MTVELRDRLI AETGLAVSAM TIFDNPSPHE LHRHLLGLVS AALDPDPLSQ
LDQLETGLSA LAEDDARRTE LAARLRDLAD RFAEPRAAAD EPAPAELADA DADAVIAFLD
GLES
//
