ID D5XD13_THEPJ Unreviewed; 490 AA.
AC D5XD13;
DT 13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT 13-JUL-2010, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE SubName: Full=Cobalamin B12-binding domain protein {ECO:0000313|EMBL:ADG83689.1};
GN OrderedLocusNames=TherJR_2857 {ECO:0000313|EMBL:ADG83689.1};
OS Thermincola potens (strain JR).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Thermincolaceae;
OC Thermincola.
OX NCBI_TaxID=635013 {ECO:0000313|EMBL:ADG83689.1, ECO:0000313|Proteomes:UP000002377};
RN [1] {ECO:0000313|EMBL:ADG83689.1, ECO:0000313|Proteomes:UP000002377}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JR {ECO:0000313|EMBL:ADG83689.1,
RC ECO:0000313|Proteomes:UP000002377};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Hazen T.C., Woyke T.;
RT "Complete sequence of Thermincola sp. JR.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP002028; ADG83689.1; -; Genomic_DNA.
DR RefSeq; WP_013121679.1; NC_014152.1.
DR AlphaFoldDB; D5XD13; -.
DR STRING; 635013.TherJR_2857; -.
DR KEGG; tjr:TherJR_2857; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_021572_4_0_9; -.
DR Proteomes; UP000002377; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00302; anaerobic_magnesium-protoporph; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002377}.
FT DOMAIN 11..145
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 190..415
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 490 AA; 56181 MW; 1DBC9FA1FED5D303 CRC64;
MPKKLMLITP PYHAGVVEAA GSWPHLGFIY IAGHVRAAGF QAVIYDAMTK GHDLAQIKER
IREENPDFVG TTAYTSSICS AMDVLRAAKE VNPGIITLLG GIHANFCYRE LLEEQGDILD
IAVRGEGELT VPEIMEAFSG GKRLDGIPGV AYRLGNEVKI NPPRPFIGDL DSLIPAWDLV
EWEDYSFYVI PGSRLGIVNS SRGCINECSF CSQQKFWYRT YRERKAETFV EELEHLRDCY
GVNVVMLSDE YATRNRERWE RILDLMIERQ TGVYLLLETC VADIIRDADI LWKYRKAGVL
HIYVGVEATN QEKLDIFKKN VACENSREAI RLINEAGMIT ECSFVLGMPD ETPESIKSTL
KLAKHYNPDF AHFLLIAPWP YADIYQDLKD FVFEKDYAKY NFVEPVVKPK NMTVEEIHRA
VVDCYREYYM DKVKEYDRMK DPFKRDYLLR SMEVMMNNSF LVKHITGMGQ IPEEVRKYLA
GSRKQKCAGV
//
