ID D6GQS5_FILAD Unreviewed; 503 AA.
AC D6GQS5;
DT 13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT 13-JUL-2010, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=HMPREF0389_01050 {ECO:0000313|EMBL:EFE29128.1};
OS Filifactor alocis (strain ATCC 35896 / CCUG 47790 / D40 B5) (Fusobacterium
OS alocis).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC Filifactor.
OX NCBI_TaxID=546269 {ECO:0000313|EMBL:EFE29128.1, ECO:0000313|Proteomes:UP000007468};
RN [1] {ECO:0000313|Proteomes:UP000007468}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35896 / D40 B5 {ECO:0000313|Proteomes:UP000007468};
RG The Broad Institute Genome Sequencing Platform;
RA Ward D., Earl A., Feldgarden M., Young S.K., Gargeya S., Zeng Q.,
RA Alvarado L., Berlin A., Bochicchio J., Chapman S.B., Chen Z., Freedman E.,
RA Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D.,
RA Howarth C., Mehta T., Neiman D., Pearson M., Roberts A., Saif S., Shea T.,
RA Shenoy N., Sisk P., Stolte C., Sykes S., White J., Yandava C., Izard J.,
RA Blanton J.M., Baranova O.V., Tanner A.C., Dewhirst F.E., Haas B.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Filifactor alocis strain ATCC 35896.";
RL Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; CP002390; EFE29128.1; -; Genomic_DNA.
DR RefSeq; WP_014263036.1; NC_016630.1.
DR AlphaFoldDB; D6GQS5; -.
DR STRING; 546269.HMPREF0389_01050; -.
DR REBASE; 32320; M.Fal35896ORF1050P.
DR KEGG; faa:HMPREF0389_01050; -.
DR PATRIC; fig|546269.5.peg.1570; -.
DR eggNOG; COG0286; Bacteria.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000007468; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF4; TYPE I RESTRICTION ENZYME ECOKI METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EFE29128.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000007468};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..140
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 152..455
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 503 AA; 57962 MW; 76AAA01A477A6AC8 CRC64;
MGNQEIVQKL WNLCNVLRDD GITYHQYVTE LTYILFLKMM KEKDIEDTIE KNIVGYKDLK
KKNEDGTITK NEQQEFLEKK KVAEYSWNTL VSLSGIELKK YYERIIHLFG EHCRGHIKSI
YHNARTNIEE PKNLEKIIRT MNNLDWYSID EEGFGDLYEG LLEKNANEKK SGAGQYFTPR
VLIDVMTRLI QPKVGERCND PACGTFGFMI AAKRYVNEHH DEFSLSKEEY DFQKEKAFTG
CELVSDTHRL ALMNAMIHGI ESEILCADTL SNIGKSMSGY DVVLTNPPFG TKKGGERATR
DDFTFPTSNK QLNFLQHIYR SLKVDGKARA AVVLPDNVLF ADGDGAKIRE DLMDKCNLNM
ILRLPTGIFY AQGVKTNVLF FTRGTREKEN TKEVWFYDMR TNMPSFGKTT PLKKEHFVDF
EKAYLAEDRT KIEDERLNVF TREEIKVKNN SLDLGLIRDE NVLDYEDLQD PIESGEDMIG
QLEEALDLIK SVVKELKSLG SVN
//
