ID D6V3B5_9BRAD Unreviewed; 913 AA.
AC D6V3B5;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE SubName: Full=Nitrite transporter {ECO:0000313|EMBL:EFI52445.1};
GN ORFNames=AfiDRAFT_0431 {ECO:0000313|EMBL:EFI52445.1};
OS Afipia sp. 1NLS2.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Afipia.
OX NCBI_TaxID=666684 {ECO:0000313|EMBL:EFI52445.1, ECO:0000313|Proteomes:UP000004041};
RN [1] {ECO:0000313|EMBL:EFI52445.1, ECO:0000313|Proteomes:UP000004041}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1NLS2 {ECO:0000313|EMBL:EFI52445.1,
RC ECO:0000313|Proteomes:UP000004041};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Land M.L., Hauser L., Chang Y.-J., Jeffries C., Prakah O.,
RA Elkins J.G., Brown S.D., Palumbo A.V., Hemme C., Zhou J., Watson D.B.,
RA Jardine P.M., Kostka J.E., Green S.J., Woyke T.J.;
RT "The draft genome of Afipia sp. 1NLS2.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family.
CC {ECO:0000256|ARBA:ARBA00008432}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFI52445.1}.
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DR EMBL; ADVZ01000001; EFI52445.1; -; Genomic_DNA.
DR RefSeq; WP_009337377.1; NZ_ADVZ01000001.1.
DR AlphaFoldDB; D6V3B5; -.
DR eggNOG; COG2223; Bacteria.
DR Proteomes; UP000004041; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd17341; MFS_NRT2_like; 2.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 3.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515:SF2; HIGH AFFINITY NITRATE TRANSPORTER 2.5; 1.
DR PANTHER; PTHR23515; HIGH-AFFINITY NITRATE TRANSPORTER 2.3; 1.
DR Pfam; PF07690; MFS_1; 2.
DR SUPFAM; SSF103473; MFS general substrate transporter; 2.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 14..38
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 50..68
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 80..98
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 104..125
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 137..164
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 170..186
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 222..240
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 252..271
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 283..301
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 321..343
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 355..380
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 386..405
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 470..487
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 507..524
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 536..556
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 562..584
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 605..630
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 650..669
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 690..711
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 723..743
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 755..776
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 782..808
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 837..858
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 870..890
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 13..413
FT /note="Major facilitator superfamily (MFS) profile"
FT /evidence="ECO:0000259|PROSITE:PS50850"
SQ SEQUENCE 913 AA; 99383 MW; B88910C1277E4122 CRC64;
MSGPNQGNAA RGRALWLSTI SFTVCFAVWT IFSIIGISIK QKLGLNETEF GLLVGLPILS
GSLIRLVLGA WTDQYGGRRV NTIVMLAAAV ATYFLTWAHT YPQFLVAAFF VGIAGGSFAV
GVAYVSRFYP AGKQGTALGI FGAGNVGAAV TKLLAPFVMV AFGWQAVAEV WAAALAVMAV
IFWVFSEEDP VMRERRARGE KPKSMWLEFA PLKNIQVWRF SLYYFFVFGA FVALALWLPQ
YLINVYGVDL KAAGMIAAMF SIPASIFRAY GGHLADRYGA RRVMYWSLLV GVACTFILSY
PPTDYVVRTV TGSVGFHLEM GVVPFTVTVF VLGFFMALGK AAVFRHIPVY YPNNVGVVGG
LVGMIGGLGG FVLPIAFGAL NDLTGLWTSC FMLLFLIVTV SLLWMHLAVR QMERGLAGEA
LRKLPQFPEM EEIHKPEHIG ALAGVVLTDW RPEDREFWET KGRAIARRNL WISIPSLLLS
FAIWQVWSVV VAKLPSVGFK FTTAELFWLA ALPGISGAVL RIFYSFMVPI FGGRLWTTLA
TWSLMIPAVG IGYAVQSPST PYVVFLALAL LCGLGGGNFA SSMANISFFF PRAEKGNALA
LNAGLGNLGV SVVQFVVPLV ITAGVLGWFG GKPVMVADGP HQVPLWLQNA GFVWVPFIAA
SAFAAWFGMN DIASAKASFA DQAVIFQRKH NWVMCWLYTG TFGSFIGYSA GFPLLAKILF
PDVHVLPFVF LGPLVGALSR SGTGWIADRW GGGRVTLWVF ILMMAGVLGI LYFTGIREQP
GAFWGFFAMF LLMFFVTGVG NASTFQMIPN IMRKEMDRLM PEADAATRAR QTEKESAAIT
GFTSAIAAFG AFFIPKSFGT SIDMTGGVEA ALWGFFIFYV TCVAITWAVY TRKGGLLHDI
ERRGATAVAQ PAQ
//
