ID D6WLE2_TRICA Unreviewed; 1260 AA.
AC D6WLE2;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 2.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Sterol regulatory element-binding protein cleavage-activating protein {ECO:0000256|ARBA:ARBA00019541};
GN Name=AUGUSTUS-3.0.2_13456 {ECO:0000313|EMBL:EFA03461.2};
GN ORFNames=TcasGA2_TC013456 {ECO:0000313|EMBL:EFA03461.2};
OS Tribolium castaneum (Red flour beetle).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia;
OC Tenebrionidae; Tenebrionidae incertae sedis; Tribolium.
OX NCBI_TaxID=7070 {ECO:0000313|EMBL:EFA03461.2, ECO:0000313|Proteomes:UP000007266};
RN [1] {ECO:0000313|EMBL:EFA03461.2, ECO:0000313|Proteomes:UP000007266}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Georgia GA2 {ECO:0000313|EMBL:EFA03461.2,
RC ECO:0000313|Proteomes:UP000007266};
RX PubMed=18362917; DOI=10.1038/nature06784;
RG Tribolium Genome Sequencing Consortium;
RA Richards S., Gibbs R.A., Weinstock G.M., Brown S.J., Denell R.,
RA Beeman R.W., Gibbs R., Beeman R.W., Brown S.J., Bucher G., Friedrich M.,
RA Grimmelikhuijzen C.J., Klingler M., Lorenzen M., Richards S., Roth S.,
RA Schroder R., Tautz D., Zdobnov E.M., Muzny D., Gibbs R.A., Weinstock G.M.,
RA Attaway T., Bell S., Buhay C.J., Chandrabose M.N., Chavez D.,
RA Clerk-Blankenburg K.P., Cree A., Dao M., Davis C., Chacko J., Dinh H.,
RA Dugan-Rocha S., Fowler G., Garner T.T., Garnes J., Gnirke A., Hawes A.,
RA Hernandez J., Hines S., Holder M., Hume J., Jhangiani S.N., Joshi V.,
RA Khan Z.M., Jackson L., Kovar C., Kowis A., Lee S., Lewis L.R., Margolis J.,
RA Morgan M., Nazareth L.V., Nguyen N., Okwuonu G., Parker D., Richards S.,
RA Ruiz S.J., Santibanez J., Savard J., Scherer S.E., Schneider B.,
RA Sodergren E., Tautz D., Vattahil S., Villasana D., White C.S., Wright R.,
RA Park Y., Beeman R.W., Lord J., Oppert B., Lorenzen M., Brown S., Wang L.,
RA Savard J., Tautz D., Richards S., Weinstock G., Gibbs R.A., Liu Y.,
RA Worley K., Weinstock G., Elsik C.G., Reese J.T., Elhaik E., Landan G.,
RA Graur D., Arensburger P., Atkinson P., Beeman R.W., Beidler J., Brown S.J.,
RA Demuth J.P., Drury D.W., Du Y.Z., Fujiwara H., Lorenzen M., Maselli V.,
RA Osanai M., Park Y., Robertson H.M., Tu Z., Wang J.J., Wang S., Richards S.,
RA Song H., Zhang L., Sodergren E., Werner D., Stanke M., Morgenstern B.,
RA Solovyev V., Kosarev P., Brown G., Chen H.C., Ermolaeva O., Hlavina W.,
RA Kapustin Y., Kiryutin B., Kitts P., Maglott D., Pruitt K., Sapojnikov V.,
RA Souvorov A., Mackey A.J., Waterhouse R.M., Wyder S., Zdobnov E.M.,
RA Zdobnov E.M., Wyder S., Kriventseva E.V., Kadowaki T., Bork P., Aranda M.,
RA Bao R., Beermann A., Berns N., Bolognesi R., Bonneton F., Bopp D.,
RA Brown S.J., Bucher G., Butts T., Chaumot A., Denell R.E., Ferrier D.E.,
RA Friedrich M., Gordon C.M., Jindra M., Klingler M., Lan Q., Lattorff H.M.,
RA Laudet V., von Levetsow C., Liu Z., Lutz R., Lynch J.A., da Fonseca R.N.,
RA Posnien N., Reuter R., Roth S., Savard J., Schinko J.B., Schmitt C.,
RA Schoppmeier M., Schroder R., Shippy T.D., Simonnet F., Marques-Souza H.,
RA Tautz D., Tomoyasu Y., Trauner J., Van der Zee M., Vervoort M.,
RA Wittkopp N., Wimmer E.A., Yang X., Jones A.K., Sattelle D.B., Ebert P.R.,
RA Nelson D., Scott J.G., Beeman R.W., Muthukrishnan S., Kramer K.J.,
RA Arakane Y., Beeman R.W., Zhu Q., Hogenkamp D., Dixit R., Oppert B.,
RA Jiang H., Zou Z., Marshall J., Elpidina E., Vinokurov K., Oppert C.,
RA Zou Z., Evans J., Lu Z., Zhao P., Sumathipala N., Altincicek B.,
RA Vilcinskas A., Williams M., Hultmark D., Hetru C., Jiang H.,
RA Grimmelikhuijzen C.J., Hauser F., Cazzamali G., Williamson M., Park Y.,
RA Li B., Tanaka Y., Predel R., Neupert S., Schachtner J., Verleyen P.,
RA Raible F., Bork P., Friedrich M., Walden K.K., Robertson H.M., Angeli S.,
RA Foret S., Bucher G., Schuetz S., Maleszka R., Wimmer E.A., Beeman R.W.,
RA Lorenzen M., Tomoyasu Y., Miller S.C., Grossmann D., Bucher G.;
RT "The genome of the model beetle and pest Tribolium castaneum.";
RL Nature 452:949-955(2008).
RN [2] {ECO:0000313|EMBL:EFA03461.2, ECO:0000313|Proteomes:UP000007266}
RP GENOME REANNOTATION.
RC STRAIN=Georgia GA2 {ECO:0000313|EMBL:EFA03461.2,
RC ECO:0000313|Proteomes:UP000007266};
RX PubMed=19820115; DOI=10.1093/nar/gkp807;
RA Kim H.S., Murphy T., Xia J., Caragea D., Park Y., Beeman R.W.,
RA Lorenzen M.D., Butcher S., Manak J.R., Brown S.J.;
RT "BeetleBase in 2010: revisions to provide comprehensive genomic information
RT for Tribolium castaneum.";
RL Nucleic Acids Res. 38:D437-D442(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, COPII-coated vesicle
CC membrane {ECO:0000256|ARBA:ARBA00004557}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004557}. Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004653}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004653}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the WD repeat SCAP family.
CC {ECO:0000256|ARBA:ARBA00007410}.
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DR EMBL; KQ971343; EFA03461.2; -; Genomic_DNA.
DR RefSeq; XP_008193751.1; XM_008195529.2.
DR RefSeq; XP_015835772.1; XM_015980286.1.
DR AlphaFoldDB; D6WLE2; -.
DR STRING; 7070.D6WLE2; -.
DR EnsemblMetazoa; TC013456_001; TC013456_001; TC013456.
DR GeneID; 663635; -.
DR KEGG; tca:663635; -.
DR eggNOG; KOG1933; Eukaryota.
DR HOGENOM; CLU_006510_0_0_1; -.
DR InParanoid; D6WLE2; -.
DR OMA; IMKQYNV; -.
DR OrthoDB; 2875749at2759; -.
DR Proteomes; UP000007266; Linkage group 5.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032936; C:SREBP-SCAP complex; IBA:GO_Central.
DR GO; GO:0032934; F:sterol binding; IEA:InterPro.
DR GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0045540; P:regulation of cholesterol biosynthetic process; IBA:GO_Central.
DR GO; GO:0032933; P:SREBP signaling pathway; IEA:InterPro.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR030225; SCAP.
DR InterPro; IPR000731; SSD.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR46378; STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN; 1.
DR PANTHER; PTHR46378:SF1; STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN; 1.
DR Pfam; PF12349; Sterol-sensing; 1.
DR Pfam; PF00400; WD40; 4.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF82866; Multidrug efflux transporter AcrB transmembrane domain; 1.
DR SUPFAM; SSF50998; Quinoprotein alcohol dehydrogenase-like; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50156; SSD; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
PE 3: Inferred from homology;
KW Cholesterol metabolism {ECO:0000256|ARBA:ARBA00022548};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Lipid-binding {ECO:0000256|ARBA:ARBA00023121};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000007266};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221};
KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE-
KW ProRule:PRU00221}.
FT TRANSMEM 285..304
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 316..342
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 354..378
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 399..417
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 423..445
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 522..540
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 710..735
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 287..445
FT /note="SSD"
FT /evidence="ECO:0000259|PROSITE:PS50156"
FT REPEAT 772..811
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1139..1178
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REGION 828..848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 828..843
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1260 AA; 143212 MW; FE5B682EE1102137 CRC64;
MNTRGDEPRP RGTFCGKSST LQEKVAQFYY AHGLFCSTYP GALVFVAICA TLLSCYPLLN
LPLPGNSPLQ TWTSSVNSSS NVPPFCYIQQ VVLKSTVLPW GPELHLGDAF RAPLYEAFKL
LEAVRNYQDE STKKTLGHVC LHVESIRKSK SNYKNILPEY SCLVLSPANL WQQDVLKFTQ
DSSLLTTIFN HHNFQKGKTS ITEMMFGMKV FDTGIKRYPL RNRYRIIQYA ITLFFKEYDE
KFVHGLRRKL QSVYSLHQNL ENGTNANFAT LNDTLIIQYP GEINLVEFIP ITLAFLILFL
YFYFSFRKIE LIHSKLGMAF TSVLTVCASL TMTYGLCFFF GLTLNLEGKV VFPYLVLLVG
LENVLCLTKS IVSSPLHLDV KIRIAQGLSK EGWSITKNLL LEITILTFGI FTFVAVIQEF
CIFAVVGLVT DYFMQMIFFS TVLGIDIRRK ENMVERHNLS FRSSLFQSQV FYDKSFGIRR
SKSHPRLSSF PANIVAGQVQ GAQEKKIPKR LKLVNIWTRT RFFHRAFMIL MVIWISNIAY
NSGFIEHFIL NNSYFHEVAE QNSTSLEQNY SSIKLFPLLN TNNSFINKVS YVTHSPLNYN
QNQTFDLENL KHSDYEPWLK LSPQHWSSIV RKYNLSLSGQ YIAILPNIKL SHVIRPEQAV
LLRNPNEKYG EKFQWQALVA ALDPIDFNGM ETGTRSAIPQ SEQPFYPTSV FAMILIAILC
FISVVVLGYT FVVLYRCICS RNYAEWRASW FSEKGEENVE EPVLLEAVPV VLEGHQQEIE
CTATDGVNLV SASLDGQLKV WDSGTGELIA NVDRKAYFQG CFESNCGTSE CDDNSSDYES
GSPPSRGESY LFPRLKHRIN TNFSNVKEQC NSYNAKADFL KTYRELYFVR DLPKAKSRKE
MNRAKENKSI SFSESNQKFC DNGHTKFDSS DAKLSPIWCM DYLENLIVLG CADGRLEFWE
GSSGRLKCIF EDGTDVGISN LKIVGSRIIA SRLYGTLEMF QLQTYNRGHP VDWNFTCAYR
RTHVRTGSVG SVPDHTNLIS QNEDEEDFRC LKVFSVKAHQ QPIICLDCEG GRILTGSQDH
TLKVFRLEDG SPQYTLHGHC GPITCLFIDR ISPATSGSGS QDGMLCVWDL LTGACMYNIQ
AHDGTITSLT YSASYVISLG SDDRICVWER FQGHLLNTIY VSQTFSSQVL MLAPHLVVTA
RSGGLVIWDV RSGECVRTII LGRSPYIFIK QLMLLRDAVV CDYGNQLRIV RFPLITHKFD
//
