ID D7G0C5_ECTSI Unreviewed; 1005 AA.
AC D7G0C5;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 28-JUN-2023, entry version 40.
DE RecName: Full=Fanconi-associated nuclease {ECO:0000256|RuleBase:RU365033};
DE EC=3.1.4.1 {ECO:0000256|RuleBase:RU365033};
GN ORFNames=Esi_0040_0052 {ECO:0000313|EMBL:CBJ26652.1};
OS Ectocarpus siliculosus (Brown alga) (Conferva siliculosa).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; PX clade; Phaeophyceae;
OC Ectocarpales; Ectocarpaceae; Ectocarpus.
OX NCBI_TaxID=2880 {ECO:0000313|EMBL:CBJ26652.1, ECO:0000313|Proteomes:UP000002630};
RN [1] {ECO:0000313|EMBL:CBJ26652.1, ECO:0000313|Proteomes:UP000002630}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ec32 / CCAP1310/4 {ECO:0000313|Proteomes:UP000002630};
RX PubMed=20520714; DOI=10.1038/nature09016;
RA Cock J.M., Sterck L., Rouze P., Scornet D., Allen A.E., Amoutzias G.,
RA Anthouard V., Artiguenave F., Aury J.M., Badger J.H., Beszteri B.,
RA Billiau K., Bonnet E., Bothwell J.H., Bowler C., Boyen C., Brownlee C.,
RA Carrano C.J., Charrier B., Cho G.Y., Coelho S.M., Collen J., Corre E.,
RA Da Silva C., Delage L., Delaroque N., Dittami S.M., Doulbeau S., Elias M.,
RA Farnham G., Gachon C.M., Gschloessl B., Heesch S., Jabbari K., Jubin C.,
RA Kawai H., Kimura K., Kloareg B., Kupper F.C., Lang D., Le Bail A.,
RA Leblanc C., Lerouge P., Lohr M., Lopez P.J., Martens C., Maumus F.,
RA Michel G., Miranda-Saavedra D., Morales J., Moreau H., Motomura T.,
RA Nagasato C., Napoli C.A., Nelson D.R., Nyvall-Collen P., Peters A.F.,
RA Pommier C., Potin P., Poulain J., Quesneville H., Read B., Rensing S.A.,
RA Ritter A., Rousvoal S., Samanta M., Samson G., Schroeder D.C., Segurens B.,
RA Strittmatter M., Tonon T., Tregear J.W., Valentin K., von Dassow P.,
RA Yamagishi T., Van de Peer Y., Wincker P.;
RT "The Ectocarpus genome and the independent evolution of multicellularity in
RT brown algae.";
RL Nature 465:617-621(2010).
CC -!- FUNCTION: Nuclease required for the repair of DNA interstrand cross-
CC links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of
CC DNA and cleaves DNA successively at every third nucleotide, allowing to
CC excise an ICL from one strand through flanking incisions.
CC {ECO:0000256|RuleBase:RU365033}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolytically removes 5'-nucleotides successively from the
CC 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.;
CC EC=3.1.4.1; Evidence={ECO:0000256|ARBA:ARBA00000983,
CC ECO:0000256|RuleBase:RU365033};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365033}.
CC -!- SIMILARITY: Belongs to the FAN1 family. {ECO:0000256|ARBA:ARBA00005533,
CC ECO:0000256|RuleBase:RU365033}.
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DR EMBL; FN648597; CBJ26652.1; -; Genomic_DNA.
DR AlphaFoldDB; D7G0C5; -.
DR STRING; 2880.D7G0C5; -.
DR EnsemblProtists; CBJ26652; CBJ26652; Esi_0040_0052.
DR eggNOG; KOG2143; Eukaryota.
DR InParanoid; D7G0C5; -.
DR OrthoDB; 38749at2759; -.
DR Proteomes; UP000002630; Chromosome LG15.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004528; F:phosphodiesterase I activity; IEA:UniProtKB-EC.
DR GO; GO:0036297; P:interstrand cross-link repair; IEA:InterPro.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR033315; Fan1-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR014883; VRR_NUC.
DR PANTHER; PTHR15749; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR PANTHER; PTHR15749:SF4; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR Pfam; PF08774; VRR_NUC; 1.
DR SMART; SM00990; VRR_NUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU365033};
KW DNA repair {ECO:0000256|RuleBase:RU365033};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU365033};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365033};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|RuleBase:RU365033};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365033};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU365033};
KW Nucleus {ECO:0000256|RuleBase:RU365033};
KW Reference proteome {ECO:0000313|Proteomes:UP000002630}.
FT DOMAIN 840..992
FT /note="VRR-NUC"
FT /evidence="ECO:0000259|SMART:SM00990"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 78..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 223..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 714..740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1005 AA; 110350 MW; BF2D5247AEB8452E CRC64;
MELGGSIATP LPPPTVADGW GGKPALQHYP HPSDAAATAG VGVQRCEWGQ TTQQVRRERQ
QQPQEFPPAV LALQLQEWGE TQQQQPRQEA SPTLRRQQWG RGASAATHSL GGGCLRDDQE
AGHDQPPFVM MGMLDDSRAL LLPTTRERRE VMGSLSSASV PVTWTDSTGQ SRTAILTRTK
AKPKPCNPLP MLANLLAWMR ELPPGALPEN RLRIRLETLL ALQSPSKSVS RQSSTIGKNT
NSNSGHTNRD RGDTTTSTAT ATNNNNSNTT SNHDGRASGG GDGDGDLSLG AAWLLAHLMF
GMDGGGKATG PFEREILNGK VSLSGAEANA MPLLLCPLIG WSPRPREVIQ AMSEGAGILC
AYSVELAAFE TQTGICGKAA DELVHAGFAR KVEFGEMLAL DQKMEDACEL VLKIFLCNWD
VDREVRAQVA LGLTKTGPVA SGLCPPDIPS LFSGRQKWRS STTAVRGGDE RSSCRGRMGG
LVGDLCVRLH SWCHQVMRAG RDLRVLLLLQ VLKGETAGDL TSQREWTGPM AQAFGLLGGA
EVSYHKRHRD SIGGTASAGW VASLSKEERL LEVACLRLKQ AMKWVLVVVR GLAEESQKKG
EHDEAVLIFR LLVRRDQGLA GPMYRDTAPD LVWAADWVTC DQRGFAYTRL AINLKTHLKR
PREEQLDVVK AACDDDCVRG GDRVDLWERY QGLHKKCGDR QTASLRGRQR SPLWDPKVED
IHPPVETQKQ GSQGTLGEKG GRKLYYYNDA APGERRWSEA RSHNVEEVVI RERYSAGGWL
GLHGESKLIM FILMLLLWEE VFDSGVEAAV PHCIMISPLD TSDKFFTTRR EGLPDRLATI
GQYADRQLFS DVGQRYDRLR GVRSGWCDWN VFPKQQIMEI CVAFGGKRLS DLLKTLTNNL
ADMDKGFPDV VLRRPKGADF GAGGSSSGGG RDERDWKART HFGLGWQGHG HGWEVEVVEV
KSKGDTISKE QKAWLAELED IGVPAMVSRV VAENEAVPKK RSRSR
//
