ID D7KBC1_ARALL Unreviewed; 501 AA.
AC D7KBC1;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 24-JAN-2024, entry version 57.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=ARALYDRAFT_887421 {ECO:0000313|EMBL:EFH68384.1};
OS Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=81972 {ECO:0000313|Proteomes:UP000008694};
RN [1] {ECO:0000313|Proteomes:UP000008694}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. MN47 {ECO:0000313|Proteomes:UP000008694};
RX PubMed=21478890; DOI=10.1038/ng.807;
RA Hu T.T., Pattyn P., Bakker E.G., Cao J., Cheng J.-F., Clark R.M.,
RA Fahlgren N., Fawcett J.A., Grimwood J., Gundlach H., Haberer G.,
RA Hollister J.D., Ossowski S., Ottilar R.P., Salamov A.A., Schneeberger K.,
RA Spannagl M., Wang X., Yang L., Nasrallah M.E., Bergelson J.,
RA Carrington J.C., Gaut B.S., Schmutz J., Mayer K.F.X., Van de Peer Y.,
RA Grigoriev I.V., Nordborg M., Weigel D., Guo Y.-L.;
RT "The Arabidopsis lyrata genome sequence and the basis of rapid genome size
RT change.";
RL Nat. Genet. 43:476-481(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; GL348713; EFH68384.1; -; Genomic_DNA.
DR RefSeq; XP_002892125.1; XM_002892079.1.
DR AlphaFoldDB; D7KBC1; -.
DR STRING; 81972.D7KBC1; -.
DR EnsemblPlants; scaffold_100215.1; scaffold_100215.1; scaffold_100215.1.
DR GeneID; 9328187; -.
DR Gramene; scaffold_100215.1; scaffold_100215.1; scaffold_100215.1.
DR KEGG; aly:9328187; -.
DR eggNOG; ENOG502QPI6; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000008694; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009624; P:response to nematode; IEA:EnsemblPlants.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF124; ENDOGLUCANASE 1; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000008694};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 26..501
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5005126817"
FT DOMAIN 44..495
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 422
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 473
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 482
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 501 AA; 55411 MW; 15CCB4D66AF2A9EE CRC64;
MALYLYSSRL IICLSFLLLI TNGFSSSSSR SSIHHRHHLD NHNYKDALSK SILFFEGQRS
GKLPPNQRMT WRSNSGLSDG SALNVDLVGG YYDAGDNMKF GFPMAFTTTM LSWSLIEFGG
LMKSELPNAK DAVRWATDFL LKATSHPDTI YVQVGDPNMD HACWERPEDM DTPRSVFKVD
KNNPGSDIAG EIAAALAAAS IVFGRCDPSY SNLLLQRAIT VFTFADKYRG PYSAGLAPEV
CPFYCSYSGY QDELLWGAAW LHKATNNPTY LNYIKANGQI LGADEFDNMF SWDNKHVGAR
ILLSKEFLIQ KVKSLEEYKE HADSFICSVL PGASSSQYTP GGLLFKMGES NMQYVTSTSF
LLLTYAKYLT SARTVAYCGG SVVTPARLRS IARKQVDYLL GDNPLKMSYM VGYGLKYPRR
IHHRGSSLPS VAVHPTRIQC HDGFSMFTSQ SPNPNDLVGA VVGGPDQNDQ FPDERSDYGR
SEPATYINAP LVGALACLAR S
//
