ID D7KBI2_ARALL Unreviewed; 504 AA.
AC D7KBI2;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=apyrase {ECO:0000256|ARBA:ARBA00012148};
DE EC=3.6.1.5 {ECO:0000256|ARBA:ARBA00012148};
DE AltName: Full=ATP-diphosphatase {ECO:0000256|ARBA:ARBA00031428};
DE AltName: Full=ATP-diphosphohydrolase {ECO:0000256|ARBA:ARBA00032306};
DE AltName: Full=Adenosine diphosphatase {ECO:0000256|ARBA:ARBA00030084};
DE AltName: Full=NTPDase {ECO:0000256|ARBA:ARBA00031370};
GN ORFNames=ARALYDRAFT_679147 {ECO:0000313|EMBL:EFH66303.1};
OS Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=81972 {ECO:0000313|Proteomes:UP000008694};
RN [1] {ECO:0000313|Proteomes:UP000008694}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. MN47 {ECO:0000313|Proteomes:UP000008694};
RX PubMed=21478890; DOI=10.1038/ng.807;
RA Hu T.T., Pattyn P., Bakker E.G., Cao J., Cheng J.-F., Clark R.M.,
RA Fahlgren N., Fawcett J.A., Grimwood J., Gundlach H., Haberer G.,
RA Hollister J.D., Ossowski S., Ottilar R.P., Salamov A.A., Schneeberger K.,
RA Spannagl M., Wang X., Yang L., Nasrallah M.E., Bergelson J.,
RA Carrington J.C., Gaut B.S., Schmutz J., Mayer K.F.X., Van de Peer Y.,
RA Grigoriev I.V., Nordborg M., Weigel D., Guo Y.-L.;
RT "The Arabidopsis lyrata genome sequence and the basis of rapid genome size
RT change.";
RL Nat. Genet. 43:476-481(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + 2 H2O = a ribonucleoside
CC 5'-phosphate + 2 H(+) + 2 phosphate; Xref=Rhea:RHEA:36795,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58043, ChEBI:CHEBI:61557; EC=3.6.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000211};
CC -!- SIMILARITY: Belongs to the GDA1/CD39 NTPase family.
CC {ECO:0000256|ARBA:ARBA00009283}.
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DR EMBL; GL348713; EFH66303.1; -; Genomic_DNA.
DR RefSeq; XP_002890044.1; XM_002889998.1.
DR AlphaFoldDB; D7KBI2; -.
DR STRING; 81972.D7KBI2; -.
DR EnsemblPlants; Al_scaffold_0001_1470; Al_scaffold_0001_1470; Al_scaffold_0001_1470.
DR Gramene; Al_scaffold_0001_1470; Al_scaffold_0001_1470; Al_scaffold_0001_1470.
DR eggNOG; KOG1386; Eukaryota.
DR HOGENOM; CLU_010246_5_1_1; -.
DR Proteomes; UP000008694; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004050; F:apyrase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.30.420.150; Exopolyphosphatase. Domain 2; 1.
DR InterPro; IPR000407; GDA1_CD39_NTPase.
DR PANTHER; PTHR11782; ADENOSINE/GUANOSINE DIPHOSPHATASE; 1.
DR PANTHER; PTHR11782:SF100; APYRASE 3-RELATED; 1.
DR Pfam; PF01150; GDA1_CD39; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000008694};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 47..69
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 206
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-1"
FT BINDING 236..240
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-2"
SQ SEQUENCE 504 AA; 55595 MW; 642F1E219B1D507A CRC64;
MQRFNARSGL KFKSDMRDPP EVQTSPGNHR SSPSTVAKPK WKHTKSIVFV IVSCVTIALG
LFFVCYSISR SGRNRRVSLR YSIIIDGGSS GTRVHVFGYR IQSGEPVFDF GEESYGSLKL
SPGLSAYAEN PEGVSESVTE LVEFAKRRVP KGKLKKSDIR LMATAGMRLL ELPVQEQILD
VTRRVLRSSG FEFREEWASV ISGSDEGVYA WVVANHALGS LGGEPLKTTG IVELGGASTQ
VTFVSSELVP SEFSRTLDYG NVSYNLYSHS FLDFGQDAAQ EKLSEFLHNS AAANSTGEGI
VPDPCTPKGY ILKTNLQKDL PGFLADKGKF IATLQAAGNF SECRSAAFAM LQEGKWKCTY
KHCSIGSIFT PNLQGSFLAT ENFFHTSKFF GLGEKDWLSE MILAGNRFCG EDWSKLKVKY
PTFKDENLLR YCFSSAYIIS MLHDSLGVAL DDKRIKYASK AGDDDIPLDW ALGAFILNTA
TATFDYSGKS RKILDLSNVA KYKT
//
