ID D7KNA6_ARALL Unreviewed; 663 AA.
AC D7KNA6;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 24-JAN-2024, entry version 64.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
GN ORFNames=ARALYDRAFT_888545 {ECO:0000313|EMBL:EFH66182.1};
OS Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=81972 {ECO:0000313|Proteomes:UP000008694};
RN [1] {ECO:0000313|Proteomes:UP000008694}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. MN47 {ECO:0000313|Proteomes:UP000008694};
RX PubMed=21478890; DOI=10.1038/ng.807;
RA Hu T.T., Pattyn P., Bakker E.G., Cao J., Cheng J.-F., Clark R.M.,
RA Fahlgren N., Fawcett J.A., Grimwood J., Gundlach H., Haberer G.,
RA Hollister J.D., Ossowski S., Ottilar R.P., Salamov A.A., Schneeberger K.,
RA Spannagl M., Wang X., Yang L., Nasrallah M.E., Bergelson J.,
RA Carrington J.C., Gaut B.S., Schmutz J., Mayer K.F.X., Van de Peer Y.,
RA Grigoriev I.V., Nordborg M., Weigel D., Guo Y.-L.;
RT "The Arabidopsis lyrata genome sequence and the basis of rapid genome size
RT change.";
RL Nat. Genet. 43:476-481(2011).
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|ARBA:ARBA00004914}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. Vacuole
CC lumen {ECO:0000256|ARBA:ARBA00004410}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; GL348713; EFH66182.1; -; Genomic_DNA.
DR RefSeq; XP_002889923.1; XM_002889877.1.
DR AlphaFoldDB; D7KNA6; -.
DR STRING; 81972.D7KNA6; -.
DR EnsemblPlants; scaffold_101339.1; scaffold_101339.1; scaffold_101339.1.
DR GeneID; 9325986; -.
DR Gramene; scaffold_101339.1; scaffold_101339.1; scaffold_101339.1.
DR KEGG; aly:9325986; -.
DR eggNOG; KOG0228; Eukaryota.
DR HOGENOM; CLU_001528_6_1_1; -.
DR OrthoDB; 315037at2759; -.
DR Proteomes; UP000008694; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; IEA:EnsemblPlants.
DR GO; GO:0005775; C:vacuolar lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071370; P:cellular response to gibberellin stimulus; IEA:EnsemblPlants.
DR GO; GO:0080022; P:primary root development; IEA:EnsemblPlants.
DR GO; GO:0009617; P:response to bacterium; IEA:EnsemblPlants.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF1; ACID BETA-FRUCTOFURANOSIDASE 4, VACUOLAR; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000008694};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 43..62
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 21..116
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 124..444
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 447..643
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 663 AA; 73835 MW; 19D97715F00B1E6C CRC64;
MASSDALLPI SAREEEPLCP YTRLPMADPN QETHGRRRRP FKGLLAVSFG LLFIAFYVAL
IATHDGSRSN DAGIDETETI TSRARLAGVS EKSNDGLWKL SGDRNTPAFE WNNSMLSWQR
TAFHFQPEQN WMNDPNGPLF YKGWYHFFYQ YNPNAAVWGD IVWGHAVSRD LIHWVHLPIA
MVADQWYDSN GVWTGSATIL PDGSIVMLYT GSTDKAVQVQ NLAYPEDPSD PLLLKWVKFP
GNPVLVPPPG ILPKDFRDPT TAWKTSEGKW RITIGSKLNR TGISLVYDTT DFKTYEKLDT
LLHRVPNTGM WECVDFYPVS KTAVNGLDTS VKGPDVKHIV KASMDDTRFD HYAVGTYFDS
NGTWIPDDPT IDVGMSTSLR YDYGKFYASK TFYDQNKGRR VLWSWIGESD SEAADVQKGW
SSVQGIPRTV VLDTKTGKNL VQWPVEEIKS LRLSSKQFDI KVGPGSVVPV DVGSAAQLDI
EAEFEINKES LDKILGNASV MAEAEEFSCE KSGGSTVRGA LGPFGFSVLA TESLSEQTPV
YFYVAKGKDS ELKTFFCIDT SRSSVANDVV KLIYGSVVPV LKGEKLTMRI LVDHSIVEAF
GQGGRTCITS RVYPTSAIYG AAKLFLFNNA LDATITASFR VWQMNSAFIH PYTEEAFRAL
TRT
//
