GenomeNet

Database: UniProt
Entry: D7TAQ4_VITVI
LinkDB: D7TAQ4_VITVI
Original site: D7TAQ4_VITVI 
ID   D7TAQ4_VITVI            Unreviewed;       835 AA.
AC   D7TAQ4;
DT   10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT   10-AUG-2010, sequence version 1.
DT   31-JUL-2019, entry version 68.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   OrderedLocusNames=VIT_01s0010g02760 {ECO:0000313|EMBL:CBI27577.3};
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; Vitales; Vitaceae; Viteae; Vitis.
OX   NCBI_TaxID=29760 {ECO:0000313|Proteomes:UP000009183};
RN   [1] {ECO:0000313|Proteomes:UP000009183}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Pinot noir / PN40024 {ECO:0000313|Proteomes:UP000009183};
RX   PubMed=17721507; DOI=10.1038/nature06148;
RG   The French-Italian Public Consortium for Grapevine Genome Characterization.;
RA   Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C.,
RA   Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A.,
RA   Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D.,
RA   Poulain J., Bruyere C., Billault A., Segurens B., Gouyvenoux M.,
RA   Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C.,
RA   Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I.,
RA   Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G.,
RA   Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D.,
RA   Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F.,
RA   Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F.,
RA   Weissenbach J., Quetier F., Wincker P.;
RT   "The grapevine genome sequence suggests ancestral hexaploidization in
RT   major angiosperm phyla.";
RL   Nature 449:463-467(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; FN595754; CBI27577.3; -; Genomic_DNA.
DR   RefSeq; XP_002266856.1; XM_002266820.3.
DR   RefSeq; XP_010655403.1; XM_010657101.2.
DR   STRING; 29760.VIT_01s0010g02760.t01; -.
DR   EnsemblPlants; VIT_01s0010g02760.t01; VIT_01s0010g02760.t01; VIT_01s0010g02760.
DR   GeneID; 100263023; -.
DR   Gramene; VIT_01s0010g02760.t01; VIT_01s0010g02760.t01; VIT_01s0010g02760.
DR   KEGG; vvi:100263023; -.
DR   eggNOG; ENOG410IGM6; Eukaryota.
DR   eggNOG; ENOG410JX8F; Eukaryota.
DR   eggNOG; COG0804; LUCA.
DR   eggNOG; COG0831; LUCA.
DR   eggNOG; COG0832; LUCA.
DR   HOGENOM; HOG000075064; -.
DR   InParanoid; D7TAQ4; -.
DR   KO; K01427; -.
DR   OMA; GFDSHIH; -.
DR   OrthoDB; 183108at2759; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000009183; Chromosome 1.
DR   ExpressionAtlas; D7TAQ4; baseline.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR040881; Urease_linker.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   Pfam; PF18473; Urease_linker; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000009183};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000009183}.
FT   DOMAIN      397    835       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    588    588       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       402    402       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       404    404       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       485    485       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       485    485       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       514    514       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       540    540       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       628    628       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     487    487       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     485    485       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   835 AA;  89661 MW;  F0545AC6690B6E11 CRC64;
     MKLSPREVDK LLLHNAGFLA QKRLASGLRL NYTEAVALIA SQILAFVREG EKTVAELMDI
     GKQLLGRRQV LPAVPHLLHT VQVEGTFPDG TKLVTVHDAI ASENGNLDLA LHGSFLPVPS
     VDKFPDMEDD RIPGEIRYGG GTIMLNSCRK AIVLRVTNTG DRPIQVGSHY HFIEVNPALV
     FDRRKAHGMR LNIPAGTATR FEPGETKRVS LVRIGGKQVI RGGNCIIDGP VDDTNITAVM
     ESESMVRFGH SEEAHVSEGV IGEDPDLAIR MSHEAYANMY GPTTGDKIRL GDTELYAEIE
     SDFAVYGDEC VFGGGKVIRD GMGQACMYAA AECVDTVITN AVVIDYTGIF KADIGIKDGL
     IVSLGKAGNP DIMHGAHMII GVSTEVIAGE GMIVTAGAID CHVHFICPQL AYEAISSGIT
     TLVGGGTGPA DGTRATTCTP AASHMKFMLQ STDDLPLNFG FTGKGNSAKP DGLHEIIRAG
     AMGLKLHEDW GTTPAAIDNC LTVAEQYDIQ VNIHTDTLNE SGFVEHTIAA FKDRTIHTYH
     SEGAGGGHAP DIIKVCGVKN VLPSSTNPTR PFTSNTIDEH LDMLMVCHHL DKDIPEDVAF
     AESRIRAETI AAEDILHDMG AISIIASDSQ AMGRIGEVII RTWQTAHKMK LQRGSLDASG
     VDNDNLRIKR YIAKYTINPA IANGFSRFVG SIEVGKVADL VLWNPSFFGA KPEMVIKGGV
     IAWANMGDPN ASIPTPEPVM MRPMFGAFGK AGSANSIAFV SKVAAECGIK THYGLTKRVE
     AVGNVRRLTK LDMKLNDALP VITVDPETYT VTADGVTLSC PAATTVPLSR NYFLF
//
DBGET integrated database retrieval system