ID D8EWL4_9DELT Unreviewed; 562 AA.
AC D8EWL4;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:EFK12075.1};
GN ORFNames=NPH_2906 {ECO:0000313|EMBL:EFK12075.1};
OS delta proteobacterium NaphS2.
OC Bacteria; Deltaproteobacteria.
OX NCBI_TaxID=88274 {ECO:0000313|EMBL:EFK12075.1, ECO:0000313|Proteomes:UP000003834};
RN [1] {ECO:0000313|EMBL:EFK12075.1, ECO:0000313|Proteomes:UP000003834}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NaphS2 {ECO:0000313|EMBL:EFK12075.1,
RC ECO:0000313|Proteomes:UP000003834};
RX PubMed=21124915; DOI=10.1371/journal.pone.0014072;
RA Didonato R.J., Young N.D., Butler J.E., Chin K.J., Hixson K.K., Mouser P.,
RA Lipton M.S., Deboy R., Methe B.A.;
RT "Genome Sequence of the Deltaproteobacterial Strain NaphS2 and Analysis of
RT Differential Gene Expression during Anaerobic Growth on Naphthalene.";
RL PLoS ONE 5:E14072-E14072(2010).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFK12075.1}.
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DR EMBL; ADZZ01000049; EFK12075.1; -; Genomic_DNA.
DR AlphaFoldDB; D8EWL4; -.
DR OrthoDB; 9804952at2; -.
DR Proteomes; UP000003834; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000003834}.
FT DOMAIN 1..143
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 177..394
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 562 AA; 64673 MW; C665A633FFCDA4F1 CRC64;
MKILLVYPPF LEARIHEEEV NVPPLGLYYV GALLREQGYD VEILNWAHLD HISEAVTRVI
EEKDPDIIGL SVLQANRWGA LEIAKTAKSI DSEITVALGG IAATFLWKHF LTHFEAIDFC
VLGEGEYPFL ELVKSIETGN RKRIRSIKGI AFRSNGRPIK TGRSLPIKNL DNLPIPSKYF
DYQHVAFTRG CPAQCTFCGS PKFWGRRVRF HSATYFVKQL TYLYERGIRF FYFSDDTFLI
NKERVIDICR EIIERDIKIS WFAISRVDLV DEEVLRWMRL AGCTQISYGV ESGSEEIRDF
LNKNIRSDDI KKAFQLTTRY GILARAYFIY GSPGETMHTV KETIDLIRDI KPLGAIFYIL
DIFPGTILYD MFLKQSGQTD DIWLQKIEDI LYFETDPRLT REMIFAFGRE LRSAFHEALP
GFAETIELTD GKAFRESNAD FLSRLAMTFS HGDYSKIDAI PRKDETAVRL YKRSLEYAPN
DRAYLGLGML KQRDRAFNES VAVLDKGLAF FPDNDQLKLC KGLSLMNLRA FKKALFCFES
MENPAPAKPY VNECRKMLSS RA
//
