ID D8L9G8_WHEAT Unreviewed; 269 AA.
AC D8L9G8;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Steroid 5-alpha-reductase DET2 {ECO:0000256|PIRNR:PIRNR015596};
DE EC=1.3.1.22 {ECO:0000256|PIRNR:PIRNR015596};
GN ORFNames=CFC21_042926 {ECO:0000313|EMBL:KAF7031622.1},
GN TAA_ctg0079b.00060.1 {ECO:0000313|EMBL:CBH32517.1},
GN TRAES_3BF039900020CFD_c1 {ECO:0000313|EMBL:CDM85027.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EMBL:CBH32517.1};
RN [1] {ECO:0000313|EMBL:CBH32517.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=20581307; DOI=10.1105/tpc.110.074187;
RG Genoscope -,C.E.A.;
RA Choulet F., Wicker T., Rustenholz C., Paux E., Salse J., Leroy P.,
RA Schlub S., Le Paslier M.C., Magdelenat G., Gonthier C., Couloux A.,
RA Budak H., Breen J., Kong X., Gut M., Brunel D., Anderson J.A., Gill B.S.,
RA Appels R., Keller B., Feuillet C.;
RT "Megabase level sequencing reveals contrasted organization and evolution
RT patterns of the wheat gene and transposable element spaces.";
RL Plant Cell 22:1686-1701(2010).
RN [2] {ECO:0000313|EMBL:CDM85027.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25035497; DOI=10.1126/science.1249721;
RA Choulet F., Alberti A., Theil S., Glover N., Barbe V., Daron J.,
RA Pingault L., Sourdille P., Couloux A., Paux E., Leroy P., Mangenot S.,
RA Guilhot N., Le Gouis J., Balfourier F., Alaux M., Jamilloux V., Poulain J.,
RA Durand C., Bellec A., Gaspin C., Safar J., Dolezel J., Rogers J.,
RA Vandepoele K., Aury J.M., Mayer K., Berges H., Quesneville H., Wincker P.,
RA Feuillet C.;
RT "Structural and functional partitioning of bread wheat chromosome 3B.";
RL Science 345:1249721-1249721(2014).
RN [3] {ECO:0000313|EMBL:KAF7031622.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7031622.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [4] {ECO:0000313|EnsemblPlants:TraesCS3B02G397800.1.cds1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS3B02G397800.1.cds1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [5] {ECO:0000313|EnsemblPlants:TraesCS3B02G397800.1.cds1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [6] {ECO:0000313|EMBL:KAF7031622.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7031622.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in a reduction step in the biosynthesis of the plant
CC steroid, brassinolide. {ECO:0000256|PIRNR:PIRNR015596}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid +
CC H(+) + NADPH; Xref=Rhea:RHEA:54384, ChEBI:CHEBI:13601,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47909, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000256|PIRNR:PIRNR015596};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000256|PIRNR:PIRNR015596}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
CC {ECO:0000256|ARBA:ARBA00007742, ECO:0000256|PIRNR:PIRNR015596}.
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DR EMBL; FN564427; CBH32517.1; -; Genomic_DNA.
DR EMBL; HG670306; CDM85027.1; -; Genomic_DNA.
DR EMBL; CM022218; KAF7031622.1; -; Genomic_DNA.
DR SMR; D8L9G8; -.
DR STRING; 4565.D8L9G8; -.
DR EnsemblPlants; TraesCS3B02G397800.1; TraesCS3B02G397800.1.cds1; TraesCS3B02G397800.
DR Gramene; TraesCAD_scaffold_033568_01G000100.1; TraesCAD_scaffold_033568_01G000100.1; TraesCAD_scaffold_033568_01G000100.
DR Gramene; TraesCLE_scaffold_031539_01G000200.1; TraesCLE_scaffold_031539_01G000200.1; TraesCLE_scaffold_031539_01G000200.
DR Gramene; TraesCS3B02G397800.1; TraesCS3B02G397800.1.cds1; TraesCS3B02G397800.
DR Gramene; TraesCS3B03G0993100.1; TraesCS3B03G0993100.1.CDS1; TraesCS3B03G0993100.
DR Gramene; TraesKAR3B01G0417080.1; cds.TraesKAR3B01G0417080.1; TraesKAR3B01G0417080.
DR Gramene; TraesPAR_scaffold_004330_01G000100.1; TraesPAR_scaffold_004330_01G000100.1; TraesPAR_scaffold_004330_01G000100.
DR Gramene; TraesROB_scaffold_000014_01G000800.1; TraesROB_scaffold_000014_01G000800.1; TraesROB_scaffold_000014_01G000800.
DR HOGENOM; CLU_065395_1_0_1; -.
DR OMA; HRDYQQR; -.
DR OrthoDB; 2896758at2759; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000019116; Chromosome 3B.
DR Proteomes; UP000815260; Chromosome 3B.
DR ExpressionAtlas; D8L9G8; baseline.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0047751; F:3-oxo-5alpha-steroid 4-dehydrogenase (NADP+); IEA:UniProtKB-EC.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR Gene3D; 1.20.120.1630; -; 1.
DR InterPro; IPR016636; 3-oxo-5-alpha-steroid_4-DH.
DR InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR InterPro; IPR039357; SRD5A/TECR.
DR PANTHER; PTHR10556; 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE; 1.
DR PANTHER; PTHR10556:SF43; 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 2; 1.
DR Pfam; PF02544; Steroid_dh; 1.
DR PIRSF; PIRSF015596; 5_alpha-SR2; 1.
DR PROSITE; PS50244; S5A_REDUCTASE; 1.
PE 3: Inferred from homology;
KW Brassinosteroid biosynthesis {ECO:0000256|PIRNR:PIRNR015596};
KW Lipid biosynthesis {ECO:0000256|PIRNR:PIRNR015596};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR015596};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR015596};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Steroid biosynthesis {ECO:0000256|PIRNR:PIRNR015596};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|PIRNR:PIRNR015596};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|PIRNR:PIRNR015596}.
FT TRANSMEM 14..36
FT /note="Helical"
FT /evidence="ECO:0000256|PIRNR:PIRNR015596"
FT TRANSMEM 77..99
FT /note="Helical"
FT /evidence="ECO:0000256|PIRNR:PIRNR015596"
FT TRANSMEM 119..140
FT /note="Helical"
FT /evidence="ECO:0000256|PIRNR:PIRNR015596"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000256|PIRNR:PIRNR015596"
FT DOMAIN 164..243
FT /note="Steroid 5-alpha reductase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50244"
SQ SEQUENCE 269 AA; 29125 MW; 70667000339ADA8E CRC64;
MSDQAAGAGG GDALFSRCLL ALYVISPVTV FLLRFVSAPY GKLSRPGWGP AIPAALAWCA
MESPTLWLPP LVSPLPLLLA AAAASGSPLA ALPTALYALH YVNRTVLHPL RILRLRRAAA
PVPVLVAACG FGFNLLNAYV QARSWALHAG RPASAFALAR CLAGLALFAW GMRVNVAADK
ELLRLKEAGG GYKIPRGGWF DAVTCPNYFG EIVEWLGYCL VAWSPASWAF FLYTCANLMP
RARDHRQWYL NKFGGEYPAS RKAVIPYIY
//
