ID   D8M6J2_BLAHO            Unreviewed;       399 AA.
AC   D8M6J2;
DT   05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT   05-OCT-2010, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   SubName: Full=Pyruvate Dehydrogenase E1 (Subunit ?) {ECO:0000313|EMBL:CBK23410.2};
GN   ORFNames=GSBLH_T00003293001 {ECO:0000313|EMBL:CBK23410.2};
OS   Blastocystis hominis.
OC   Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Opalinata; Blastocystidae;
OC   Blastocystis.
OX   NCBI_TaxID=12968 {ECO:0000313|EMBL:CBK23410.2};
RN   [1] {ECO:0000313|EMBL:CBK23410.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Singapore isolate B {ECO:0000313|EMBL:CBK23410.2};
RA   Wincker P.;
RT   "Sequencing and annotation of the Blastocystis hominis genome.";
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC         Evidence={ECO:0000256|ARBA:ARBA00001964};
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DR   EMBL; FN668661; CBK23410.2; -; Genomic_DNA.
DR   RefSeq; XP_012897458.1; XM_013042004.1.
DR   AlphaFoldDB; D8M6J2; -.
DR   EnsemblProtists; CBK23410; CBK23410; GSBLH_T00003293001.
DR   GeneID; 24920398; -.
DR   InParanoid; D8M6J2; -.
DR   OMA; HGIVGGQ; -.
DR   OrthoDB; 166915at2759; -.
DR   Proteomes; UP000008312; Unassembled WGS sequence.
DR   GO; GO:0016624; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor; IEA:InterPro.
DR   CDD; cd02000; TPP_E1_PDC_ADC_BCADC; 1.
DR   Gene3D; 3.40.50.970; -; 1.
DR   InterPro; IPR001017; DH_E1.
DR   InterPro; IPR029061; THDP-binding.
DR   PANTHER; PTHR11516:SF60; PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR11516; PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR; 1.
DR   Pfam; PF00676; E1_dh; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 1.
PE   4: Predicted;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Pyruvate {ECO:0000313|EMBL:CBK23410.2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008312}.
FT   DOMAIN          55..356
FT                   /note="Dehydrogenase E1 component"
FT                   /evidence="ECO:0000259|Pfam:PF00676"
SQ   SEQUENCE   399 AA;  44828 MW;  1AB48EC8504A20DF CRC64;
     MLTCAVGKTL PRQLIRAFSS KTFTVKLPEF KVHHTDPLPT TTEVTEEECL RIARNMVTMR
     RMEIESDPLY VMRKIRGFLH LYDGEEACGT GINEAIKPHD DWITSYRCHG VEFLRMGAGE
     AGVKAVINEL LGHASGSAHG KGGSMHMYEP DKNFFGGSGI VGAQTPVGTG LAFAEQYLHM
     LNNRDKSVPE GETNICVTMF GDGASNQGQV WESANMAKLW HLPQIFVIEN NQYGMGTSTE
     RSSSSTEYYM MGKHHIAGIQ ADGNNVFAVR EACRRAREIC VAGNGPIFLE LKTYRYHGHS
     MSDPGVTYRT RDEIQNVRQT RDSVNYIGHI LMENGIMDEK QWKEFQNSIK KEVKGWVNDC
     LKETPPEDSA LFTDVFYGET PDFIRNIEYC ESKSKTPLL
//