ID D8P7K2_9BACT Unreviewed; 569 AA.
AC D8P7K2;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=NIDE3670 {ECO:0000313|EMBL:CBK43348.1};
OS Nitrospira defluvii.
OC Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
OC Nitrospira.
OX NCBI_TaxID=330214 {ECO:0000313|EMBL:CBK43348.1, ECO:0000313|Proteomes:UP000001660};
RN [1] {ECO:0000313|EMBL:CBK43348.1, ECO:0000313|Proteomes:UP000001660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20624973; DOI=10.1073/pnas.1003860107;
RA Lucker S., Wagner M., Maixner F., Pelletier E., Koch H., Vacherie B.,
RA Rattei T., Sinninghe Damste J., Spieck E., Le Paslier D., Daims H.;
RT "A Nitrospira metagenome illuminates the physiology and evolution of
RT globally important nitrite-oxidizing bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 0:13479-13484(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; FP929003; CBK43348.1; -; Genomic_DNA.
DR AlphaFoldDB; D8P7K2; -.
DR STRING; 330214.NIDE3670; -.
DR REBASE; 27071; M.NdeGORF3670P.
DR KEGG; nde:NIDE3670; -.
DR eggNOG; COG0827; Bacteria.
DR HOGENOM; CLU_034179_0_0_0; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000001660; Chromosome.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR011639; MethylTrfase_TaqI-like_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF07669; Eco57I; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:CBK43348.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001660};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CBK43348.1}.
FT DOMAIN 154..263
FT /note="Type II methyltransferase M.TaqI-like"
FT /evidence="ECO:0000259|Pfam:PF07669"
FT DOMAIN 421..520
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
SQ SEQUENCE 569 AA; 64248 MW; 25D5B0778C3EBF0B CRC64;
MADNQTISAT VTRGIQLQLI PPLKRPCSVP AIEPKGVVYT KRWVVELLLD LSGYCSDKNL
VDTLAIEPAT GDGAFLGPMI ERLIESCGNL GRSLAECEHS LLAYELDEKS AARARAVAQN
ILTNRGVKRP LAKRLAEAWV LNRDYLLDAD GTQADFIIGN PPYIRLEDIP EESASIYRDA
YPTMRGRADL YVAFFEAALR QLKRSGLCAF ICADRWMRNQ YGAELRRLIS SAYNVEMLLS
MHHANAFDDE VDAYPAITII RHNRQQSTIV ARADQEAENI QPKLLATMLQ TNNPNILPHG
IHRAVVNTWF KGAAPWPCHS PEQLALLRKL EDQFPPLELN AKVGIGVATG SDRVFITTDA
ELVEPSRLLK LALAKDLSDG TVRWSGHYLV NPWNHDGLVD LKAYPKLQAY YERHAAALKK
RHTAEKSTSK WYKTIDRVNH ALTSAHKLYI PDIKNTLEPV LDQGNTYPHH NLYFIQSDEW
DLEVLGGLLM SKVGQFFIES YGIRMRGGYL RFQAQYLRRI RVPLPTTLSA RQSHELRDAF
RHRDKHRATQ VTLAVYGINF PTLEAALEH
//
