ID D8Q936_SCHCM Unreviewed; 546 AA.
AC D8Q936;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=guanosine-diphosphatase {ECO:0000256|ARBA:ARBA00038903};
DE EC=3.6.1.42 {ECO:0000256|ARBA:ARBA00038903};
GN ORFNames=SCHCODRAFT_57532 {ECO:0000313|EMBL:EFI95582.1};
OS Schizophyllum commune (strain H4-8 / FGSC 9210) (Split gill fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Schizophyllaceae; Schizophyllum.
OX NCBI_TaxID=578458 {ECO:0000313|Proteomes:UP000007431};
RN [1] {ECO:0000313|EMBL:EFI95582.1, ECO:0000313|Proteomes:UP000007431}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H4-8 / FGSC 9210 {ECO:0000313|Proteomes:UP000007431};
RX PubMed=20622885; DOI=10.1038/nbt.1643;
RA Ohm R.A., de Jong J.F., Lugones L.G., Aerts A., Kothe E., Stajich J.E.,
RA de Vries R.P., Record E., Levasseur A., Baker S.E., Bartholomew K.A.,
RA Coutinho P.M., Erdmann S., Fowler T.J., Gathman A.C., Lombard V.,
RA Henrissat B., Knabe N., Kuees U., Lilly W.W., Lindquist E., Lucas S.,
RA Magnuson J.K., Piumi F., Raudaskoski M., Salamov A., Schmutz J.,
RA Schwarze F.W.M.R., vanKuyk P.A., Horton J.S., Grigoriev I.V.,
RA Woesten H.A.B.;
RT "Genome sequence of the model mushroom Schizophyllum commune.";
RL Nat. Biotechnol. 28:957-963(2010).
CC -!- FUNCTION: After transfer of sugars to endogenous macromolecular
CC acceptors, the enzyme converts nucleoside diphosphates to nucleoside
CC monophosphates which in turn exit the Golgi lumen in a coupled
CC antiporter reaction, allowing entry of additional nucleotide sugar from
CC the cytosol. {ECO:0000256|ARBA:ARBA00037742}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}.
CC -!- SIMILARITY: Belongs to the GDA1/CD39 NTPase family.
CC {ECO:0000256|ARBA:ARBA00009283, ECO:0000256|RuleBase:RU003833}.
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DR EMBL; GL377308; EFI95582.1; -; Genomic_DNA.
DR RefSeq; XP_003030485.1; XM_003030439.1.
DR AlphaFoldDB; D8Q936; -.
DR STRING; 578458.D8Q936; -.
DR GeneID; 9588051; -.
DR KEGG; scm:SCHCO_02632240; -.
DR VEuPathDB; FungiDB:SCHCODRAFT_02632240; -.
DR eggNOG; KOG1385; Eukaryota.
DR HOGENOM; CLU_010246_4_2_1; -.
DR InParanoid; D8Q936; -.
DR OMA; WTCRIKE; -.
DR OrthoDB; 180318at2759; -.
DR Proteomes; UP000007431; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0017110; F:nucleoside diphosphate phosphatase activity; IEA:UniProt.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.30.420.150; Exopolyphosphatase. Domain 2; 1.
DR InterPro; IPR000407; GDA1_CD39_NTPase.
DR PANTHER; PTHR11782; ADENOSINE/GUANOSINE DIPHOSPHATASE; 1.
DR PANTHER; PTHR11782:SF83; NTPASE, ISOFORM F; 1.
DR Pfam; PF01150; GDA1_CD39; 1.
DR PROSITE; PS01238; GDA1_CD39_NTPASE; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Hydrolase {ECO:0000256|RuleBase:RU003833};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000007431};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 57..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..98
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 244
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-1"
FT BINDING 277..281
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-2"
SQ SEQUENCE 546 AA; 60195 MW; 1D9DEEEEBF7BBF7D CRC64;
MGPSRGARRG GAAWKKYAIG AAAVIGLIVL FGPRPSPRYL YSDDTDVDTV TDDPLFTDPA
EPIVHNPVIE EEETTRPTRP SDEKPTLKPG EHPTSFETDP DPLHTTYCTT TTADRIVQYA
LMIDAGSTGS RIHVYKFNNC NPNSGPSFEY EVFKMTQPGL SSFAGRPEEA AKSLDVLMDE
AMRVVPESLR KCTPVAVKAT AGLRLLPGSQ SADILDAVRQ RLETAYPFPI HGEDGIVIMD
GKDEGVYAWI TANYLLNTIR PDTPKDTPTF AVLDLGGAST QIVFEPKFPN GAEMADGDHK
YELKFGGKDH VLYQHSYLGY GLMRARRHVH QLIEFMDSLR GKNGGGTFIE KDRRVSNPCL
AQGTQRSVEV EDERNGVTRS VIMDGEGIGS FESCSRTMQL VMAKDAICEL KPCSFDGVYQ
PSLLDAFSDG KVLLLSYFYD RIHPLIPEDK SKVTVSSIKD LASTVCMGRS AWLDRWGAQP
DVMKELEDRP EWCLDLTFMH SLLTLGYEFT EERDVQLGKQ IEGTELGWCL GAALGVVSGA
ELKCKA
//
