ID D8QFJ6_SCHCM Unreviewed; 296 AA.
AC D8QFJ6;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=tRNA pseudouridine synthase {ECO:0000256|RuleBase:RU003792};
DE EC=5.4.99.12 {ECO:0000256|RuleBase:RU003792};
DE Flags: Fragment;
GN ORFNames=SCHCODRAFT_42694 {ECO:0000313|EMBL:EFI93424.1};
OS Schizophyllum commune (strain H4-8 / FGSC 9210) (Split gill fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Schizophyllaceae; Schizophyllum.
OX NCBI_TaxID=578458 {ECO:0000313|Proteomes:UP000007431};
RN [1] {ECO:0000313|EMBL:EFI93424.1, ECO:0000313|Proteomes:UP000007431}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H4-8 / FGSC 9210 {ECO:0000313|Proteomes:UP000007431};
RX PubMed=20622885; DOI=10.1038/nbt.1643;
RA Ohm R.A., de Jong J.F., Lugones L.G., Aerts A., Kothe E., Stajich J.E.,
RA de Vries R.P., Record E., Levasseur A., Baker S.E., Bartholomew K.A.,
RA Coutinho P.M., Erdmann S., Fowler T.J., Gathman A.C., Lombard V.,
RA Henrissat B., Knabe N., Kuees U., Lilly W.W., Lindquist E., Lucas S.,
RA Magnuson J.K., Piumi F., Raudaskoski M., Salamov A., Schmutz J.,
RA Schwarze F.W.M.R., vanKuyk P.A., Horton J.S., Grigoriev I.V.,
RA Woesten H.A.B.;
RT "Genome sequence of the model mushroom Schizophyllum commune.";
RL Nat. Biotechnol. 28:957-963(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC Evidence={ECO:0000256|RuleBase:RU003792};
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375, ECO:0000256|RuleBase:RU003792}.
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DR EMBL; GL377311; EFI93424.1; -; Genomic_DNA.
DR RefSeq; XP_003028327.1; XM_003028281.1.
DR AlphaFoldDB; D8QFJ6; -.
DR STRING; 578458.D8QFJ6; -.
DR GeneID; 9592097; -.
DR KEGG; scm:SCHCO_01133390; -.
DR VEuPathDB; FungiDB:SCHCODRAFT_01133390; -.
DR eggNOG; KOG2554; Eukaryota.
DR HOGENOM; CLU_014673_2_1_1; -.
DR InParanoid; D8QFJ6; -.
DR OMA; YFGWEYN; -.
DR OrthoDB; 167554at2759; -.
DR Proteomes; UP000007431; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF5; TRNA PSEUDOURIDINE(38_39) SYNTHASE; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003792};
KW Reference proteome {ECO:0000313|Proteomes:UP000007431};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694,
KW ECO:0000256|RuleBase:RU003792}.
FT DOMAIN 186..296
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 18..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 106
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001430-1"
FT BINDING 169
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001430-2"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EFI93424.1"
FT NON_TER 296
FT /evidence="ECO:0000313|EMBL:EFI93424.1"
SQ SEQUENCE 296 AA; 33134 MW; BD1F35A0E3231072 CRC64;
DYDTWTKEEL ISRLVQLEGH GSSKPSSKGK GRTQDDTIDW ANAPRRKIAL KFCYGGWDYG
GLAIQKDRTP LPTVEGKLFG ALVRARLVDP KGGLEGCGWE RCGRTDRGVS AAGQVISLWV
RSALSQEELL PHPFTYMINR HLPDPIKILA WSPVDLSFSS RYSCTWRHYK YFFPPVGLDI
PAMRAACAYL PGTHEFRNLC KIDPTKQLDQ FRRDILHASI SHVDASNPGG MHVFDLKGSS
FLYHMVRNIM GVLLSIGARL EPPTLLPSLM NVAPGCRPEY MMADALPLTL WDCGYP
//
