ID D8QNT8_SELML Unreviewed; 1707 AA.
AC D8QNT8;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=SELMODRAFT_437676 {ECO:0000313|EMBL:EFJ38164.1};
OS Selaginella moellendorffii (Spikemoss).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Lycopodiopsida; Selaginellales; Selaginellaceae; Selaginella.
OX NCBI_TaxID=88036 {ECO:0000313|Proteomes:UP000001514};
RN [1] {ECO:0000313|EMBL:EFJ38164.1, ECO:0000313|Proteomes:UP000001514}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21551031; DOI=10.1126/science.1203810;
RA Banks J.A., Nishiyama T., Hasebe M., Bowman J.L., Gribskov M.,
RA dePamphilis C., Albert V.A., Aono N., Aoyama T., Ambrose B.A., Ashton N.W.,
RA Axtell M.J., Barker E., Barker M.S., Bennetzen J.L., Bonawitz N.D.,
RA Chapple C., Cheng C., Correa L.G., Dacre M., DeBarry J., Dreyer I.,
RA Elias M., Engstrom E.M., Estelle M., Feng L., Finet C., Floyd S.K.,
RA Frommer W.B., Fujita T., Gramzow L., Gutensohn M., Harholt J., Hattori M.,
RA Heyl A., Hirai T., Hiwatashi Y., Ishikawa M., Iwata M., Karol K.G.,
RA Koehler B., Kolukisaoglu U., Kubo M., Kurata T., Lalonde S., Li K., Li Y.,
RA Litt A., Lyons E., Manning G., Maruyama T., Michael T.P., Mikami K.,
RA Miyazaki S., Morinaga S., Murata T., Mueller-Roeber B., Nelson D.R.,
RA Obara M., Oguri Y., Olmstead R.G., Onodera N., Petersen B.L., Pils B.,
RA Prigge M., Rensing S.A., Riano-Pachon D.M., Roberts A.W., Sato Y.,
RA Scheller H.V., Schulz B., Schulz C., Shakirov E.V., Shibagaki N.,
RA Shinohara N., Shippen D.E., Soerensen I., Sotooka R., Sugimoto N.,
RA Sugita M., Sumikawa N., Tanurdzic M., Theissen G., Ulvskov P., Wakazuki S.,
RA Weng J.K., Willats W.W., Wipf D., Wolf P.G., Yang L., Zimmer A.D., Zhu Q.,
RA Mitros T., Hellsten U., Loque D., Otillar R., Salamov A., Schmutz J.,
RA Shapiro H., Lindquist E., Lucas S., Rokhsar D., Grigoriev I.V.;
RT "The Selaginella genome identifies genetic changes associated with the
RT evolution of vascular plants.";
RL Science 332:960-963(2011).
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
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DR EMBL; GL377565; EFJ38164.1; -; Genomic_DNA.
DR RefSeq; XP_002960625.1; XM_002960579.1.
DR STRING; 88036.D8QNT8; -.
DR EnsemblPlants; EFJ38164; EFJ38164; SELMODRAFT_437676.
DR Gramene; EFJ38164; EFJ38164; SELMODRAFT_437676.
DR KEGG; smo:SELMODRAFT_437676; -.
DR eggNOG; KOG0433; Eukaryota.
DR HOGENOM; CLU_240710_0_0_1; -.
DR InParanoid; D8QNT8; -.
DR Proteomes; UP000001514; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0032543; P:mitochondrial translation; IBA:GO_Central.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR CDD; cd00010; AAI_LTSS; 1.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 1.10.110.10; Plant lipid-transfer and hydrophobic proteins; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR006458; Ovate_C.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR InterPro; IPR010663; Znf_FPG/IleRS.
DR NCBIfam; TIGR01568; A_thal_3678; 1.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF14368; LTP_2; 1.
DR Pfam; PF04844; Ovate; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF06827; zf-FPG_IleRS; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF47699; Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
DR PROSITE; PS51754; OVATE; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000001514};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..1707
FT /note="isoleucine--tRNA ligase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003121015"
FT DOMAIN 1636..1695
FT /note="OVATE"
FT /evidence="ECO:0000259|PROSITE:PS51754"
FT REGION 1232..1279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1395..1428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1472..1537
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1249..1263
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1482..1503
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1504..1527
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1707 AA; 188967 MW; 7251293DA9E3F904 CRC64;
MLSRIFVTGG IMLLAIAIAT DGAAVDSPPP PSSCDTARAI EALQDCRAAV DAKTGTGKPS
AACCEELKKV GKICLCAIIK EPPRGVEIEK MRKGFVLSVS CEAVGVPAFF FSRGLRERSS
GAMLGLHSFP RSPVLAAAKN AGKQPDSRYK GTIELPQTKF SLKANSVTRE PEIQRFWEQK
QLLETITQKN QGEPFTLHDG PPYANGDLHM GHALNKILKD FINRYQLLQG RKIFYVPGWD
CHGLPIELKV LQSIDAEARM KLSPIELRKK AAEFALKTVD QQRSSFQRYG VWGSWNSPYL
TLQPAYEAAQ IGVFCQMVLN GYIYRGRKPV HWSPSSGTAL AEAELEYPEG HTSRSMYAVF
KMTFASDKFP KELEYLLSSL GVAIWTTTPW TIPGNTGVAV NDKLMYSVVE VVFNNDDSSL
GGKKSTKAGR KYGNGLLKPV VSHLIVAADL VSSLEDKWNV QLVVKGSCTG SALEFCRYKH
PLENREGVIV IGGDYITTDS GTGLVHTAPG HGQEDFVTGQ KYGLPMLSPV DDNGNFTDEA
NEFAGMSVLS DGNEAVIKAL DANSSLLMEE AYGKNNLQMF PRIDSRIVHK YPYDWRTKKP
TIFRATEQWF ASVEGFMPLA LAAIKNVDWI PSQGENRITS MVSGRKEWCI SRQRAWGVPI
PVFYHVDTGE PLLNEQTISH VQAIIAQQGS DAWWSLTTED LLPDTYKAKA SNYRKGTDTM
DVWFDSGTSW ASVLGTKRWP ADVYLEGSDQ HRGWFQSSLL TSVATKGVAP YRQVLTHGFV
LDEKNVKMSK SLGNVVDPRS VIEGGKNQKE QPAYGADVLR LWVASVDYTG DVAIGPQILK
QTADVYRKLR GTLRYLLSNL HDWKPELEIE YDDLPGIDKY TLFQLASVMD SLKDSLDKYQ
FGKFFQSVQR YAVLDLSNFY LDISKDRLYV GGTDSFTRRS CQAVLASLLL YLARGISPVV
PHLAEDAWQH LPFSYILPDG SKAESVFEAG WPEVDPRWRT ISDRDKKLWS LLLELRVEVN
KVLESARMGK LIGANLDAKV CLHLGGEDDA LLHSTLQAMA GSEDGTVDKL HVIFLTSQVE
VVDCEDADCA YTGSCKLEGS GGRAWIGVKR AEGQKCERCW KYSPAVGSFT LHSTLCERCY
KIRRGRGEED EQLGAKGNEE QGRGVESSID ACVWTFVGSP NLEWHCRRVD CAPHFTGDGS
RAMGRRRLRF KFTNVISRVF LLRAHRVKPV APSSVVAPAA SKKSSSSFTS DSNREEEPEE
ERSLIAPAPA APAPAKAKKK NRKPVFGYSC CSREEAIVRE NCQYKRVKGL SKKPVGAASS
RAAPMVVASR QEEEMWEREG LWSVIAAVDS GKVVKKKVVL KKRKKSQSSP RLLRAPANSE
ISAMEEQVLI VGQAARVTSD PSSSERERKG SSTHTFRLST SSGDASLGPE ALAWDKEHSL
FASDDSSAPG SSKLVHRATT LEDEQRHEAI EEVGLKNHAG QTPRPKKSSS SSSASTSGSS
GGGTRGHHHR HHLHHHHHHH HHNSSFHLSR NHASGNKMTG ALSEFDELGL LLSSSSKMPP
PPPRCDGKRI SSASSLESSA KFWEDGREVA SKVLPEFPRH KVGAESAPAL DDHARTKKKK
KPKDLVRGKV RESVPVMLVS SNPYEDFRQS MVQMIFEKRL NKAADLEELL ECYLYLNPPG
FHEVIVQVFT DLWLQALSTE NQQQQNI
//
