ID D8U864_VOLCA Unreviewed; 1817 AA.
AC D8U864;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 67.
DE RecName: Full=Eukaryotic translation initiation factor 5B {ECO:0000256|ARBA:ARBA00013824};
DE AltName: Full=Translation initiation factor IF-2 {ECO:0000256|ARBA:ARBA00032478};
GN Name=eif5B {ECO:0000313|EMBL:EFJ44086.1};
GN ORFNames=VOLCADRAFT_121350 {ECO:0000313|EMBL:EFJ44086.1};
OS Volvox carteri f. nagariensis.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Volvocaceae; Volvox.
OX NCBI_TaxID=3068 {ECO:0000313|Proteomes:UP000001058};
RN [1] {ECO:0000313|EMBL:EFJ44086.1, ECO:0000313|Proteomes:UP000001058}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=f. Nagariensis / Eve {ECO:0000313|Proteomes:UP000001058};
RX PubMed=20616280; DOI=10.1126/science.1188800;
RA Prochnik S.E., Umen J., Nedelcu A.M., Hallmann A., Miller S.M., Nishii I.,
RA Ferris P., Kuo A., Mitros T., Fritz-Laylin L.K., Hellsten U., Chapman J.,
RA Simakov O., Rensing S.A., Terry A., Pangilinan J., Kapitonov V., Jurka J.,
RA Salamov A., Shapiro H., Schmutz J., Grimwood J., Lindquist E., Lucas S.,
RA Grigoriev I.V., Schmitt R., Kirk D., Rokhsar D.S.;
RT "Genomic analysis of organismal complexity in the multicellular green alga
RT Volvox carteri.";
RL Science 329:223-226(2010).
CC -!- FUNCTION: Protein transport. Probably involved in vesicular traffic.
CC {ECO:0000256|ARBA:ARBA00025673}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000256|ARBA:ARBA00007733}.
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DR EMBL; GL378367; EFJ44086.1; -; Genomic_DNA.
DR RefSeq; XP_002954887.1; XM_002954841.1.
DR STRING; 3068.D8U864; -.
DR GeneID; 9621563; -.
DR KEGG; vcn:VOLCADRAFT_121350; -.
DR eggNOG; KOG1144; Eukaryota.
DR InParanoid; D8U864; -.
DR OrthoDB; 169393at2759; -.
DR Proteomes; UP000001058; Unassembled WGS sequence.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR CDD; cd03703; aeIF5B_II; 1.
DR CDD; cd01887; IF2_eIF5B; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR002547; tRNA-bd_dom.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43381:SF4; EUKARYOTIC TRANSLATION INITIATION FACTOR 5B; 1.
DR PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS50886; TRBD; 1.
PE 3: Inferred from homology;
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540,
KW ECO:0000313|EMBL:EFJ44086.1};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000001058};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00209};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00209}.
FT DOMAIN 1..77
FT /note="TRNA-binding"
FT /evidence="ECO:0000259|PROSITE:PS50886"
FT DOMAIN 975..1205
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT REGION 95..389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..669
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 683..745
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 759..951
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..168
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..208
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 278..299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..348
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..474
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..519
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 573..669
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 708..724
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 791..848
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 886..943
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1817 AA; 194863 MW; B32223387ADB9E05 CRC64;
MSVVVTNAAN VEEGMKVVFA PVGSTTPGSG LKMERKQLKG VESFGMLCSA YDIGWIPEPD
NILVVLPDNA VVGEPCPKEA PKGLKSKLLS SAVDGEEAEE EEEAAAAVAA KPKKDAKGGK
KGKKGGKDMS SLFAALEEDA AEAVEEEEVE PVPKPKSGKD KKAKRGGKDM SSLFATLEED
AEGGGSKAEE EEAEEPAPKP KASKGKKGGK DMSSLFAALE EDAAGGAKEP EEEEEEEPAP
KPKASKSKKG GKDMSSLFAA LEEDAAASTS TAVAEDDGDA AAPKKKDGKK SKKTGKDIDS
LLAALEGDAA TSSAPAAEGP AAAAQDDGDA PKQKKEKKKK KDMSDLFAAL EADAEASSTP
APAVPEKPSP AAAGKGGKAA AKNKEEEEVE ALLAELDKPK EPAAVDAAIT SERVSAMAVL
PKAHQESGFL LCLIVLKQEL NCAIWHPTGK KNDKKKKGKG GKAEKEDEDI DALLAMIDGP
KQQQAKPVEV TAPAPAVAEP VAADGEGGEG EEGADEAGGE EGGEGGKELT AAQKKKLKKK
QKEKEKKAAA TEGGAPAAEG AAAPAEVAAE EEAATGGKGK KGKPVKESAI AKKLREQLEA
RKKAEEEAAR LEAERRAREE EELRRLEEEE RRKEEEKQRK KEAQRLKREQ LKREGKLLTG
KAKEEAERRA AMAAQLLAKA AEKGIDLSQP AEAEKKKKVV YSSKKKLGKK APGQEGDREG
SPEAPAEAEA AASPKEAAAA AAPAEAAVLA EATVAAVAEE PAAAAGPAAA AAPEPEPEAL
VDDWDAVDDW DKIDPDALLA SKKTKEQEEA EARAAAEAEA KAKAEAKAKA EAEAKAKAEA
EAKAKAEAEA AKAAAAAKAT ANGKAAVKPV KEAPKAAAKP KDVDEDESAS EEESEEDEES
GSESGSEESG SEEESSEEES SEEESSDESD DDDEDDSDDY SEDSEEARQQ RIDEARERRL
ARAAEARKNA NKEQLRSPIC CILGHVDVGK TKILDNIRRT NVQDGEAGGI TQQIGATFVP
AEAVERRTET LRGGRAFDMK LPGLLVIDTP GHESFTNLRQ RGSGLCDMAV LIVDLVHGLE
QQTIESINLL KMRKTPFVIA LNKASRCRHN CRSNRDVDRL YGWKSVPDSP IRDSFKRQKE
FVMSEFEQRC SQVFLQLNEQ GLNVSLYWKN SDPRKFVNVV PTSAITGEGI PDLLQLIVKL
TQTMMTDRLM FVADTQCTVL EVKTMEGLGT TVDVVLVNGV LREGDKIVVC GLGGPIVTRI
KALKTPQVLR ELRVKGALME HKEIRAAMGV KIVAPGLESA VAGTSLFVVS PDDDEEELKD
AVMEDMADIF SKVDKGGEGV CVQASTLGSL EALLAFLSSP EVNIPVSGIN IGPVHKRDVL
RANVMNEKKC PKYAVILAFD VPVSKEARDL AEDYNVKVFT ADIIYHLFDQ FTAYVKQVKS
AEQEAARFKA VFPVVLRILP TCVFNKKDPI VVGVDIAEGI AKIGTPVAAQ TEAGLVELGR
IAGMEINHKA VEKARAGESV AMKIEGQTTE EKSRLFGRHF DHTHQLVSVI SRESIDALKE
YFADEMTKDD WRLVIKLKKV FQSRHSPRLA FSGVHVHEHN RRGVHTHRAR LVSSIYLRSR
YCHCKPLISL LHFVLISAMA DQVLPGNIPN NNNQNNPNNV VIQPPAAAQG VPAQVVEQLV
QDQNFNAVFT NISAGREEAL SRRYTDLEQN HEASCPKAFK FMAAWIRDIG SKVYSAHSAL
KVIQATVADV GPIVDILQQS VFIMDLLYAS CDAGGRCHQF HLEVMQQYFN NNRHQAVSPT
AEVKADTNRL YDCGCGI
//
