ID PFKB_THETC Reviewed; 322 AA.
AC D9TT10;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=ATP-dependent 6-phosphofructokinase {ECO:0000305|PubMed:24599311};
DE Short=ATP-PFK {ECO:0000305|PubMed:24599311};
DE Short=Phosphofructokinase {ECO:0000303|PubMed:24599311};
DE EC=2.7.1.11 {ECO:0000269|PubMed:24599311};
GN Name=pfkB; OrderedLocusNames=Tthe_1922 {ECO:0000312|EMBL:ADL69408.1};
OS Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 /
OS NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) (Clostridium
OS thermosaccharolyticum).
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Thermoanaerobacterium.
OX NCBI_TaxID=580327;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM
RC 00135 / 2032;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA Hemme C.L., Woyke T.;
RT "Complete sequence of Thermoanaerobacterium thermosaccharolyticum DSM
RT 571.";
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBSTRATE SPECIFICITY.
RX PubMed=24599311; DOI=10.1007/s00253-014-5621-y;
RA Verhaeghe T., Aerts D., Diricks M., Soetaert W., Desmet T.;
RT "The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-
RT phosphate phosphorylase as a novel specificity.";
RL Appl. Microbiol. Biotechnol. 98:7027-7037(2014).
CC -!- FUNCTION: Catalyzes the ATP-dependent phosphorylation of D-fructose 6-
CC phosphate to fructose 1,6-bisphosphate. Together with the adjacently
CC encoded sucrose 6'-phosphate phosphorylase, may be involved in a new
CC pathway for the degradation of sucrose. Cannot phosphorylate D-
CC fructose. {ECO:0000269|PubMed:24599311}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-
CC bisphosphate + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634,
CC ChEBI:CHEBI:456216; EC=2.7.1.11;
CC Evidence={ECO:0000269|PubMed:24599311};
CC -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC {ECO:0000305}.
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DR EMBL; CP002171; ADL69408.1; -; Genomic_DNA.
DR RefSeq; WP_013298374.1; NC_014410.1.
DR AlphaFoldDB; D9TT10; -.
DR SMR; D9TT10; -.
DR STRING; 580327.Tthe_1922; -.
DR KEGG; ttm:Tthe_1922; -.
DR eggNOG; COG0524; Bacteria.
DR HOGENOM; CLU_027634_6_0_9; -.
DR OrthoDB; 9788681at2; -.
DR Proteomes; UP000001626; Chromosome.
DR GO; GO:0003872; F:6-phosphofructokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IEA:UniProt.
DR GO; GO:0006000; P:fructose metabolic process; IEA:UniProt.
DR CDD; cd01167; bac_FRK; 1.
DR Gene3D; 3.40.1190.30; -; 1.
DR Gene3D; 3.40.1620.20; -; 1.
DR Gene3D; 6.10.140.490; -; 1.
DR InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR InterPro; IPR011611; PfkB_dom.
DR InterPro; IPR002139; Ribo/fructo_kinase.
DR InterPro; IPR029056; Ribokinase-like.
DR PANTHER; PTHR43085; HEXOKINASE FAMILY MEMBER; 1.
DR PANTHER; PTHR43085:SF1; KETOHEXOKINASE; 1.
DR Pfam; PF00294; PfkB; 1.
DR PRINTS; PR00990; RIBOKINASE.
DR SUPFAM; SSF53613; Ribokinase-like; 1.
DR PROSITE; PS00583; PFKB_KINASES_1; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..322
FT /note="ATP-dependent 6-phosphofructokinase"
FT /id="PRO_0000442436"
FT BINDING 201
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O59128"
FT BINDING 234
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O59128"
FT BINDING 239
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:O59128"
SQ SEQUENCE 322 AA; 36535 MW; A57BE1B25FC0ED87 CRC64;
MFNFNDKIVF DDKKYDVLTV GEMLVDMIST DYGDDFECDT YKKYFGGSPA NIAINSKMLG
INSIIVSSVG NDGLGKFLLK KLQEHHIEIK YVRQVDYSTS MVLVTKSKSS PTPIFYRDAD
YHIEYSDELK YLIENTKIVH FSSWPISRNP SRSTVEILID ECKKYDVLVC YDPNYHSMIW
ERGHDGREYI KSLIAKVDII KPSEDDAERI FGKDTPENQL KKFLDLGAKL VILTLGKDGA
IVSNGEETIR FNTLADEVVD TTGAGDAFWS GFYSGLIKGY TLKKSLELGF AVSAYKLRYV
GAIVDLPDID TIKSMYDLKK LR
//
