ID D9WIU8_9ACTN Unreviewed; 4613 AA.
AC D9WIU8;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE SubName: Full=Polyketide synthase {ECO:0000313|EMBL:EFL27601.1};
GN ORFNames=SSOG_07315 {ECO:0000313|EMBL:EFL27601.1};
OS Streptomyces himastatinicus ATCC 53653.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
OX NCBI_TaxID=457427 {ECO:0000313|EMBL:EFL27601.1, ECO:0000313|Proteomes:UP000003963};
RN [1] {ECO:0000313|EMBL:EFL27601.1, ECO:0000313|Proteomes:UP000003963}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 53653 {ECO:0000313|EMBL:EFL27601.1,
RC ECO:0000313|Proteomes:UP000003963};
RG The Broad Institute Genome Sequencing Platform;
RG Broad Institute Microbial Sequencing Center;
RA Fischbach M., Godfrey P., Ward D., Young S., Zeng Q., Koehrsen M.,
RA Alvarado L., Berlin A.M., Bochicchio J., Borenstein D., Chapman S.B.,
RA Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA Gujja S., Heilman E.R., Heiman D.I., Hepburn T.A., Howarth C., Jen D.,
RA Larson L., Lewis B., Mehta T., Park D., Pearson M., Richards J.,
RA Roberts A., Saif S., Shea T.D., Shenoy N., Sisk P., Stolte C., Sykes S.N.,
RA Thomson T., Walk T., White J., Yandava C., Straight P., Clardy J., Hung D.,
RA Kolter R., Mekalanos J., Walker S., Walsh C.T., Wieland-Brown L.C.,
RA Haas B., Nusbaum C., Birren B.;
RT "Annotation of Streptomyces hygroscopicus strain ATCC 53653.";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; GG657754; EFL27601.1; -; Genomic_DNA.
DR STRING; 457427.SSOG_07315; -.
DR HOGENOM; CLU_000022_8_0_11; -.
DR Proteomes; UP000003963; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0009403; P:toxin biosynthetic process; IEA:UniProt.
DR CDD; cd12116; A_NRPS_Ta1_like; 1.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd08953; KR_2_SDR_x; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 1.10.1240.100; -; 2.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 3.40.50.980; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 4.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 4.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 306..382
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1381..1462
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1493..1918
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2841..2917
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2959..3386
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4041..4122
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4167..4571
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT REGION 285..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1473..1496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4011..4041
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4613 AA; 490069 MW; 168BA3F1B0024E45 CRC64;
MAGAHQAEGA DRWEAEFERV ADACPDIEPT VTLNREFLRA YPRLLRGELV GTEVMFPDSS
MELVQDLYRG NPLTDFFNLL AADTVRAFLG ARLPQLPAHE SVQVIELGAG TGATSERVLS
ALVEHSGRVG FTFTDISPRF LEHGRERFAA AYPFAGFQLL NLERGLAEQD FVPGSADVVL
ATNVVHATRN LRTTLRKVRE LLKPGGLLVL NELTAIRPYL TVGGGVMEGW WAFEDSELRI
PGSPLATPPA WTRLLQEEGY ARVRVLGAEE GEWGQRLLVA ESDAAVPGGP SAEPAAEVAT
TGSRPDPADG IEDRLRELLE RVLKLDERID PDRPLADYGF DSLSGLKIVA AVDDAFGVPV
PLDEFFERPT LRELTAHLTS GWLTAVVAPA AAAEAAPMTQ PSPVAAAAAP VLRPRTDAEL
ITHPLSEGQR ALWVIEQIAP GNHAYNLPLA FWLDRDVDVL ALRIVLQDVL DRHDALRSTV
RTGEDGRPFV RIAAEPELPF QQVFLTSDDE ADLRDRIRVD MRQPFELSRG PLVRATLYTL
GDGRHALLLT VHHLVLDGVS IPLLLDDVER GYRTVRQGRP LPDSRPTRTF ADFTAHQRDL
LAGPEGERLR AYWTDRLRDR RTTVPLPLDR PRPPVPGFRG AVVEGRLPAA VTEGARALAT
AERTSLSSVM LAAFFALLHR YSDQADIAVG TPTANRPAEG YDDVLGYFMN MVVLAERVDG
QEPFHTLVRR VHRTVMDALR HGAYPLITLA EELRREHPAA HDRLFDVAFY FHNWARDMRN
DGLVRDRVEG VHQEGEFDLT LDLIDHPEGC RYTLKYDPDL FDAATVERIG GHFGTLLASA
VGAPGATVGD LELLTEGERA MLAPPATAAY PADTLVWELV RRQAEARPDA VAVRFQGTEL
TYRRLRERVD ALAARLVAAG VGRGRTVGVL LPRDAGLPVA LLAVQAAGGA YVPLDPAYPA
QRLAYMAEDA GLHLLLTHSA VEATCGDTAP RLLVDGDGQP PAAASPGPAG PLDTAYVLYT
SGSTGRPKGV RVPHRALVNF LWSMLREPGF GAGDTLLALT TVCFDISGLE LYLPLISGGT
VEIVPTEVAQ DGLLLREEVE RGTATVIQAT PATWKMLLAA GWQGNPDLRV FCGGEALPYD
TAQALLATCR EVWNLYGPTE TTIWSAVSRL RRGEPVTIGT PVANTGLYVL DSAHRPVPTG
VAGELYIGGD GVADGYLGRP ELTAERFLPD PFATRPDATM YRTGDLVRRL ADGRIAYLGR
MDAQVKVRGF RVEPEEIESA LRRLEGVREA AVVAHGRGGG DSTLLACYVL DDGAPEPARD
RLAAWLPSHM LPDALIRMAS LPYTLNGKVD RTALATLAPA DLRARFGNPA TERPPRSAEP
AAATGRVAAL ISELALMTGE LADVRPQDIG PETPLGEVGM NSVRFTALSA ELRNAYGIEV
RPTLFYRKGT LSALAEHLWE HHPAELTSRF GAAAERPAAE PRPAGQPAGP RPGGDAAIIG
MAGRLPGSEN LDAFWSHLAA GHDLVGEVPA ERWDWRDQPS SRWGGFIPGV DRFDAAFFGI
SPREAELMDP QQRLLLEVVW AAVEDAGYRP SDLAGQRVGV FIGVANADYL EAQRAAGHAP
QSHTATGAAL SVIPNRISYL LDLRGPSMAV DTACSGSLSA VHLACAALRD GTCDLALAGG
VNLILTPTVY EVLSQGEMLS PDGRCKTFDS RADGYVRGEG VGAVLLKLDD RARRDGDAVH
AVIRAAAAGH GGRTTSLTAP SPDAQADLLA EAYRTAGVPL ETVGYIETHG TGTALGDPIE
TTGLSTAFRR LRAEQGTTAA PGCAIGSVKT NIGHLEAAAG IAGLFKVVLA MRHGTIPASL
HLREQNPYLE LDGGPFEVAA TARPWPRPRD AAGHELPRRA GVSSFGFGGA NAHVVVEEDH
MPDTREDGAV QQLFVLSARD PEALRRSARQ LADHLADHDV RPGDLAYTLQ TGREPMGHRL
AVVADGTAGL RAELVAHLSG HPSAVRTGRA ERGATPHPAV SDTPDVATVS RAWLSGADID
WRSLHRGAAP RRVHLPTYPF AATRHWFAPG PSPAVAPGRA VTPPRPALLD ADVPTEGGPA
FAKTLTGEEF FLRDHVVGGE PVLPGVVYLE MARLAGERAL GAPVRGVDGL VWASPIRVPA
GSEHRVVVRL TGDGGTSFEV CTAGDDVTVH AHGTLLVGEA GDRPARIDPA GVRARCTAVR
AGADCYTAFS RLGLDYGPSF QVIEELAVGE REALASLRLP GARVADAADY VFHPSLLDAA
LQTAGRLAPG ADDLTRPLPY LPFSLGSVRL YGELPELMYA YATPSARPAT AGTLCFDVAL
LDRAGDVVLA IESFTLRAVP AGHMVAARGT AAADDAVAAF EPVREPAPLA TRQWQGTVLV
LDAGSHGPAV GAALTAAGAT VQHIELTAGF DAGAAVRAGG QGPGPVHVVH LAGEDADPGS
AVEHGFHVAL AFCREWEHER RDALRYLYVH RAPAVSPAHP AMAAFARSVR SEHPSLDFGV
LAVADGSLAE AVAGELGAEG GPEVRIDATG RTRGSWQRVS LPAATGSAFT GSGAHVITGG
TGALGLLLAE HIAAARRAAG VTEGGIVLAS RTAPDPGTEA RARAIGARVV QADGSDAAAV
HALIAGARRE HGAVSGVVNA AGVLRDALLR HKSRADAEEV LAAKVHGTAL LDEATREDAL
DYFVTFSSAA AAFGNVGQTD YAFANAFLDH FAERREEQRR RGTRHGRTLA AAWPLWADGG
MRVDSSGQEH AARVLGMRPL RTRPALDALE RALAGTAPRL LLGAGDPARI LTALRGEEKT
KHTTNPTAPG AQAAEVADTT DVRARAERLV LRIMAEELKL PEAEIAVTET FDQYGVDSLI
TLSVTRRLEE HVGPLSKTLL FEYVTVRELA DHLAGAHPEA LATRHPAADP ATADPAAVEA
VATGAAPADP ATAHTTPRGQ DIAIIGVAGR YPQADDVDEF WRNLREGRDC VEEIPSERWN
NDLFHDADTS RTGKTYGRWG GFIRGADRFD PMFFRMSHIE AEHTDPQERV FLETVWHLLE
DAGVTREGLR GFRTGVFVGM MYGQYQLYGV QEALRGEGLP PHSSFASVAN RVSYFFDFTG
PSVAVDTMCS SALVTIHQAC QAIRDGDCDV AVAGGVNITS HPAKYLQLAW RGFLAEDGRC
RSFGEGGTGY VPAEGSGAVL LKRLDAALAD GDRILAVVKA SAVNHGGTGR GFNVPSPRAQ
GDLVRAALDR AGMRPADLDY VEAHGTGTAL GDPVEVTGMM RAFGDDMPER LPIGSVKSAI
GHAESAAGIA AVTKVLLQMR HGELVPTLHA DPLNPNIDFA ATPFEVQRAL APWPRRPLAD
GTTRPRTAGV SSFGAGGTNA HVILQEYPAV ALPPARPAGP HLAVLSAREP DRLAEQARRL
AAHLRAEGSG AAPQEVAWTL QSGREAMAHR LAVRFDDLPQ LATRLEEFAA GGEPADCWTG
TVDPRKPADD ATPSTDPTAC AVAWAAGRRV DWAALHGEAR PGRVPLPGYP FGGGRVWLAA
ADAELAALET GPTAGGLLLT RQWMPAEPAR RTMPPGRTAV LAVPGTEGPA ARLAATLPTA
EILTRERLRA DLDASDTRWD RYDAVVDLAG CAPAGEPVDR SVLHTWLGWL QRVVDGGGRG
LSALLVSRGT TPAPAGAART GLYRMLQSEY RHLRSRHLDL GQADDEQLCR WVADELGAGD
EPPEVAYRDG IRHRTVLREL PDRTGEPVRF PAGHVLWVTG GTRGIGLLTA RHFVARYGVR
KLVLTGREEL PPRADWAAHI AEGGVLGRKL RPLAELADQG VELEVLSVPL DDRQAMATAL
ADVTRRLGPA GGLIHSAGVT DTANPAFVRK PRTGIEQVIG PKVFGLDTLV ECFRDQPLAL
FVLYSSVAAT VPALAVGQSD YAMANAYLDA VAEARPHGLP LVSVQWPSWK DTGMGEARSA
AYRASGLATL PDEQGLALLD RALASGARVV MPAVVRPDAD WQPERLTGRH LAPAPVPTEA
PAAPPEPSGA HPGTAEGAAQ GAVQAAMDAA ASWLLDQLAV ELRFDRARLS GDVPVHDYGI
DSILVTQLVQ TLAKQLDVSI DPSALLEHPT ADGFAAHLAR EHRDELVARF GGAPSDAPLP
AAYVAQKEAV ADPVSAVSVS AEPVPAGADI AVVGLSCLFP GAGSAGEYWE LLREGRSALR
PVPEGRFGGS AGHLAGLLPD VLGFDPEAFL LSEADVAAMD PQALLLLEQV NSAVHDAGYR
PAELKGRRVG VYVGARSGWA PDDIRLGQAK NPVMATGQNY LSANVSQFFD FRGPSMVVDT
ACSSALVAMD MAVRALRAGD IESAVVAGVS LLMDGRAHEV FARRGLLNPG SEFHLFDRRA
AGLVLGEGAG AVLLKPLARA QSDGDRVLAV LKGIAVNNDG RTAGPATPNP QAQKEVMAQA
LAHSGHLPGD IGWVEANGSG STVTDLLELK AIQAVYRSGS AQPVALGSVK PNIGHPLAAE
GIAAFIKVVL MLRHQEQVPF RSGQEPPAHF DLAASPLYFP REPRPWPGTA GIAALNCFAD
GGTNAHLLVG PAPVGHHPAR RPLPEAALNR RVVIRGAAAP AAPAGGLFWD TYR
//
