ID DAMT1_CAEEL Reviewed; 365 AA.
AC Q09956;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 128.
DE RecName: Full=DNA N6-methyl methyltransferase {ECO:0000305};
DE EC=2.1.1.72 {ECO:0000269|PubMed:25936839};
DE AltName: Full=DNA N6 adenine methyltransferase 1 {ECO:0000303|PubMed:25936839};
GN Name=damt-1 {ECO:0000303|PubMed:25936839};
GN ORFNames=C18A3.1 {ECO:0000312|WormBase:C18A3.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF 191-ASP--TRP-194.
RX PubMed=25936839; DOI=10.1016/j.cell.2015.04.005;
RA Greer E.L., Blanco M.A., Gu L., Sendinc E., Liu J.,
RA Aristizabal-Corrales D., Hsu C.H., Aravind L., He C., Shi Y.;
RT "DNA Methylation on N(6)-Adenine in C. elegans.";
RL Cell 161:868-878(2015).
CC -!- FUNCTION: Methylates DNA on the 6th position of adenine (N(6)-
CC methyladenosine) (PubMed:25936839). N(6)-methyladenosine (m6A) DNA is
CC involved in epigenetic transgenerational inheritance (PubMed:25936839).
CC {ECO:0000269|PubMed:25936839}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000269|PubMed:25936839};
CC -!- SIMILARITY: Belongs to the MT-A70-like family. {ECO:0000255|PROSITE-
CC ProRule:PRU00489}.
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DR EMBL; BX284602; CCD65009.1; -; Genomic_DNA.
DR PIR; T15545; T15545.
DR RefSeq; NP_495127.1; NM_062726.1.
DR AlphaFoldDB; Q09956; -.
DR SMR; Q09956; -.
DR STRING; 6239.C18A3.1.1; -.
DR PaxDb; 6239-C18A3-1; -.
DR PeptideAtlas; Q09956; -.
DR EnsemblMetazoa; C18A3.1.1; C18A3.1.1; WBGene00015939.
DR GeneID; 173970; -.
DR KEGG; cel:CELE_C18A3.1; -.
DR UCSC; C18A3.1; c. elegans.
DR AGR; WB:WBGene00015939; -.
DR WormBase; C18A3.1; CE01793; WBGene00015939; damt-1.
DR eggNOG; KOG2356; Eukaryota.
DR GeneTree; ENSGT00390000016237; -.
DR HOGENOM; CLU_027091_0_1_1; -.
DR InParanoid; Q09956; -.
DR OMA; WRWLKVT; -.
DR OrthoDB; 179166at2759; -.
DR PhylomeDB; Q09956; -.
DR PRO; PR:Q09956; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00015939; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IBA:GO_Central.
DR GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IDA:UniProtKB.
DR GO; GO:0040029; P:epigenetic regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0080009; P:mRNA methylation; IBA:GO_Central.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR007757; MT-A70-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12829:SF4; N(6)-ADENINE-SPECIFIC METHYLTRANSFERASE METTL4; 1.
DR PANTHER; PTHR12829; N6-ADENOSINE-METHYLTRANSFERASE; 1.
DR Pfam; PF05063; MT-A70; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51143; MT_A70; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..365
FT /note="DNA N6-methyl methyltransferase"
FT /id="PRO_0000433613"
FT MUTAGEN 191..194
FT /note="DPPW->APPA: Abolishes m6A DNA methylation."
FT /evidence="ECO:0000269|PubMed:25936839"
SQ SEQUENCE 365 AA; 42215 MW; 2659B45E5B438939 CRC64;
MDTEFAILDE EKYYDSVFKE LNLKTRSELY EISSKFMPDS QFEAIKRRGI SNRKRKIKET
SENSNRMEQM ALKIKNVGTE LKIFKKKSIL DNNLKSRKAA ETALNVSIPS ASASSEQIIE
FQKSESLSNL MSNGMINNWV RCSGDKPGII ENSDGTKFYI PPKSTFHVGD VKDIEQYSRA
HDLLFDLIIA DPPWFSKSVK RKRTYQMDEE VLDCLDIPVI LTHDALIAFW ITNRIGIEEE
MIERFDKWGM EVVATWKLLK ITTQGDPVYD FDNQKHKVPF ESLMLAKKKD SMRKFELPEN
FVFASVPMSV HSHKPPLLDL LRHFGIEFTE PLELFARSLL PSTHSVGYEP FLLQSEHVFT
RNISL
//
