ID DEXH4_ARATH Reviewed; 1417 AA.
AC Q9C6G0;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 27-MAR-2024, entry version 153.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH4, chloroplastic {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=At1g58050 {ECO:0000312|Araport:AT1G58050};
GN ORFNames=T15M6.6 {ECO:0000312|EMBL:AAG50700.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
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DR EMBL; AC079604; AAG50700.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33491.1; -; Genomic_DNA.
DR PIR; F96613; F96613.
DR RefSeq; NP_176102.1; NM_104587.1.
DR AlphaFoldDB; Q9C6G0; -.
DR SMR; Q9C6G0; -.
DR STRING; 3702.Q9C6G0; -.
DR iPTMnet; Q9C6G0; -.
DR PaxDb; 3702-AT1G58050-1; -.
DR ProteomicsDB; 224130; -.
DR EnsemblPlants; AT1G58050.1; AT1G58050.1; AT1G58050.
DR GeneID; 842172; -.
DR Gramene; AT1G58050.1; AT1G58050.1; AT1G58050.
DR KEGG; ath:AT1G58050; -.
DR Araport; AT1G58050; -.
DR TAIR; AT1G58050; -.
DR eggNOG; KOG0920; Eukaryota.
DR HOGENOM; CLU_001832_1_4_1; -.
DR InParanoid; Q9C6G0; -.
DR OMA; IMRELCN; -.
DR OrthoDB; 53793at2759; -.
DR PhylomeDB; Q9C6G0; -.
DR PRO; PR:Q9C6G0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6G0; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR CDD; cd17917; DEXHc_RHA-like; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF145; ATP-DEPENDENT RNA HELICASE DHX29; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Chloroplast; Helicase; Hydrolase; Nucleotide-binding; Plastid;
KW Reference proteome; RNA-binding; Transit peptide.
FT TRANSIT 1..61
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 62..1417
FT /note="DExH-box ATP-dependent RNA helicase DExH4,
FT chloroplastic"
FT /id="PRO_0000435294"
FT DOMAIN 607..781
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 868..1043
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 722..725
FT /note="DEIH box"
FT /evidence="ECO:0000305"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 620..627
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1417 AA; 159339 MW; 9AE544B1AD9DB570 CRC64;
MAPTKKPQKN KQSKNEIASS LIPNSGHKKP SKAPKLLISP ENEDRLRRLL LNFRRTPSPV
TATLSVTQKR KKLNNLYENL SCEGFLDNQI ELVLSSLRDG ATLETALDWL CLNLPSHELP
VNFSNGASRF PSTGRSVAVI SKSKKDWNVS AESSVQEVKE VPESEVLVRV KSKRDEEEED
SLSSCQPSQA DWIHQYMKRL EEEELESSDD ERDKVSGPRS FEVIAKEYCV ERYNAIKAKR
KGDKSGQSQA GLAICKLKEE MNALGPSEAI LESEFQRDRQ DCEGAREKEV ASSVLDDVHE
SVNADAFFFQ LFDDLTLDTN PVGSCKSEEQ ESVVSNDSLD DLDLGDLFDE DVPPYVPSPH
ELLELQKKEI MRELCNEKHL TKLNGIWKKG EAQKIPKALL HQLCQRSGWI APKFNKVTGE
GRNFSYTTSV MRKSSGFGKS RQAGGLVTIQ LPHQVEDFES IQDAQNRVAA FALHKLFSDL
PVHFAITEPY ASLVLIWKQE ESLGITSREE QRREKFVESL LEADNFSLTT TSRGIHSALP
MVDSCVKEND DLDVVKSNHR ARRNSSMAAE CSSLKQKQEN KKKMQKYKDM LKTRAALPIS
EVKKDILQKL KEKDVLVVCG ETGSGKTTQV PQFILDDMID SGHGGYCNII CTQPRAITVA
QRVADERCEP PPGFDNSVVA YQVRHQNARS DKTRLLFCTT GILLRKLVGD TTLKDVTHII
VDEVHERSLM GDFLLIILKS LIEKQSWDNA LPKLKVILMS ATVDAHQFSR YFGQCPIITA
QGRTHPVTTY FLEDIYERTK YLLASDSPAA LSSDTSITDK LGSVNVPRGK KNLMLAGWGD
SYLVSEDSLN TSYDSIKYIA SAVVDYDLLE ELICHIDDTC EEGAILVFLP GMSEINMLLN
RLAASYRFRG ASGDWLLPLH SSIASTEQKK VFLRPPKGIR KVIIATNIAE TSITIEDVVY
VIDSGKHKEN RYNPHKKLSS MVEDWVSKAN ARQRMGRAGR VKPGHCFSLY TRHRFEKLMR
PYQVPEMLRV PLVELCLHIK LLGLGQIKPF LSKALEPPSE SAINSAILLL HKVGALEGDE
ELTPLGHHLA KLPVDLLIGK MLLYGGIFGC LSPILSIAAF LSCCKSPFVY AKDEQNVDRV
KLALLSDKLE SSSNLNNNDR QSDHLLMVVA YEKWVRILHE QGFKAAESFC ESKFLNSSVM
RMMRERRVEF GMLLADIGLI NLPKGKGRRK ENFDVWFSDK TQPFNMYSQE PEVVKAILCA
GLCPNIAEGL VNRLTKPAEE TQRYAVWHDG KREVHIHRNS INKNCKAFQY PFIVFLEKLE
TKKVVYLQDT TVVSPFSILL FGGSINVHHQ SGSVTIDGWL KLTAPAQTAV LFKELRLTLH
SILKDLIRKP EKSGIVHNEV VKSMVHLLIE EGKPQHT
//
