ID DNAE2_METCA Reviewed; 1047 AA.
AC Q605W1;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 103.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=MCA2164;
OS Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylococcus.
OX NCBI_TaxID=243233;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX PubMed=15383840; DOI=10.1371/journal.pbio.0020303;
RA Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S.,
RA Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E.,
RA Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.,
RA Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L.,
RA Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H.,
RA Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R.,
RA Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.;
RT "Genomic insights into methanotrophy: the complete genome sequence of
RT Methylococcus capsulatus (Bath).";
RL PLoS Biol. 2:1616-1628(2004).
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR EMBL; AE017282; AAU91620.1; -; Genomic_DNA.
DR RefSeq; WP_010961400.1; NC_002977.6.
DR AlphaFoldDB; Q605W1; -.
DR SMR; Q605W1; -.
DR STRING; 243233.MCA2164; -.
DR KEGG; mca:MCA2164; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_4_0_6; -.
DR Proteomes; UP000006821; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA damage; DNA repair; DNA replication;
KW DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1047
FT /note="Error-prone DNA polymerase"
FT /id="PRO_0000103382"
SQ SEQUENCE 1047 AA; 116728 MW; 6CA4ACD601B82A94 CRC64;
MACSAEAVFA ELHALSNFSF LRGASHPKEL VAEAARLGYA ALALTDECSL AGVVRAHAEA
KTLGFKLIIG SEFRLEDGPK LVLLAMDRRG YGQLAAFITL GRRAAEKGEY RLLRGQLETH
PLDQCIAIWL PEDAPDDETG AWLARLFPAR CWIGVELFLS GGDGLRLSVL QALGERLGLP
LVACNDVHMH ARERQPLQDT LTAIRLGRPL AELGYALFPN GERHLRPIEF LARIYPRALL
EESLRIAERC RFSLDELRYE YPAELVPDGY TAIAWLRELT RQGMAERWPE GAPDKVRRQV
EHELELIGAM AYEPFFLTVH DVVRFARSRG ILCQGRGSAA NSAVCYCLGI TEVDPARLDL
LFERFISRER NEPPDIDVDF EHERREEVIQ YIYRKYGRHR AALAASLITY RVRSAVRDVA
RALGFSPSRI DTLARVLDRH GVAETLPERL AEAGLASENP AVRRLLALMQ MLVGFPRHLS
QHVGGFVIAA EDLSHWVPVE NAAMPERTVI QWDKDDLESL GLLKVDVLSL GMLTAIRKAL
AYVGEGRGRP FTLADVPPED PAVYEMLQRA DSIGVFQVES RAQMSMLPRL RPQSYYDLVI
QIAIVRPGPI QGDMVHPYLT RRAGLEPVSY PSPAVEKVLK RTLGVPIFQE QVMQLAMVAA
GFTPGEADRL RRAMAAWHRK GGLEPFEKKL MDGMRERGYE ERFARQIYRQ IRGFGEYGFP
ESHSASFALL AYVSAWLKCH HPAAFACALL NSQPMGFYGP SQLIQDARRH GVEVRPIDVN
HSDWDCTLEP TEIPNPLSLW ERVGVRALQT GSNPALRLGL RLVNGFSSAA AQRLSDARRQ
GPFQSIQDLA TRVPLDRREL EALAAADALH GLGGHRHRAF WEAAGVEAPT PLYAEPQFAE
AEPLLRKPGE AEDVIADYAA AGASLRRHPL SLLRERLDRR GFRTAEALWQ VRNGAIARVA
GLVVCRQRPM TANGTTFVTI EDETGQINLI VWPATAQAQR RALLRAHLLA VSGTVQQEEG
VLHLVAGRLE DIGKWLDGLV VKSRDFQ
//
