ID DPOG_YEAST Reviewed; 1254 AA.
AC P15801; D6W327; Q08785;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 3.
DT 27-MAR-2024, entry version 177.
DE RecName: Full=DNA polymerase gamma;
DE EC=2.7.7.7;
DE AltName: Full=Mitochondrial DNA polymerase catalytic subunit;
GN Name=MIP1; OrderedLocusNames=YOR330C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=2684980; DOI=10.1016/s0021-9258(19)47098-1;
RA Foury F.;
RT "Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic
RT subunit of the yeast mitochondrial DNA polymerase.";
RL J. Biol. Chem. 264:20552-20560(1989).
RN [2]
RP SEQUENCE REVISION.
RA Foury F.;
RL Submitted (JUN-1990) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8896263;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<999::aid-yea976>3.0.co;2-e;
RA Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.;
RT "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces
RT cerevisiae chromosome XV: similarity to part of chromosome I.";
RL Yeast 12:999-1004(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE OF 987-1254.
RC STRAIN=S288c / GRF88;
RA Song J.M., Cheung E., Rabinowitz J.C.;
RL Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in the replication of mitochondrial DNA.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha,
CC beta, gamma, delta, and epsilon which are responsible for different
CC reactions of DNA synthesis.
CC -!- MISCELLANEOUS: Present with 377 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA99652.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J05117; AAA17543.1; -; Unassigned_DNA.
DR EMBL; Z49821; CAA89977.1; -; Genomic_DNA.
DR EMBL; Z75238; CAA99652.1; ALT_INIT; Genomic_DNA.
DR EMBL; U42227; AAA85442.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11093.1; -; Genomic_DNA.
DR PIR; S62062; A32686.
DR RefSeq; NP_014975.2; NM_001183750.1.
DR AlphaFoldDB; P15801; -.
DR SMR; P15801; -.
DR BioGRID; 34715; 164.
DR IntAct; P15801; 3.
DR STRING; 4932.YOR330C; -.
DR GlyGen; P15801; 2 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; P15801; -.
DR MaxQB; P15801; -.
DR PaxDb; 4932-YOR330C; -.
DR PeptideAtlas; P15801; -.
DR EnsemblFungi; YOR330C_mRNA; YOR330C; YOR330C.
DR GeneID; 854508; -.
DR KEGG; sce:YOR330C; -.
DR AGR; SGD:S000005857; -.
DR SGD; S000005857; MIP1.
DR VEuPathDB; FungiDB:YOR330C; -.
DR eggNOG; KOG3657; Eukaryota.
DR GeneTree; ENSGT00390000000453; -.
DR HOGENOM; CLU_001524_2_1_1; -.
DR InParanoid; P15801; -.
DR OMA; LWLWDED; -.
DR OrthoDB; 5403095at2759; -.
DR BioCyc; YEAST:G3O-33807-MONOMER; -.
DR BioGRID-ORCS; 854508; 8 hits in 10 CRISPR screens.
DR PHI-base; PHI:505; -.
DR PRO; PR:P15801; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; P15801; Protein.
DR GO; GO:0005760; C:gamma DNA polymerase complex; IEA:InterPro.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IDA:SGD.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IDA:SGD.
DR GO; GO:0032043; P:mitochondrial DNA catabolic process; IMP:SGD.
DR GO; GO:0006264; P:mitochondrial DNA replication; IDA:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR CDD; cd08641; DNA_pol_gammaA; 1.
DR Gene3D; 3.30.420.390; -; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR002297; DNA-dir_DNA_pol_A_mt.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR041336; DNApol_Exo.
DR InterPro; IPR047580; POLG_palm_dom.
DR InterPro; IPR012337; RNaseH-like_sf.
DR PANTHER; PTHR10267; DNA POLYMERASE SUBUNIT GAMMA-1; 1.
DR PANTHER; PTHR10267:SF0; DNA POLYMERASE SUBUNIT GAMMA-1; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF18136; DNApol_Exo; 1.
DR PIRSF; PIRSF000797; DNA_pol_mt; 1.
DR PRINTS; PR00867; DNAPOLG.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 1: Evidence at protein level;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium;
KW Mitochondrion; Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1..1254
FT /note="DNA polymerase gamma"
FT /id="PRO_0000101278"
FT REGION 1125..1145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1202..1240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1206..1220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 8
FT /note="S -> F (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 35
FT /note="T -> A (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="I -> V (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="E -> K (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 540..541
FT /note="TH -> MN (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 616
FT /note="S -> N (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 661
FT /note="A -> T (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 978
FT /note="S -> P (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
FT CONFLICT 986
FT /note="N -> S (in Ref. 1; AAA17543)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1254 AA; 143502 MW; 80EE686E8EA9F2EC CRC64;
MTKLMVRSEC MLRMVRRRPL RVQFCARWFS TKKNTAEAPR INPVGIQYLG ESLQRQVFGS
CGGKDEVEQS DKLMELSKKS LKDHGLWGKK TLITDPISFP LPPLQGRSLD EHFQKIGRFN
SEPYKSFCED KFTEMVARPA EWLRKPGWVK YVPGMAPVEV AYPDEELVVF DVETLYNVSD
YPTLATALSS TAWYLWCSPF ICGGDDPAAL IPLNTLNKEQ VIIGHNVAYD RARVLEEYNF
RDSKAFFLDT QSLHIASFGL CSRQRPMFMK NNKKKEAEVE SEVHPEISIE DYDDPWLNVS
ALNSLKDVAK FHCKIDLDKT DRDFFASTDK STIIENFQKL VNYCATDVTA TSQVFDEIFP
VFLKKCPHPV SFAGLKSLSK CILPTKLNDW NDYLNSSESL YQQSKVQIES KIVQIIKDIV
LLKDKPDFYL KDPWLSQLDW TTKPLRLTKK GVPAKCQKLP GFPEWYRQLF PSKDTVEPKI
TIKSRIIPIL FKLSWENSPV IWSKESGWCF NVPHEQVETY KAKNYVLADS VSQEEEEIRT
HNLGLQCTGV LFKVPHPNGP TFNCTNLLTK SYNHFFEKGV LKSESELAHQ ALQINSSGSY
WMSARERIQS QFVVPSCKFP NEFQSLSAKS SLNNEKTNDL AIIIPKIVPM GTITRRAVEN
AWLTASNAKA NRIGSELKTQ VKAPPGYCFV GADVDSEELW IASLVGDSIF NVHGGTAIGW
MCLEGTKNEG TDLHTKTAQI LGCSRNEAKI FNYGRIYGAG AKFASQLLKR FNPSLTDEET
KKIANKLYEN TKGKTKRSKL FKKFWYGGSE SILFNKLESI AEQETPKTPV LGCGITYSLM
KKNLRANSFL PSRINWAIQS SGVDYLHLLC CSMEYIIKKY NLEARLCISI HDEIRFLVSE
KDKYRAAMAL QISNIWTRAM FCQQMGINEL PQNCAFFSQV DIDSVIRKEV NMDCITPSNK
TAIPHGEALD INQLLDKSNS KLGKPNLDID SKVSQYAYNY REPVFEEYNK SYTPEFLKYF
LAMQVQSDKR DVNRLEDEYL RECTSKEYAR DGNTAEYSLL DYIKDVEKGK RTKVRIMGSN
FLDGTKNAKA DQRIRLPVNM PDYPTLHKIA NDSAIPEKQL LENRRKKENR IDDENKKKLT
RKKNTTPMER KYKRVYGGRK AFEAFYECAN KPLDYTLETE KQFFNIPIDG VIDDVLNDKS
NYKKKPSQAR TASSSPIRKT AKAVHSKKLP ARKSSTTNRN LVELERDITI SREY
//
