ID DXR_PLAF7 Reviewed; 488 AA.
AC Q8IKG4; A0A144A2T4;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 27-MAR-2024, entry version 120.
DE RecName: Full=1-deoxy-D-xylulose 5-phosphate reductoisomerase, apicoplastic;
DE Short=1-deoxyxylulose-5-phosphate reductoisomerase;
DE Short=DOXP reductoisomerase;
DE Short=DXP reductoisomerase;
DE EC=1.1.1.267 {ECO:0000269|PubMed:25781377, ECO:0000269|PubMed:27487410};
DE Flags: Precursor;
GN Name=DXR; ORFNames=PF14_0641, PF3D7_1467300;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2] {ECO:0007744|PDB:4Y67, ECO:0007744|PDB:4Y6P, ECO:0007744|PDB:4Y6R, ECO:0007744|PDB:4Y6S}
RP X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 75-488 IN COMPLEX WITH MANGANESE
RP AND INHIBITORS, CATALYTIC ACTIVITY, COFACTOR, ACTIVITY REGULATION, AND
RP PATHWAY.
RX PubMed=25781377; DOI=10.1021/jm5014264;
RA Chofor R., Sooriyaarachchi S., Risseeuw M.D., Bergfors T., Pouyez J.,
RA Johny C., Haymond A., Everaert A., Dowd C.S., Maes L., Coenye T., Alex A.,
RA Couch R.D., Jones T.A., Wouters J., Mowbray S.L., Van Calenbergh S.;
RT "Synthesis and bioactivity of beta-substituted fosmidomycin analogues
RT targeting 1-deoxy-D-xylulose-5-phosphate reductoisomerase.";
RL J. Med. Chem. 58:2988-3001(2015).
RN [3] {ECO:0007744|PDB:5JAZ, ECO:0007744|PDB:5JBI, ECO:0007744|PDB:5JC1, ECO:0007744|PDB:5JMP, ECO:0007744|PDB:5JMW, ECO:0007744|PDB:5JNL, ECO:0007744|PDB:5JO0}
RP X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 75-488 IN COMPLEX WITH MANGANESE,
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ACTIVITY REGULATION, PATHWAY, AND
RP SUBUNIT.
RX PubMed=27487410; DOI=10.1002/cmdc.201600249;
RA Sooriyaarachchi S., Chofor R., Risseeuw M.D., Bergfors T., Pouyez J.,
RA Dowd C.S., Maes L., Wouters J., Jones T.A., Van Calenbergh S.,
RA Mowbray S.L.;
RT "Targeting an Aromatic Hotspot in Plasmodium falciparum 1-Deoxy-d-xylulose-
RT 5-phosphate Reductoisomerase with beta-Arylpropyl Analogues of
RT Fosmidomycin.";
RL ChemMedChem 11:2024-2036(2016).
CC -!- FUNCTION: Catalyzes the NADPH-dependent rearrangement and reduction of
CC 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-
CC phosphate (MEP). {ECO:0000269|PubMed:25781377,
CC ECO:0000269|PubMed:27487410}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-
CC xylulose 5-phosphate + H(+) + NADPH; Xref=Rhea:RHEA:13717,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:57792,
CC ChEBI:CHEBI:58262, ChEBI:CHEBI:58349; EC=1.1.1.267;
CC Evidence={ECO:0000269|PubMed:25781377, ECO:0000269|PubMed:27487410};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:13719;
CC Evidence={ECO:0000305|PubMed:25781377, ECO:0000305|PubMed:27487410};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:O96693};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:25781377, ECO:0000269|PubMed:27487410};
CC Note=Binds 1 divalent cation per subunit. Mg(2+) or Mn(2+).
CC {ECO:0000269|PubMed:25781377, ECO:0000269|PubMed:27487410};
CC -!- ACTIVITY REGULATION: Inhibited by fosmidomycin and its derivatives.
CC {ECO:0000269|PubMed:25781377, ECO:0000269|PubMed:27487410}.
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis
CC via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-
CC phosphate: step 1/6. {ECO:0000305|PubMed:25781377,
CC ECO:0000305|PubMed:27487410}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:25781377,
CC ECO:0000305|PubMed:27487410}.
CC -!- SUBCELLULAR LOCATION: Plastid, apicoplast
CC {ECO:0000250|UniProtKB:O96693}.
CC -!- SIMILARITY: Belongs to the DXR family. {ECO:0000305}.
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DR EMBL; LN999946; CZU00370.1; -; Genomic_DNA.
DR RefSeq; XP_001348815.1; XM_001348779.1.
DR PDB; 4Y67; X-ray; 1.60 A; A/B=75-488.
DR PDB; 4Y6P; X-ray; 1.90 A; A/B=75-488.
DR PDB; 4Y6R; X-ray; 1.90 A; A/B=75-488.
DR PDB; 4Y6S; X-ray; 2.10 A; A/B=75-488.
DR PDB; 5JAZ; X-ray; 1.40 A; A/B=75-488.
DR PDB; 5JBI; X-ray; 1.70 A; A/B=75-488.
DR PDB; 5JC1; X-ray; 1.65 A; A/B=75-488.
DR PDB; 5JMP; X-ray; 1.70 A; A/B=75-488.
DR PDB; 5JMW; X-ray; 1.55 A; A/B=75-488.
DR PDB; 5JNL; X-ray; 1.60 A; A/B=75-488.
DR PDB; 5JO0; X-ray; 1.80 A; A/B=75-488.
DR PDBsum; 4Y67; -.
DR PDBsum; 4Y6P; -.
DR PDBsum; 4Y6R; -.
DR PDBsum; 4Y6S; -.
DR PDBsum; 5JAZ; -.
DR PDBsum; 5JBI; -.
DR PDBsum; 5JC1; -.
DR PDBsum; 5JMP; -.
DR PDBsum; 5JMW; -.
DR PDBsum; 5JNL; -.
DR PDBsum; 5JO0; -.
DR AlphaFoldDB; Q8IKG4; -.
DR SMR; Q8IKG4; -.
DR STRING; 36329.Q8IKG4; -.
DR BindingDB; Q8IKG4; -.
DR ChEMBL; CHEMBL4295619; -.
DR PaxDb; 5833-PF14_0641; -.
DR EnsemblProtists; CZU00370; CZU00370; PF3D7_1467300.
DR GeneID; 812223; -.
DR KEGG; pfa:PF3D7_1467300; -.
DR VEuPathDB; PlasmoDB:PF3D7_1467300; -.
DR HOGENOM; CLU_035714_4_0_1; -.
DR InParanoid; Q8IKG4; -.
DR OMA; MMNKGFE; -.
DR OrthoDB; 275538at2759; -.
DR PhylomeDB; Q8IKG4; -.
DR BRENDA; 1.1.1.267; 4889.
DR UniPathway; UPA00056; UER00092.
DR Proteomes; UP000001450; Chromosome 14.
DR GO; GO:0020011; C:apicoplast; IDA:GeneDB.
DR GO; GO:0030604; F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0070402; F:NADPH binding; IEA:InterPro.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.1740.10; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR HAMAP; MF_00183; DXP_reductoisom; 1.
DR InterPro; IPR003821; DXP_reductoisomerase.
DR InterPro; IPR013644; DXP_reductoisomerase_C.
DR InterPro; IPR013512; DXP_reductoisomerase_N.
DR InterPro; IPR026877; DXPR_C.
DR InterPro; IPR036169; DXPR_C_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR00243; Dxr; 1.
DR PANTHER; PTHR30525; 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE; 1.
DR PANTHER; PTHR30525:SF0; 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE, CHLOROPLASTIC; 1.
DR Pfam; PF08436; DXP_redisom_C; 1.
DR Pfam; PF02670; DXP_reductoisom; 1.
DR Pfam; PF13288; DXPR_C; 1.
DR SUPFAM; SSF69055; 1-deoxy-D-xylulose-5-phosphate reductoisomerase, C-terminal domain; 1.
DR SUPFAM; SSF55347; Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Apicoplast; Isoprene biosynthesis; Magnesium; Manganese;
KW Metal-binding; NADP; Oxidoreductase; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..72
FT /note="Apicoplast"
FT /evidence="ECO:0000305"
FT CHAIN 73..488
FT /note="1-deoxy-D-xylulose 5-phosphate reductoisomerase,
FT apicoplastic"
FT /id="PRO_0000415609"
FT BINDING 86..89
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:O96693"
FT BINDING 115..117
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:O96693"
FT BINDING 205
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 206
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:O96693"
FT BINDING 231
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000269|PubMed:25781377,
FT ECO:0000269|PubMed:27487410, ECO:0007744|PDB:4Y67,
FT ECO:0007744|PDB:4Y6P, ECO:0007744|PDB:4Y6R,
FT ECO:0007744|PDB:4Y6S, ECO:0007744|PDB:5JAZ,
FT ECO:0007744|PDB:5JBI, ECO:0007744|PDB:5JC1,
FT ECO:0007744|PDB:5JMP, ECO:0007744|PDB:5JMW,
FT ECO:0007744|PDB:5JNL, ECO:0007744|PDB:5JO0"
FT BINDING 232
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 233
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 233
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000269|PubMed:25781377,
FT ECO:0000269|PubMed:27487410, ECO:0007744|PDB:4Y67,
FT ECO:0007744|PDB:4Y6P, ECO:0007744|PDB:4Y6R,
FT ECO:0007744|PDB:4Y6S, ECO:0007744|PDB:5JAZ,
FT ECO:0007744|PDB:5JBI, ECO:0007744|PDB:5JC1,
FT ECO:0007744|PDB:5JMP, ECO:0007744|PDB:5JMW,
FT ECO:0007744|PDB:5JNL, ECO:0007744|PDB:5JO0"
FT BINDING 270
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 293
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 299
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:O96693"
FT BINDING 306
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 311
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 312
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 315
FT /ligand="1-deoxy-D-xylulose 5-phosphate"
FT /ligand_id="ChEBI:CHEBI:57792"
FT /evidence="ECO:0000250|UniProtKB:P45568"
FT BINDING 315
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000269|PubMed:25781377,
FT ECO:0000269|PubMed:27487410, ECO:0007744|PDB:4Y67,
FT ECO:0007744|PDB:4Y6P, ECO:0007744|PDB:4Y6R,
FT ECO:0007744|PDB:4Y6S, ECO:0007744|PDB:5JAZ,
FT ECO:0007744|PDB:5JBI, ECO:0007744|PDB:5JC1,
FT ECO:0007744|PDB:5JMP, ECO:0007744|PDB:5JMW,
FT ECO:0007744|PDB:5JNL, ECO:0007744|PDB:5JO0"
FT STRAND 78..84
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 88..103
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 107..116
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 118..128
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 131..136
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 141..148
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 151..153
FT /evidence="ECO:0007829|PDB:5JNL"
FT STRAND 157..160
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 161..170
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 176..179
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 184..195
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 205..221
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 226..229
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 232..239
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 243..246
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 251..253
FT /evidence="ECO:0007829|PDB:5JMW"
FT HELIX 256..259
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 262..269
FT /evidence="ECO:0007829|PDB:5JAZ"
FT TURN 273..276
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 279..284
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 287..291
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 300..308
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 310..323
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 327..329
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 330..334
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 340..346
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 351..355
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 361..369
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 383..386
FT /evidence="ECO:0007829|PDB:5JAZ"
FT STRAND 388..390
FT /evidence="ECO:0007829|PDB:5JAZ"
FT TURN 396..398
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 400..411
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 415..431
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 437..450
FT /evidence="ECO:0007829|PDB:5JAZ"
FT HELIX 460..485
FT /evidence="ECO:0007829|PDB:5JAZ"
SQ SEQUENCE 488 AA; 55757 MW; 4E280C81CDFAD3EF CRC64;
MKKYIYIYFF FITITINDLV INNTSKCVSI ERRKNNAYIN YGIGYNGPDN KITKSRRCKR
IKLCKKDLID IGAIKKPINV AIFGSTGSIG TNALNIIREC NKIENVFNVK ALYVNKSVNE
LYEQAREFLP EYLCIHDKSV YEELKELVKN IKDYKPIILC GDEGMKEICS SNSIDKIVIG
IDSFQGLYST MYAIMNNKIV ALANKESIVS AGFFLKKLLN IHKNAKIIPV DSEHSAIFQC
LDNNKVLKTK CLQDNFSKIN NINKIFLCSS GGPFQNLTMD ELKNVTSENA LKHPKWKMGK
KITIDSATMM NKGLEVIETH FLFDVDYNDI EVIVHKECII HSCVEFIDKS VISQMYYPDM
QIPILYSLTW PDRIKTNLKP LDLAQVSTLT FHKPSLEHFP CIKLAYQAGI KGNFYPTVLN
ASNEIANNLF LNNKIKYFDI SSIISQVLES FNSQKVSENS EDLMKQILQI HSWAKDKATD
IYNKHNSS
//
