ID E0NNM4_9FIRM Unreviewed; 528 AA.
AC E0NNM4;
DT 02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=hsdM {ECO:0000313|EMBL:EFM24627.1};
GN ORFNames=HMPREF9225_1763 {ECO:0000313|EMBL:EFM24627.1};
OS Peptoniphilus duerdenii ATCC BAA-1640.
OC Bacteria; Bacillota; Tissierellia; Tissierellales; Peptoniphilaceae;
OC Peptoniphilus.
OX NCBI_TaxID=862517 {ECO:0000313|EMBL:EFM24627.1, ECO:0000313|Proteomes:UP000003280};
RN [1] {ECO:0000313|EMBL:EFM24627.1, ECO:0000313|Proteomes:UP000003280}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1640 {ECO:0000313|EMBL:EFM24627.1,
RC ECO:0000313|Proteomes:UP000003280};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFM24627.1}.
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DR EMBL; AEEH01000050; EFM24627.1; -; Genomic_DNA.
DR AlphaFoldDB; E0NNM4; -.
DR STRING; 862517.HMPREF9225_1763; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_013049_0_1_9; -.
DR Proteomes; UP000003280; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EFM24627.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000003280};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EFM24627.1}.
FT DOMAIN 19..171
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 185..493
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 497..528
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 528 AA; 60226 MW; E1D441172A3B831A CRC64;
MKMSNLENQN NESLQRAELH RKIWAIADNV RGAVDGWDFK QYILGILFYR FISENITEFF
NSAEHEAGDL DFDYADLSDE EAERDFRPGT VEDKGFFILP SELFVNVCKD ARTNENLNTD
LANIFKAIEG SAVGYPSEDD IKGLFEDVDT KSNRLGGNVP EKNKRLADIL TGIAEINFGE
FQKNDIDAFG DAYEYLISNY ASNAGKSGGE FFTPQTVSKL LARIVMDGKT SINKAYDPTC
GSGSLLLQMK KQFEEHIIDE GFFGQEINMT NFNLARMNMF LHNVNYNNFS IKRGDTLLQP
KHKDEKPFDA IVSNPPYSIK WIGDADPTLI NDERFAPAGK LAPKSYADYA FILHSLSYLS
SKGRAAIVCF PGIFYRKGAE KTIRQYLVDN NFIDCVIQLP ENLFFGTSIA TCILVMAKNK
TENKILFIDA SKEFKKETNN NILEQKNIDA IVEEFRNRDE KEYFSRYVPV EEIVENDYNL
SVSTYVEKED TREKIDIKVL NKEIEETVKK IDELRASINE IVKELEDE
//
