ID E0STH7_IGNAA Unreviewed; 805 AA.
AC E0STH7;
DT 02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN OrderedLocusNames=Igag_1971 {ECO:0000313|EMBL:ADM28763.1};
OS Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1).
OC Archaea; Thermoproteota; Thermoprotei; Desulfurococcales;
OC Desulfurococcaceae; Ignisphaera.
OX NCBI_TaxID=583356 {ECO:0000313|EMBL:ADM28763.1, ECO:0000313|Proteomes:UP000001304};
RN [1] {ECO:0000313|EMBL:ADM28763.1, ECO:0000313|Proteomes:UP000001304}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17230 / JCM 13409 / AQ1.S1
RC {ECO:0000313|Proteomes:UP000001304};
RX PubMed=21304693; DOI=10.4056/sigs.1072907;
RA Goker M., Held B., Lapidus A., Nolan M., Spring S., Yasawong M., Lucas S.,
RA Glavina Del Rio T., Tice H., Cheng J.F., Goodwin L., Tapia R., Pitluck S.,
RA Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA Palaniappan K., Brambilla E., Land M., Hauser L., Chang Y.J.,
RA Jeffries C.D., Brettin T., Detter J.C., Han C., Rohde M., Sikorski J.,
RA Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Ignisphaera aggregans type strain (AQ1.S1).";
RL Stand. Genomic Sci. 3:66-75(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755}.
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DR EMBL; CP002098; ADM28763.1; -; Genomic_DNA.
DR AlphaFoldDB; E0STH7; -.
DR STRING; 583356.Igag_1971; -.
DR KEGG; iag:Igag_1971; -.
DR HOGENOM; CLU_349731_0_0_2; -.
DR BioCyc; IAGG583356:GHAH-1960-MONOMER; -.
DR Proteomes; UP000001304; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000001304};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 403..667
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
SQ SEQUENCE 805 AA; 92315 MW; DBE26F5C8BC0B89E CRC64;
MLDVIIVWGE KGKVEKVIRV NEDGSFEEAQ HRPWFVALRF MGAKGIVDYR EVPLKFYVFS
EKTWRYEKAS DNSYIIYTET PSDVRRVATL YDDNGIPHAL SNIRYKARVS LDLSDSLFGL
KVPAILHYSL DELENVIREL IEKMKTVKVL GFDIEVEAKG SFPRPGDRVF IVSLCWDGLL
DDSEECVVLE GDSVFEFMKH IEKVRPNYIL GFVSNTFDIP YLTAYIKELQ LTREGILTDT
VAIPHIDLAE VLEQHGSGFG LPFGSRLALD DIAKKMALIK NVEELEIESS IDRNRIWLEY
QNVREKVVRY AETDAKLTLR IGKEVLKTLL SLYLLTGISP STIQVLPSFG SIAEYSVLDV
VRRKYGKVYQ VRSAKYTAKE LIDGIAFYKK GTKEHFLREG LWENVAYLDF NMLYPTIYYS
YKLDPEGVCI GNGFRIYLVD RDKDGDIAVK ESIPIDVSFT GGDVYDVLRY FYEGRKVTKK
IKKEFPGIDQ AMKILANSAY GMFSKGRGGA VNEVLAGFIF FKSSHIFQST LGIIKNMGLN
YVYGATDSFF VLLNNIDPKE LEEKLNRAIQ SRFGSEFSIK FEGICRKFVL LKRKTYVCVL
ENDEGKEVLI KGMEKLELPQ VVKDNLPEIF ERVLNGEDWR KVIDEYMKSA GIRELFVKVS
KRISDLYDEE ERRFKNPNSN RTKAILVKYL YDEGQHLSGS GHLQFYFTDD SLPNTSVVAY
YLNSNRSIAG KKAVMVLIDY DEGNATTYEC GLVDYKSRRE LSGTFFCVER RLSRSEVEEL
AKIKAGRVVD YLKQIETMTK QKKLL
//
