ID E0VLT0_PEDHC Unreviewed; 1659 AA.
AC E0VLT0;
DT 02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2010, sequence version 1.
DT 24-JAN-2024, entry version 65.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN Name=8229707 {ECO:0000313|EnsemblMetazoa:PHUM293360-PA};
GN ORFNames=Phum_PHUM293360 {ECO:0000313|EMBL:EEB14336.1};
OS Pediculus humanus subsp. corporis (Body louse).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Psocodea; Phthiraptera; Anoplura; Pediculidae;
OC Pediculus.
OX NCBI_TaxID=121224;
RN [1] {ECO:0000313|EMBL:EEB14336.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=USDA {ECO:0000313|EMBL:EEB14336.1};
RA Kirkness E., Hannick L., Hass B., Bruggner R., Lawson D., Bidwell S.,
RA Joardar V., Caler E., Walenz B., Inman J., Schobel S., Galinsky K.,
RA Amedeo P., Strausberg R.;
RT "Annotation of Pediculus humanus corporis strain USDA.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEB14336.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=USDA {ECO:0000313|EMBL:EEB14336.1};
RG The Human Body Louse Genome Consortium;
RA Kirkness E., Walenz B., Hass B., Bruggner R., Strausberg R.;
RT "The genome of the human body louse.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblMetazoa:PHUM293360-PA}
RP IDENTIFICATION.
RC STRAIN=USDA {ECO:0000313|EnsemblMetazoa:PHUM293360-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; AAZO01003399; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DS235281; EEB14336.1; -; Genomic_DNA.
DR RefSeq; XP_002427074.1; XM_002427029.1.
DR STRING; 121224.E0VLT0; -.
DR EnsemblMetazoa; PHUM293360-RA; PHUM293360-PA; PHUM293360.
DR GeneID; 8229707; -.
DR KEGG; phu:Phum_PHUM293360; -.
DR CTD; 8229707; -.
DR VEuPathDB; VectorBase:PHUM293360; -.
DR eggNOG; KOG0262; Eukaryota.
DR HOGENOM; CLU_000487_2_4_1; -.
DR InParanoid; E0VLT0; -.
DR OMA; NREDYQQ; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000009046; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0031981; C:nuclear lumen; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000009046};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 288..599
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1321..1429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1365..1381
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1382..1406
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1407..1429
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1659 AA; 189264 MW; B090E9B9D2C3E4DA CRC64;
MAVEKVEINK IIFGIFNASD IYNLSVVEIK TSICFNALDH PLKDGLYDLV MGPSGMLKES
CLTCRQTIRN CCGHFGHINL PLPVINPLFH KEVLTLLKLS CSYCYKLAIP PNLRNLFIYQ
MKLLDHGKMA EAEDLETKAL NLKEMDSNDA QNYVVKENYS EYLKELELDS NPVNIPSSRS
IESLRLKFLN EFIKQIILPK SCTHCKNPFK RIIKFQNKII SSITKVEIAK KFKDVPQNAF
NKGSKQETTF ITPKESRETL RKIWEESKDL VEELMPILKN SNLSYPTDLF FMDVILVTPP
KARPVEFANV KAVDTGDYNS FPPEYKVYID GIRGATVLEK LHECWLQLQN DIDTLLDSSA
ARISLKKGIS GLKQVLEKKE GVLRMHMMGK RVNFAARTVI TPDPNLNIQE IGVPEYFAKK
LTYPVGVNFF NVEELRKMVQ NGPDVYPGAT LIENENGSLI KLTGGEENRK KRIGISKLLL
KSIEGDPTSF KTKKVHRHLL NGDIVLLNRQ PTLHRPSIMA HIVKILKNEK TFRLHYSNCK
AYNADFDGDE MNLHLPQTEL SRSEGYNIVN VANHYLVPKD GTPLGGLIQD HIVSTVRLTM
KNQMFEKEDY HQLVYQGLSH KNGNIILLPP CIVKPKKLWS GKQIISTILI NIIPKDKDLI
SFNGNAKISV KLWETGSPRK WMCGEEFKSD NEMSESSIIL RKGILVTGVL DKQHFGATPF
GLTHCLYELY GGECSSKFLN SIGRLSTAFL QMTGFTLGVE DILVKAKADK SRKKIIEKLR
KCGDEIAAKA LGLEEIPNDL YERLEREYFK NRTMFKGKLD QEYKVKLNSF TNEINKVCLP
KGLISRFPEN NLQLMVTSGA KGSTVNTMQI SCLLGQIELE GKRPPFMISG KSLPSYKAFD
TQPCAGGYID NRFMTGISPQ EFFFHCMAGR EGLIDTAVKT SRSGYLQRCL IKHLEGVSIG
YDGTVRDHDG TVIQFIYGGD GLEVQKSTFF KPTHMEFLHN NLNAVVKKSV IESLKADVPY
KKIEAYREEV MAGINLSKKV LAPFTSFSQS IKNDIRNKYF TKLDKRTGRT KAYSKMLKKW
NALADEDKQL YKQEVRLDPV TSKYNRNHDF GVITEKLETL IKDYLDAKCS NQSKKEKKKV
KNMVEAKYML SLCQPGEPVG LLAAQSIGEP STQMTLNTFH FAGRGEMNVT LGIPRLREIL
MTASKSIKTP YVDVPLKKKS EKKMLKNGNK LKKIFQRVTL ADVLETITVH EKVIFKPSPL
FTYTIRFKFL PHSFYKDDYL IKPKDVLEYV EKPFLILLCK EIRKLSKKFG DIIFESKEEK
MISKEDEEKD DADNVDSAGV NFNKYRGFGE NHESSDEEEE LDDDDATKEK VRNRHCENQE
YDEPEESEEE KSDEENDTLY QDEPSEYEEE TERRVELGEE ESESRDIPEI NLTSEELKNV
ENRKNFVLNT FPMIKKYDFD VVKEEWCEMV VGIPLTLNKI DFSTILKVIC GKSVLYQIPN
VRRAFVYKNP SGELHLKTEG VNFNEMFKFY KMIDINRLYS NDVNKVVETL GIEAGVRIIV
KELNDVFGVY GITVDHRHLL LVADYMTNQG NYSPFNRIGL ADSSSPLQQM SFERSTGFLK
KSLVRNQEDF MNSPSARIMA GRFCKSGTGC FDVMTLPSI
//
