ID E1BJ31_BOVIN Unreviewed; 975 AA.
AC E1BJ31;
DT 02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=receptor protein-tyrosine kinase {ECO:0000256|ARBA:ARBA00011902};
DE EC=2.7.10.1 {ECO:0000256|ARBA:ARBA00011902};
GN Name=EPHA2 {ECO:0000313|Ensembl:ENSBTAP00000001080.4,
GN ECO:0000313|VGNC:VGNC:28534};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000001080.4, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000001080.4, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000001080.4,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000001080.4}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000001080.4};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001171};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004251};
CC Single-pass type I membrane protein {ECO:0000256|ARBA:ARBA00004251}.
CC Membrane {ECO:0000256|ARBA:ARBA00004479}; Single-pass type I membrane
CC protein {ECO:0000256|ARBA:ARBA00004479}.
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DR RefSeq; NP_001192660.1; NM_001205731.1.
DR AlphaFoldDB; E1BJ31; -.
DR SMR; E1BJ31; -.
DR STRING; 9913.ENSBTAP00000001080; -.
DR PaxDb; 9913-ENSBTAP00000001080; -.
DR Ensembl; ENSBTAT00000001080.5; ENSBTAP00000001080.4; ENSBTAG00000000815.5.
DR GeneID; 512798; -.
DR KEGG; bta:512798; -.
DR CTD; 1969; -.
DR VEuPathDB; HostDB:ENSBTAG00000000815; -.
DR VGNC; VGNC:28534; EPHA2.
DR eggNOG; KOG0196; Eukaryota.
DR GeneTree; ENSGT00940000160786; -.
DR HOGENOM; CLU_000288_141_0_1; -.
DR InParanoid; E1BJ31; -.
DR OMA; CLECPVH; -.
DR OrthoDB; 1614410at2759; -.
DR TreeFam; TF315608; -.
DR Reactome; R-BTA-2682334; EPH-Ephrin signaling.
DR Reactome; R-BTA-3928663; EPHA-mediated growth cone collapse.
DR Reactome; R-BTA-3928665; EPH-ephrin mediated repulsion of cells.
DR Reactome; R-BTA-9013149; RAC1 GTPase cycle.
DR Reactome; R-BTA-9013404; RAC2 GTPase cycle.
DR Reactome; R-BTA-9013408; RHOG GTPase cycle.
DR Reactome; R-BTA-9013420; RHOU GTPase cycle.
DR Reactome; R-BTA-9013423; RAC3 GTPase cycle.
DR Reactome; R-BTA-9013424; RHOV GTPase cycle.
DR Reactome; R-BTA-9696264; RND3 GTPase cycle.
DR Reactome; R-BTA-9696270; RND2 GTPase cycle.
DR Reactome; R-BTA-9696273; RND1 GTPase cycle.
DR Proteomes; UP000009136; Chromosome 2.
DR Bgee; ENSBTAG00000000815; Expressed in esophagus and 102 other cell types or tissues.
DR ExpressionAtlas; E1BJ31; baseline.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005003; F:ephrin receptor activity; IEA:InterPro.
DR GO; GO:0004714; F:transmembrane receptor protein tyrosine kinase activity; IBA:GO_Central.
DR GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; IBA:GO_Central.
DR CDD; cd10480; EphR_LBD_A2; 1.
DR CDD; cd00063; FN3; 2.
DR CDD; cd09543; SAM_EPH-A2; 1.
DR Gene3D; 2.60.40.1770; ephrin a2 ectodomain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR Gene3D; 1.20.5.510; Single helix bin; 1.
DR Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 2.10.50.10; Tumor Necrosis Factor Receptor, subunit A, domain 2; 1.
DR InterPro; IPR027936; Eph_TM.
DR InterPro; IPR034263; EphA2_rcpt_lig-bd.
DR InterPro; IPR001090; Ephrin_rcpt_lig-bd_dom.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR InterPro; IPR020635; Tyr_kinase_cat_dom.
DR InterPro; IPR016257; Tyr_kinase_ephrin_rcpt.
DR InterPro; IPR001426; Tyr_kinase_rcpt_V_CS.
DR PANTHER; PTHR46877; EPH RECEPTOR A5; 1.
DR PANTHER; PTHR46877:SF12; EPHRIN TYPE-A RECEPTOR 3; 1.
DR Pfam; PF14575; EphA2_TM; 1.
DR Pfam; PF01404; Ephrin_lbd; 1.
DR Pfam; PF00041; fn3; 2.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR Pfam; PF00536; SAM_1; 1.
DR PIRSF; PIRSF000666; TyrPK_ephrin_receptor; 1.
DR PRINTS; PR00109; TYRKINASE.
DR SMART; SM00615; EPH_lbd; 1.
DR SMART; SM01411; Ephrin_rec_like; 1.
DR SMART; SM00060; FN3; 2.
DR SMART; SM00454; SAM; 1.
DR SMART; SM00219; TyrKc; 1.
DR SUPFAM; SSF49265; Fibronectin type III; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF57184; Growth factor receptor domain; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR PROSITE; PS51550; EPH_LBD; 1.
DR PROSITE; PS50853; FN3; 2.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR PROSITE; PS00790; RECEPTOR_TYR_KIN_V_1; 1.
DR PROSITE; PS00791; RECEPTOR_TYR_KIN_V_2; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRSR:PIRSR000666-
KW 2}; Disulfide bond {ECO:0000256|PIRSR:PIRSR000666-3};
KW Kinase {ECO:0000256|ARBA:ARBA00023137};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRSR:PIRSR000666-2};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Receptor {ECO:0000256|ARBA:ARBA00023170};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00023137};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Tyrosine-protein kinase {ECO:0000256|ARBA:ARBA00023137}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..975
FT /note="receptor protein-tyrosine kinase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003144056"
FT TRANSMEM 535..558
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 28..206
FT /note="Eph LBD"
FT /evidence="ECO:0000259|PROSITE:PS51550"
FT DOMAIN 328..437
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000259|PROSITE:PS50853"
FT DOMAIN 438..529
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000259|PROSITE:PS50853"
FT DOMAIN 613..874
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT DOMAIN 903..967
FT /note="SAM"
FT /evidence="ECO:0000259|PROSITE:PS50105"
FT ACT_SITE 738
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000666-1"
FT BINDING 619..627
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR000666-2"
FT BINDING 645
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR000666-2,
FT ECO:0000256|PROSITE-ProRule:PRU10141"
FT DISULFID 70..188
FT /evidence="ECO:0000256|PIRSR:PIRSR000666-3"
FT DISULFID 105..115
FT /evidence="ECO:0000256|PIRSR:PIRSR000666-3"
SQ SEQUENCE 975 AA; 107918 MW; D09768D6C1CAF60D CRC64;
MGRRAPPACL ALLWGCALAA ASAAQGKEVV LLDFAAAKGE LGWLTHPYGK GWDLLQNIMN
DMPIYMYSVC NVVAGDQDNW LRTNWVYRGE AERIFIELKF TVRDCNSFPG GASSCKETFN
LYYAESDVDY GTNFQKRQFT KIDTIAPDEI TGSSDFEARH VKLNVEERSV GPLTRKGFYL
AFQDVGACVA LLSVRVYYKK CPELLKGLAR FPETIAGSDA PALATVAGNC VEHAVVPPGG
EEPRMHCAVD GEWLVPIGQC LCQAGYEKVE DACQACSPGF FKPEASESPC LECPAHTLPS
SEGATACECE DGYFRAPQDP LSLPCTRPPS APHYLTAVGM GAKVELRWTP PQDSGGREDL
TYSVSCEQCW PESGECGPCE ASVRYSEPPL GLTRTSVTVS DLEPHMNYTF TVEARNGVSG
LVSTRSFRTA SVSINQTEPP KVKLESRSTT SLSISWIIPP LQQSRVWKYE VTYRKKGDSN
SYNVRRTEGF SVTLDDLAPD TVYLVQVQAL TQEGQGAGSK VHEFQTLSTG GSGNMAVVGG
VAVGAVLLLV LAGIGFFIHR RRKSLRTRQS SEDVYFSKSE QLKPLKTYVD PHTYEDPNQA
VLKFTTEIHP SCVTRQKVIG AGEFGEVYKG TLKASGKKEV PVAIKTLKAG YTEKQRVDFL
SEASIMGQFS HHNIIRLEGV VSKYKPLMII TEYMENGALD KFLREKDGEF SVLQLVGMLR
GVAAGMKYLA NMSYVHRDLA ARNILVNSNL VCKVSDFGLS RVLEDDPEAT YTTSGGKIPI
RWTAPEAISY RKFTSASDVW SYGIVMWEVM TYGERPYWEL SNHEVMKAIN DGFRLPTPMD
CPSAIYQLMM QCWQQERTRR PKFADIVSIL DKLIRAPDSL KTLADFDPRV SIRLPSTSGS
EGVPFRTVSE WLESIKMQQY TEHFLVAGYT TIEKVVQMTN DDIKRIGVRL PGHQKRIAYS
LLGLKDQVNT VGVPI
//
