ID E1QDI8_DESB2 Unreviewed; 724 AA.
AC E1QDI8;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=Peptidase S45 penicillin amidase {ECO:0000313|EMBL:ADK83507.1};
GN OrderedLocusNames=Deba_0128 {ECO:0000313|EMBL:ADK83507.1};
OS Desulfarculus baarsii (strain ATCC 33931 / DSM 2075 / LMG 7858 / VKM B-1802
OS / 2st14).
OC Bacteria; Thermodesulfobacteriota; Desulfarculia; Desulfarculales;
OC Desulfarculaceae; Desulfarculus.
OX NCBI_TaxID=644282 {ECO:0000313|EMBL:ADK83507.1, ECO:0000313|Proteomes:UP000009047};
RN [1] {ECO:0000313|EMBL:ADK83507.1, ECO:0000313|Proteomes:UP000009047}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33931 / DSM 2075 / LMG 7858 / VKM B-1802 / 2st14
RC {ECO:0000313|Proteomes:UP000009047};
RX PubMed=21304732; DOI=10.4056/sigs.1243258;
RA Sun H., Spring S., Lapidus A., Davenport K., Del Rio T.G., Tice H.,
RA Nolan M., Copeland A., Cheng J.F., Lucas S., Tapia R., Goodwin L.,
RA Pitluck S., Ivanova N., Pagani I., Mavromatis K., Ovchinnikova G., Pati A.,
RA Chen A., Palaniappan K., Hauser L., Chang Y.J., Jeffries C.D., Detter J.C.,
RA Han C., Rohde M., Brambilla E., Goker M., Woyke T., Bristow J., Eisen J.A.,
RA Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P., Land M.;
RT "Complete genome sequence of Desulfarculus baarsii type strain (2st14).";
RL Stand. Genomic Sci. 3:276-284(2010).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
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DR EMBL; CP002085; ADK83507.1; -; Genomic_DNA.
DR RefSeq; WP_013256963.1; NC_014365.1.
DR AlphaFoldDB; E1QDI8; -.
DR STRING; 644282.Deba_0128; -.
DR MEROPS; S45.003; -.
DR KEGG; dbr:Deba_0128; -.
DR eggNOG; COG2366; Bacteria.
DR HOGENOM; CLU_011790_0_1_7; -.
DR OrthoDB; 9760084at2; -.
DR Proteomes; UP000009047; Chromosome.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000009047};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT ACT_SITE 220
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 293
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 296
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 724 AA; 80494 MW; 3499B423859CDCD6 CRC64;
MKLGHKKITL RGADGAVEII RGVGGVPVLE AASTRDMQFG LGWTHAADRQ LEALLMRVLL
RGRAAECLQG DPALIEIDKF MRLMDFLPQA DLEGEMAKLQ PQVRAELDSY CAGFNQGLAD
CGLIWEMRLM GYKPDPWSVA DCLLLGKAFG FVGLVDAQMG AQRLLIQMIQ NDLPEAKIRE
LFPYLSEPID YDLIKKIELS PPLVPEAVKW LAKLPKFQAS NNWAVSGRHT ASGAAFLCGD
PHLEVNRLPA IWQEIVAHLP DDTIIGACIP GAPGPIVGRT SRIAWSATYS FMDMLDFRIE
QCQGGKYRRG EQWREFRARQ EIIKVKKGQP ISFTVYENEH GVLEGDPFAE GHKLVLGWSA
RHGCGAGEFN GLLNLLRCQD VAQAMACFRE LDAATFNFVI ADDSGNIGYQ MSGRMFQRAP
GLSGLLPMPG WEAKHNHQGY VDKHKLPSQY NPAEGFIVTA NQDLNHLGTA KPINLPMAPY
RANRISQLLR QGRQMGVDYF KAMHFDLHSL QAERFMTLLR PLLPDSQQGR LLADWDLSYQ
ADSLGATAFE SVYLAMIRVV FGDGGMGRPV IDYVLTETGA FNDYYGNFDE VIMKADSAWF
DGRPREELLR RALEEGLAQP VKPYGQTRQI MLSHLLFGGK LPAWLGFDHG PIALPGGRAT
IPQGQIFKSA GRLTTFSPSL RFICDMATRQ LHSTLAGGPS DRRFGPWYVN GVQDWLAGNY
KLLE
//
