ID E1Z8R8_CHLVA Unreviewed; 526 AA.
AC E1Z8R8;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Dioxygenase {ECO:0008006|Google:ProtNLM};
GN ORFNames=CHLNCDRAFT_50902 {ECO:0000313|EMBL:EFN57655.1};
OS Chlorella variabilis (Green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX NCBI_TaxID=554065 {ECO:0000313|Proteomes:UP000008141};
RN [1] {ECO:0000313|EMBL:EFN57655.1, ECO:0000313|Proteomes:UP000008141}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NC64A {ECO:0000313|EMBL:EFN57655.1,
RC ECO:0000313|Proteomes:UP000008141};
RX PubMed=20852019; DOI=10.1105/tpc.110.076406;
RA Blanc G., Duncan G., Agarkova I., Borodovsky M., Gurnon J., Kuo A.,
RA Lindquist E., Lucas S., Pangilinan J., Polle J., Salamov A., Terry A.,
RA Yamada T., Dunigan D.D., Grigoriev I.V., Claverie J.M., Van Etten J.L.;
RT "The Chlorella variabilis NC64A genome reveals adaptation to
RT photosymbiosis, coevolution with viruses, and cryptic sex.";
RL Plant Cell 22:2943-2955(2010).
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
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DR EMBL; GL433839; EFN57655.1; -; Genomic_DNA.
DR RefSeq; XP_005849757.1; XM_005849695.1.
DR AlphaFoldDB; E1Z8R8; -.
DR STRING; 554065.E1Z8R8; -.
DR GeneID; 17357291; -.
DR KEGG; cvr:CHLNCDRAFT_50902; -.
DR eggNOG; KOG1285; Eukaryota.
DR InParanoid; E1Z8R8; -.
DR OMA; TEYMFAK; -.
DR OrthoDB; 34984at2759; -.
DR Proteomes; UP000008141; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR PANTHER; PTHR10543:SF37; CAROTENOID CLEAVAGE DIOXYGENASE 7, CHLOROPLASTIC; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00022964};
KW Reference proteome {ECO:0000313|Proteomes:UP000008141}.
FT BINDING 230
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 327
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 510
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 526 AA; 58349 MW; 54063732BF677313 CRC64;
MNTRVDDQYK SFLDNWDRGF VDTEEEPEGY WMEAEFGQIP MDLHGTLFRN GPGKFQIGSD
KVGHPYDGDG LVLAISFNNG SAFFRSRFVR TAEYLAESKE QRIMFRGTFG TQRPGGPAQN
ALDVVVKNTS NTNVVCWGGR LWTLFEAGQP YRLDPSSLET EGLETLDGQL RAGLPFDMGS
AGANEAFGSM ISGTQQRLGN GPYMPRELLA AEFEVAAKTS FELDGFAFLH DFAITKNFFV
VFQNPVTGRS WLLRPLLGSC FAKCRNAHPA VDNVPYVLGK APAAACVRWV AGKPTLLHLI
PRPGRTDAQG LPLQPRTFTA PSLFVFHHAN AYESEDGQRI VVDSIHYESL PAVGREALAE
QQIDPDVAFR SRLRRVEIDL RTGMLQIRKQ FDQYLEMCSI NEARSGRQHR FVFGYNSVFQ
PDNDVQIGIA KVDTVTGTAA LWRPGYQKFA LEPKFVGRPG ATKEDDGWLL TVMFDSGTQD
CHLVILDAQH LEAGPVASLK FRDPVPSGLH GCWSSTYYGP DAAPAQ
//
