ID E2ARG4_CAMFO Unreviewed; 543 AA.
AC E2ARG4;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093};
DE EC=3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093};
GN ORFNames=EAG_09586 {ECO:0000313|EMBL:EFN63973.1};
OS Camponotus floridanus (Florida carpenter ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Formicinae; Camponotus.
OX NCBI_TaxID=104421 {ECO:0000313|Proteomes:UP000000311};
RN [1] {ECO:0000313|EMBL:EFN63973.1, ECO:0000313|Proteomes:UP000000311}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C129 {ECO:0000313|Proteomes:UP000000311};
RX PubMed=20798317; DOI=10.1126/science.1192428;
RA Bonasio R., Zhang G., Ye C., Mutti N.S., Fang X., Qin N., Donahue G.,
RA Yang P., Li Q., Li C., Zhang P., Huang Z., Berger S.L., Reinberg D.,
RA Wang J., Liebig J.;
RT "Genomic comparison of the ants Camponotus floridanus and Harpegnathos
RT saltator.";
RL Science 329:1068-1071(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC Evidence={ECO:0000256|ARBA:ARBA00001231,
CC ECO:0000256|PIRNR:PIRNR001093};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family.
CC {ECO:0000256|ARBA:ARBA00006285, ECO:0000256|PIRNR:PIRNR001093}.
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DR EMBL; GL442063; EFN63973.1; -; Genomic_DNA.
DR RefSeq; XP_011262697.2; XM_011264395.2.
DR AlphaFoldDB; E2ARG4; -.
DR STRING; 104421.E2ARG4; -.
DR EnsemblMetazoa; XM_011264395.3; XP_011262697.2; LOC105255235.
DR GeneID; 105255235; -.
DR KEGG; cfo:105255235; -.
DR InParanoid; E2ARG4; -.
DR OMA; VVEVWPM; -.
DR OrthoDB; 178991at2759; -.
DR Proteomes; UP000000311; Unassembled WGS sequence.
DR GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd06562; GH20_HexA_HexB-like; 1.
DR Gene3D; 3.30.379.10; Chitobiase/beta-hexosaminidase domain 2-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR InterPro; IPR015883; Glyco_hydro_20_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR029018; Hex-like_dom2.
DR InterPro; IPR029019; HEX_eukaryotic_N.
DR PANTHER; PTHR22600; BETA-HEXOSAMINIDASE; 1.
DR PANTHER; PTHR22600:SF21; BETA-HEXOSAMINIDASE A; 1.
DR Pfam; PF00728; Glyco_hydro_20; 1.
DR Pfam; PF14845; Glycohydro_20b2; 1.
DR PIRSF; PIRSF001093; B-hxosamndse_ab_euk; 1.
DR PRINTS; PR00738; GLHYDRLASE20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF55545; beta-N-acetylhexosaminidase-like domain; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR001093-2};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR001093};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR001093};
KW Reference proteome {ECO:0000313|Proteomes:UP000000311};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..543
FT /note="Beta-hexosaminidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003157505"
FT DOMAIN 36..167
FT /note="Beta-hexosaminidase eukaryotic type N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14845"
FT DOMAIN 190..505
FT /note="Glycoside hydrolase family 20 catalytic"
FT /evidence="ECO:0000259|Pfam:PF00728"
FT ACT_SITE 345
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-1"
FT DISULFID 66..120
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-2"
FT DISULFID 298..350
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-2"
FT DISULFID 523..541
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-2"
SQ SEQUENCE 543 AA; 61853 MW; B9AA57C678D1DFC4 CRC64;
MWSRAIFLIL VTTAPWVACL WHADAGPWFE ATKGEVWPMP NSRVVKEDFY LLRPSNFDIR
VNGETCDIIT EAIERYMRII LTEARIARLV TEGQPRTSVR DDPHFKGTLE ALSIRLLKPC
EQNGDHWPHL YMNESYKLEI NETSSVAILR AEAVWGILRG LETFSQILAP SGGASLKVKC
QTIVDEPKLP HRGLLLDTSR HYLPLSDILL TLDAMSYNKL NVLHWHIVDD NSFPYQSTRY
PDLSAKGAYH QTMIYTPNDV QKVVDYARLR GIRVMPEFDT PGHTRSWGLA HPELLTICYD
SSRKPNGKLG PMNPTNPALY DFIRNLFSEI VQVFPDQYLH LGGDEVPFDC WGSNPEIGDY
MKAHNMSNRY ELLENQYIAK ILAISKSLDA NTIVWQEVFD NGVVLPTTTV VHVWKIPSWQ
KELERVTIAG HPVLLSSCWY LDHLSSGGDW EKYYNCNPFD FANAANATHL MLGGEACMWA
EFVNKNNVHS RIWPRASAAA ERLWSFNKQE NNVAAKRLEE HACRMNRRGI PAQPPNGPGF
CVT
//
