ID E2BRL5_HARSA Unreviewed; 1687 AA.
AC E2BRL5;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=EAI_14335 {ECO:0000313|EMBL:EFN81707.1};
OS Harpegnathos saltator (Jerdon's jumping ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Ponerinae; Ponerini; Harpegnathos.
OX NCBI_TaxID=610380 {ECO:0000313|Proteomes:UP000008237};
RN [1] {ECO:0000313|EMBL:EFN81707.1, ECO:0000313|Proteomes:UP000008237}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R22 G/1 {ECO:0000313|EMBL:EFN81707.1,
RC ECO:0000313|Proteomes:UP000008237};
RX PubMed=20798317; DOI=10.1126/science.1192428;
RA Bonasio R., Zhang G., Ye C., Mutti N.S., Fang X., Qin N., Donahue G.,
RA Yang P., Li Q., Li C., Zhang P., Huang Z., Berger S.L., Reinberg D.,
RA Wang J., Liebig J.;
RT "Genomic comparison of the ants Camponotus floridanus and Harpegnathos
RT saltator.";
RL Science 329:1068-1071(2010).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; GL449965; EFN81707.1; -; Genomic_DNA.
DR STRING; 610380.E2BRL5; -.
DR InParanoid; E2BRL5; -.
DR OMA; NREDYQQ; -.
DR Proteomes; UP000008237; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0031981; C:nuclear lumen; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 1.10.357.120; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000008237};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 285..620
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1349..1469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1349..1413
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1414..1429
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1687 AA; 193021 MW; 53204866BD298F9E CRC64;
MFSVFTANDI RNLSVTKIRT PLSFNILGHP LKGGLYDPVL GPLMGNSEPC GTCGGNVHTC
TGHFGHIELS MPVVNPLFHK VLCMLIKLSC LSCFTFQIPP YVKLLLSAKW KLLYEGQLSD
IDNLDQEVTS TVAEIPNVLE AQTEYIRDFI DNYIQNIYNR DRFSCGVPQN HENQMNTKNI
NMQWHMYVDN ILKQYKAVKI CINCHEVIPK ISVMQNKIMT TKLNNSKDSA PKEMVSSTVV
HKLETVMIMP DQSKEYLRKL WNNEKDFLKL IIPCLDLMDI EYPTDVFFLE VIPVLPPIVR
PVNILHGQVI EHPQTQVYKN IMQDCLILRN IIQTIQDGGT DQLPQEGRLV FEQIRGLTAI
EKLHNAWQGL QVNVDHLMDR EMSKTSESVH CQGLKQIIEK KEGIIRMHMM GKRVNFAARS
VITPDPNLNI DEIGIPEAFA LKLTYPVPVT PWNVVELRRL ILNGPDVHPG AVMVENEDGM
IQAISSNDST KREAVAKRLF TTNSRTNKFF SGIKVIYRHL QNGDVLLLNR QPTLHKPSIM
AHKARILKGE KTLRLHYANC KAYNADFDGD EMNAHFPQNE LARSESYNIV SVSNQYLVPK
DGTPLSGLIQ DHMVSGVRLT SRGRFFSRAD YTQLVYTALS MKQGDIILLP PAIIKPKLQW
SGKQILSTVI INIIPGNKAR INLTSNTKIS LKEWQVNEPR RWKCGKEFDD PKTMSEMEVI
IRHGELLCGV LDKMHYGATP YGLIHCIYEL YGGTCASKML SAFGKLFQAF LQWNGFTLGI
EDILITREAD EKRKEIISNS KIIGSSIQRS AVELSDDTPM DEVRVKTEES YWSNSKFRAQ
VDRKYKNMLD VYTNDINKTC LPMGLSKKFP DNNLQLMVQS GAKGSTVNTM QISCLLGQIE
LEGKRPPLMI SGKSLPSFQA YDSTPRAGGY IVGRFMTGIK SQEFFFHCMA GREGLIDTAV
KTSRSGYLQR CLIKHLEGLV INYDSTVRDS DGSLIQFYYG EDGLDIPGSR FLKKEQIPFL
VDNKDAIVDA EALENLKTGS SNNEKIMKLI KKIKKWKIKN GPPLQKRRIS EFSKFSMENV
NQNNNTKKNS VEKNCGRTKA ALSLIKKWMK IDKEMKKMFH IQCVRCPDPI MSKYRQDVEF
GVLSERLESL LEEYLSHSPN IIKKSLRDLL SMKVMKTICP PGEPVGLLAA QSIGEPSTQM
TLNTFHFAGR GDMNVTLGIP RLREILMMAS KNIKTPSMEI PFKSNLQKLT KQSKKLRVKL
TKCVLSDVLK IITISRKMEE APHRRLVHTI KIEFLPYKYY KGEFFVHPQY VIKQTEKTFF
KNIFKEIKKI AKITGTLLYF EENNRKRTNN EDAMLDQADP DEEESVRRER REDMRGERDL
GEMHESSDEE RAAEDADATM NRSISRHREN QEYEDPEEEE MEDVMNNENE EADNNANKDL
GNNQFDEDDD DDDDDDMTEN LNYRKNDKRN SNRRSEVIHM YKNALDYDYD ENKFSWCQFT
FWLPLKMIKL DLPTIIRNVA SKTVLWETPY IKKAFTFQNN EGEMILKTDG LNIVEMFKYD
SILNLNKLYS NDIYNISQTY GIEAANRVII KEIRDVFKMY GITVDSRHLS LIADYMTFDG
TFQPLSRKGM ENSSSPLQQM SFESSLNFLK TATLQGKKDD LLSPSSRLML GQPCKSGTGI
CSLFVNI
//
