ID E3EJJ5_PAEPS Unreviewed; 493 AA.
AC E3EJJ5;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN Name=celA1 {ECO:0000313|EMBL:ADO56545.1};
GN ORFNames=PPSC2_12105 {ECO:0000313|EMBL:ADO56545.1};
OS Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa).
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=886882 {ECO:0000313|EMBL:ADO56545.1, ECO:0000313|Proteomes:UP000006868};
RN [1] {ECO:0000313|EMBL:ADO56545.1, ECO:0000313|Proteomes:UP000006868}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC2 {ECO:0000313|EMBL:ADO56545.1,
RC ECO:0000313|Proteomes:UP000006868};
RX PubMed=21037012; DOI=10.1128/JB.01234-10;
RA Ma M., Wang C., Ding Y., Li L., Shen D., Jiang X., Guan D., Cao F.,
RA Chen H., Feng R., Wang X., Ge Y., Yao L., Bing X., Yang X., Li J., Du B.;
RT "Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant
RT growth-promoting Rhizobacterium with broad-spectrum antimicrobial
RT activity.";
RL J. Bacteriol. 193:311-312(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; CP002213; ADO56545.1; -; Genomic_DNA.
DR RefSeq; WP_013371149.1; NC_014622.2.
DR AlphaFoldDB; E3EJJ5; -.
DR STRING; 1406.LK13_24440; -.
DR CAZy; CBM59; Carbohydrate-Binding Module Family 59.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR KEGG; ppm:PPSC2_12105; -.
DR PATRIC; fig|886882.15.peg.2560; -.
DR eggNOG; COG2730; Bacteria.
DR eggNOG; COG4124; Bacteria.
DR HOGENOM; CLU_012932_1_0_9; -.
DR OrthoDB; 9800955at2; -.
DR Proteomes; UP000006868; Chromosome.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR049475; Mann_GBD_bact.
DR PANTHER; PTHR34142:SF1; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR Pfam; PF00150; Cellulase; 1.
DR Pfam; PF21253; Mann_GBD_bact; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023326};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361153};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Reference proteome {ECO:0000313|Proteomes:UP000006868};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..36
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 37..493
FT /note="cellulase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003169010"
FT DOMAIN 61..299
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
FT DOMAIN 347..489
FT /note="Mannanase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21253"
SQ SEQUENCE 493 AA; 53519 MW; 0D257FC41CD1B686 CRC64;
MKMSFNSFTK PLIMILLFSL LTLLFQAALP ATPASAAACA NPVSYFGEMK ASGNKINGSK
TNRPMMVKGS SFFWSNWSGP FWNTATVNRM VDEFQVEIVR AAYGVDPSGN PYNTADESKV
RDVVNAAIAK GIYVIIDWHS HDAHNNVNAA KSFFSRMAQE YGSYDNVIFE IYNEPTQVSW
GTIKSYAEQV IPAIRQHSDN LIVVGTPFWS QNVDQAANDP ITSSSNIAYT LHFYAGTHFQ
SLRDKANYAM SKGIPLFVTE MGFVNADGDG GINYDSTNQW LDWMNQNNLS WANWAINDKT
EGASIFNTNG SLTAGGNYLK SILAGHAPYA EWRQNAACGG SSANTLYDFE GSTQGWTGSN
ISDGPWSVNE WSSKGSNSLK ADIIMSANSQ HYLLLSQSRN LSGKATLSAT VKHASWDSAG
DGLNAKIYAK TGSGWAWYAG KAVKINSSGG TTLSFNLASV PNLDDVREIG IQFLASANSS
GKSAVYVDNV TLQ
//
