ID E3JWM7_PUCGT Unreviewed; 644 AA.
AC E3JWM7;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE SubName: Full=Methylenetetrahydrofolate reductase (NADPH) {ECO:0000313|EMBL:EFP76452.1};
GN ORFNames=PGTG_02893 {ECO:0000313|EMBL:EFP76452.1};
OS Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)
OS (Black stem rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=418459 {ECO:0000313|EMBL:EFP76452.1, ECO:0000313|Proteomes:UP000008783};
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CRL 75-36-700-3;
RG The Broad Institute Genome Sequencing Platform;
RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Cuomo C., Jaffe D.,
RA Butler J., Alvarez P., Gnerre S., Grabherr M., Mauceli E., Brockman W.,
RA Young S., LaButti K., Sykes S., DeCaprio D., Crawford M., Koehrsen M.,
RA Engels R., Montgomery P., Pearson M., Howarth C., Larson L., White J.,
RA Zeng Q., Kodira C., Yandava C., Alvarado L., O'Leary S., Szabo L., Dean R.,
RA Schein J.;
RT "The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-
RT 700-3.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000008783}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CRL 75-36-700-3 / race SCCL
RC {ECO:0000313|Proteomes:UP000008783};
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU004254}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743}.
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DR EMBL; DS178265; EFP76452.1; -; Genomic_DNA.
DR RefSeq; XP_003320871.1; XM_003320823.2.
DR AlphaFoldDB; E3JWM7; -.
DR STRING; 418459.E3JWM7; -.
DR EnsemblFungi; EFP76452; EFP76452; PGTG_02893.
DR GeneID; 10534508; -.
DR KEGG; pgr:PGTG_02893; -.
DR VEuPathDB; FungiDB:PGTG_02893; -.
DR eggNOG; KOG0564; Eukaryota.
DR HOGENOM; CLU_025841_2_2_1; -.
DR InParanoid; E3JWM7; -.
DR OMA; VARTSQW; -.
DR OrthoDB; 1381745at2759; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000008783; Unassembled WGS sequence.
DR GO; GO:0071949; F:FAD binding; IBA:GO_Central.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IBA:GO_Central.
DR GO; GO:0009086; P:methionine biosynthetic process; IBA:GO_Central.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IBA:GO_Central.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR004621; Fadh2_euk.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR NCBIfam; TIGR00677; fadh2_euk; 1.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000008783}.
FT REGION 608..632
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..632
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 644 AA; 72636 MW; CCE9B55DB0834E44 CRC64;
MKISQKLAAA ESENRISWSF EYFPPKTDNG LTNLYDRICR MSQLGPIFID ITWGAGGSTS
NLTTEFVKTA HEQFGLETCM HLTCTNMPKS KIDTALKEAY EFGCENILAL RGDPPKGSTE
WTATEGGFEH AIDLVRHIRA EYGDHFDIAV AGFPEGHMHS TLSREQQTIY LKEKIEAGAN
FIFTQMFYDV DIFIDWVREI RAAGITVPVI PGLMPIQSWA QFKRVTNFSK TIVPQYFLDK
LEPIQSDDQA VREAGTKLVA DMCRKILNSD LGIRILHFYT MNLERGTKMI LDELNLNPSR
DITNPLPWKM SLLGKRRSES IRPIFWANRA KSYLSRTETW DEFPNGRWGD SRSPAYGELD
GYGININAAT METLKGLKKF PTSLADLARI FANYCGARFP GEEPVKYLPW SDIPLSEETN
KIEDVLVKIN KRGFLTINSQ PAVDGAKSSD PVHGWGPRGG YVYQKAYLEF FCCPEKFQSL
TERLEKDPDV TYYAVTKTGD LVTNNTRPGP NAVTWGVFPG KEIVQPTIVE LVSFIAWKDE
AFELGHQWAE LYKEIAPESA RFIHQIMDSW YLVNIVNNDF RKSNDDSEHS ALFQFFGPEP
VIVDQDDLPP TTNGLSHPNG LPKTNGSSTP AISEVNGELK NLAI
//
