ID E3KQM4_PUCGT Unreviewed; 1306 AA.
AC E3KQM4;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 2.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=PGTG_12981 {ECO:0000313|EMBL:EFP86599.2};
OS Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)
OS (Black stem rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=418459 {ECO:0000313|EMBL:EFP86599.2, ECO:0000313|Proteomes:UP000008783};
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CRL 75-36-700-3;
RG The Broad Institute Genome Sequencing Platform;
RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Cuomo C., Jaffe D.,
RA Butler J., Alvarez P., Gnerre S., Grabherr M., Mauceli E., Brockman W.,
RA Young S., LaButti K., Sykes S., DeCaprio D., Crawford M., Koehrsen M.,
RA Engels R., Montgomery P., Pearson M., Howarth C., Larson L., White J.,
RA Zeng Q., Kodira C., Yandava C., Alvarado L., O'Leary S., Szabo L., Dean R.,
RA Schein J.;
RT "The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-
RT 700-3.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000008783}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CRL 75-36-700-3 / race SCCL
RC {ECO:0000313|Proteomes:UP000008783};
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR EMBL; DS178301; EFP86599.2; -; Genomic_DNA.
DR RefSeq; XP_003331018.2; XM_003330970.2.
DR STRING; 418459.E3KQM4; -.
DR EnsemblFungi; EFP86599; EFP86599; PGTG_12981.
DR GeneID; 10540699; -.
DR KEGG; pgr:PGTG_12981; -.
DR VEuPathDB; FungiDB:PGTG_12981; -.
DR HOGENOM; CLU_261088_0_0_1; -.
DR InParanoid; E3KQM4; -.
DR OrthoDB; 1611579at2759; -.
DR Proteomes; UP000008783; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR Pfam; PF05970; PIF1; 2.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000008783}.
FT DOMAIN 1161..1204
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
FT REGION 100..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 195..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 483..524
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 628..732
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..243
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..498
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 628..692
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 705..732
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1306 AA; 143093 MW; E979A5DDA60B66F8 CRC64;
MADNGGGSSS DAMVKIKPQN EDLAFNGSNV KRFLSEYQLA ARLDGASEKD MAQQLGFFIA
KDKLLDVVET LEGYEPPDWP KLKASMIAYW GNVDTTKFSL PDPVPSDQSL EEENPQNLER
GLPLGSHNDL LVEEALESDS NFVDLDVGPE LFDKSSKQDR NESIKTREPA WHPTNTSLYF
TAASAIEYPL ASRINKSNPS RRQHENLKSS TVLSVSLPPV KDSTLPVPDS DQEDMDDNPE
LFEQSTEDPS PAEVTAARLI LALNPVASPI NNHLSTEISE NLSSASASAA TKDKEKFLVD
QKGHNYFLPS GAWIPFDPVR PIRSVVTAFQ ASSRSAPPFP APYRISCGAL QPWYPTTKPI
PCADPPVSES YHCHLIRKSP NIKSSQPKVL SSDPPSPKCL AKFVKEANSV LKKIINLMVP
GFSASSLDNV HLPKSQIPRR TKARKVIPRR HNGVKVSSLQ YAPPGLDATK IVKTLSRATL
VSNGQPSALD SPGVPDPTSS ETLRDEVKLS SDPPLCSVPD LDPIGQLRPT SERMVSFPIA
LRDEVKLSSD HSLHSVPNLD PIGQLRPTSE RLVPSSIALQ NEVKLSSDPS LCSVPDLDPI
GQLRPTAESL VSSSIRLHKE ATLNSQVQES VFSSQASSDP NNHMSSLSHN YQSHPSGVPK
SSITPEVHQS QSSQPLSNSV DPLSSSSPIR LRNEDKPASV PIPNPLRNET KQTSESSNVE
YPDPVSSDGV QASGQVENDL CNEGELGLLH FDEEEGWMIE VFPRGGKVDQ NPPSRPQGLL
EEFIEVLSDN LIYKLQNTYN IRNPSLEQRT SLCYYLIKQI LAENGKSLAD VGLRPIAADD
RLWWYFDTVS AREETMRITD HTNRFLEMSA RLNQKQSAIA DAVIGLTSAG EAGLIYVDGP
GGCGKTFLLN TLIHYFNASE IPVITVASSG VASLMLINGM TAHSRFKIPL NVKYAVEAVD
QAFRSLMDNE QPFGGKIVIF GGDFRQTLPV VPGGSVLDQG RCCMISSAIW NDVFYFQLTQ
NLRLRASGDD QSARSNLLRP TGFAEWLLSV GNGSGQTDFT ADIDIAFGWV YRHSDARVVA
ERAIVKTYGD MAVALNDPEA GRLNAYFGNR LILAPLNSDV NRINAICSSR LPGEVFVSHS
INQMRNEEDG EDSDEAIPEE VLRTFSIPGF PEADIELKVG MPVILLRNLD LKRGLSNGTR
LLVLAIRPDA LRCRILTGSC TGAEIAIPRI RHGPKYSFAS TRYLFSSTCW ECTSTRPRVA
GAKVQVTGLT TNPRSHWSHA HRQTHPSNRE VLFIAPSNPD QPHPPR
//
