ID E3L6E6_PUCGT Unreviewed; 1125 AA.
AC E3L6E6;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=PGTG_17426 {ECO:0000313|EMBL:EFP92121.1};
OS Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)
OS (Black stem rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=418459 {ECO:0000313|EMBL:EFP92121.1, ECO:0000313|Proteomes:UP000008783};
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CRL 75-36-700-3;
RG The Broad Institute Genome Sequencing Platform;
RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Cuomo C., Jaffe D.,
RA Butler J., Alvarez P., Gnerre S., Grabherr M., Mauceli E., Brockman W.,
RA Young S., LaButti K., Sykes S., DeCaprio D., Crawford M., Koehrsen M.,
RA Engels R., Montgomery P., Pearson M., Howarth C., Larson L., White J.,
RA Zeng Q., Kodira C., Yandava C., Alvarado L., O'Leary S., Szabo L., Dean R.,
RA Schein J.;
RT "The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-
RT 700-3.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000008783}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CRL 75-36-700-3 / race SCCL
RC {ECO:0000313|Proteomes:UP000008783};
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR EMBL; DS178358; EFP92121.1; -; Genomic_DNA.
DR RefSeq; XP_003336540.1; XM_003336492.1.
DR AlphaFoldDB; E3L6E6; -.
DR STRING; 418459.E3L6E6; -.
DR EnsemblFungi; EFP92121; EFP92121; PGTG_17426.
DR GeneID; 10532081; -.
DR KEGG; pgr:PGTG_17426; -.
DR VEuPathDB; FungiDB:PGTG_17426; -.
DR HOGENOM; CLU_279793_0_0_1; -.
DR InParanoid; E3L6E6; -.
DR OMA; DETFHMH; -.
DR OrthoDB; 1411896at2759; -.
DR Proteomes; UP000008783; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0043596; C:nuclear replication fork; IBA:GO_Central.
DR GO; GO:0043139; F:5'-3' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0044806; P:G-quadruplex DNA unwinding; IBA:GO_Central.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IBA:GO_Central.
DR GO; GO:0071932; P:replication fork reversal; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR23274:SF49; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR23274; DNA HELICASE-RELATED; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000008783}.
FT DOMAIN 928..972
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 1125 AA; 125334 MW; 4E9E4B9A3731FD92 CRC64;
MTKNIDIGSL VSEPTKLYGL EPTLEGIGEL TFGGTCSLEE KFQSFDLDMG LCNIYKIQLP
IRPELQDCLR CVYASDEKII AALIAPCLED LGCSLSLFTD YTIRGSISHN DTSGTVWEFN
PLLVRLCDSV AGQARQHGMD IGGVGIVQSI EERWDLAKTK HTKDEILVNH QPSKIQVLHT
KTEMINSKSK ITNSTSPDFE SRLHNASDYS VPHRLGRCNH QCDSCGALHW AAEATLVDRT
KLNKSYSMCC QKNKVTLPDF HESAPKYPSI LRRLLTGKGE EEVNFQQFTR MYNNSISFTS
LGARIDRSVQ GPMGVNIFKI SGALSHRISS LEPDNNSPGF SQIYVVGNKG IEEAEYRLNC
ALGQKKDNVK GVKIRQGIIL SLINLMEKIN PYANIFRNDL EVLSKAKAET ISLQGVPSAG
VDPKRYNNPT VDEDRQVGSL EWFAYLLFRR RKRFSPILAD SNDEVRSFVD GRYISAVEVT
CLAVHDEDEQ LVYFEGEDGL AGQIESGKAN QTTLTGYFEL NAKNALGADD VPARSIFYED
IPAYFSWNKA DKRWIRRKKR ALAIGQVFSI SYLAGERFYL RVLLLHRKGM VSFEDLKTVD
GKVSQSYQDA CNDRQLNEDE RRVLAIFRLK TFVEGMGTTL DSCGLTITKK EKRLLREIQA
EQEGSKNPNN IAARLCGSSA SQDGPNSSLN IQNTDSCTSW WRMYSGGGHI AGEKLIAARL
IIWDEIVTIH KNSIDAVNKS LKRICNSDAE FGGKDAIFLG DFRQILPVVK YNEHPPAFAA
TIKSSKIWPS IRQFKLTENM RLAQAIGASS DDKNRVFARA LLILGEGKKQ KSDFSIVTLN
HMNIEAFKSK DEMRQELIEF VYGKLASQGQ RTNREKIAYL NERCILAPLN RDVKKLNLEI
LSRLPGVTTT LRSIDTPDPD GVGSLPEECL NKLSIGGFPD HELQIKIGMP LVVLRNMDIK
NGVCNGSRIM VIAYGVGFIV GKLMSGPCAG NEITLPRAKL HNKSNARSGL SFFRYQFPVA
PAYAMSVNKS QGQTLGKVGV YLETDVFSHG QLYVAVSRVS DVANLLIVRP ANRLGILNVV
HRGLFKMCRK REVSKAICNL NGGEVEDFQF SVEVENTVPV MGNIN
//
