ID E3RTQ6_PYRTT Unreviewed; 1079 AA.
AC E3RTQ6;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 24-JAN-2024, entry version 60.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=PTT_12410 {ECO:0000313|EMBL:EFQ90883.1};
OS Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus)
OS (Drechslera teres f. teres).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae;
OC Pyrenophora.
OX NCBI_TaxID=861557 {ECO:0000313|Proteomes:UP000001067};
RN [1] {ECO:0000313|EMBL:EFQ90883.1, ECO:0000313|Proteomes:UP000001067}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0-1 {ECO:0000313|EMBL:EFQ90883.1,
RC ECO:0000313|Proteomes:UP000001067};
RX PubMed=21067574; DOI=10.1186/gb-2010-11-11-r109;
RA Ellwood S.R., Liu Z., Syme R.A., Lai Z., Hane J.K., Keiper F., Moffat C.S.,
RA Oliver R.P., Friesen T.L.;
RT "A first genome assembly of the barley fungal pathogen Pyrenophora teres f.
RT teres.";
RL Genome Biol. 11:R109.1-R109.14(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; GL535021; EFQ90883.1; -; Genomic_DNA.
DR RefSeq; XP_003301011.1; XM_003300963.1.
DR AlphaFoldDB; E3RTQ6; -.
DR STRING; 861557.E3RTQ6; -.
DR EnsemblFungi; EFQ90883; EFQ90883; PTT_12410.
DR KEGG; pte:PTT_12410; -.
DR eggNOG; KOG0434; Eukaryota.
DR HOGENOM; CLU_001493_1_0_1; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000001067; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:EnsemblFungi.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000001067}.
FT DOMAIN 12..636
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 691..846
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1079 AA; 124248 MW; 8FDDC858F2AC8969 CRC64;
MSIDFPREEE RILARWKEID AFLRQVELSK GKQPYTFYDG PPFATGMPHY GHLLASTIKD
IIPRYWSMKG RYVERRFGWD THGVPIEYEI DKELGMSGRD AVREIGIAKY NEKCRAIVMR
YATEWRATID RLGRWIDFDN DYKTMDTSFM ETEWWVFKRL FDKGAVYRGF KVMPYSTALA
TPLSNFEASQ NYKDVQDPAV VVTFPLVEDP NTCLLAWTTT PWTLPSNTGL CAHPDFEYVK
ILDEASGKHY ILLESLMKTL YKDPKKAKFK IVEKIKGKDM LGWKYEPLFD YLYDEFKDYG
FKVLNDTYVT ADGGVGIVHQ APAYGEDDYR VALAHGVISD TRTPPNPVDD QGRFTAVVRD
FEGQHVKAAD KGIIKHLKGT GRLIVDSQLL HSYPFCWRSD TPLIYRTVPS WFIKIQDIVP
QMLENIAGSH WVPSFVKEKR FANWITNSPD WAVSRNRFWG TPLPIWVSDD GKEVVCIGSV
EELRKLSGYE GELTDIHRDK IDHITIPSKD GKGVLRRTNE VFDCWFESGS MPYASAHYPF
ENVEQFEKSF PGDFIAEGLD QTRGWFYTLT VLGTHLFGKL PFKNCVVNGI VLAEDGKKMS
KRLKNYPDPT LIMNSYGSDA LRLYLINSPV VRAETLRFKE AGVKEIVSKV LLPLWNSYQF
FEQQVTLLKK VADLDFVFDP AAGKTNANVL DRWILASCQS LLKFVNEEMA AYRLYTVVPR
LLELIDNTTN WYIRFNRRRL KGEHGLEDTK HALNTLFEVL YTLCRGLAPF TPFITDNIYL
RLLPHIPKEL HAEDNRSIHF LPFPEVREEL FDEAVERQVK RMQSVIELGR ICRDRANKGL
KIPLKTLVVI HPEQQYLDDI KSLENYILEE LNVRDIVLSS DEEKYNVQYS ASADFSVLGK
KLKKDAIKVK KALPNLTSQE IKDYLKSGEI VVDGIKLEAE DLMVKRGLAK DSGNKDQEFN
TDNDVLIILD VASYPELEQE GLAREVIRRV QDLRKKAGLV PTDDVGMEYR VLSDPENVGL
EKAFENQGPL FEKALRRNVD KHVITEVEGK MPENSNEKVI AEEEQELHKA TFMLRLIKL
//
