ID E4RJ41_HALHG Unreviewed; 736 AA.
AC E4RJ41;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 67.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=Halsa_1843 {ECO:0000313|EMBL:ADQ15261.1};
OS Halanaerobium hydrogeniformans (Halanaerobium sp. (strain sapolanicus)).
OC Bacteria; Bacillota; Clostridia; Halanaerobiales; Halanaerobiaceae;
OC Halanaerobium.
OX NCBI_TaxID=656519 {ECO:0000313|EMBL:ADQ15261.1, ECO:0000313|Proteomes:UP000007434};
RN [1] {ECO:0000313|EMBL:ADQ15261.1, ECO:0000313|Proteomes:UP000007434}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=sapolanicus {ECO:0000313|Proteomes:UP000007434};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M.,
RA Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA Begemann M.B., Mormile M.R., Wall J.D., Elias D.A., Woyke T.;
RT "Complete sequence of Halanaerobium sp. sapolanicus.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADQ15261.1, ECO:0000313|Proteomes:UP000007434}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=sapolanicus {ECO:0000313|Proteomes:UP000007434};
RX PubMed=21602336; DOI=10.1128/JB.05209-11;
RA Brown S.D., Begemann M.B., Mormile M.R., Wall J.D., Han C.S., Goodwin L.A.,
RA Pitluck S., Land M.L., Hauser L.J., Elias D.A.;
RT "Complete Genome Sequence of the Haloalkaliphilic, Hydrogen Producing
RT Halanaerobium hydrogenoformans.";
RL J. Bacteriol. 193:3682-3683(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP002304; ADQ15261.1; -; Genomic_DNA.
DR RefSeq; WP_013406332.1; NC_014654.1.
DR AlphaFoldDB; E4RJ41; -.
DR STRING; 656519.Halsa_1843; -.
DR KEGG; has:Halsa_1843; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_362043_0_0_9; -.
DR OrthoDB; 9765670at2; -.
DR Proteomes; UP000007434; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000007434}.
FT DOMAIN 3..317
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 24..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 736 AA; 84306 MW; 9314902C697D6ADD CRC64;
MDFKARPGQK EVLEYRGGQL AVPAVPGAGK TTVLAHLAAD LITEELNSGQ KILIVTYMNS
AVANFRKMIG NCLAAKGLPR SRGYSVKTLH SLALEIIKEK PEARLINQDF ELIESGRRFR
WIKQLSSKWA AENNDLLLDY FNLEKNSYHF KKYSKRWKDE DFPAYVSSMI SYFKLKLLEA
DELREMIRYS NLRKRNILTP AADIYAEYQF KLAQSGLLDF DDLVQHSLKL LKKDPDFAER
ISAQYSYVFE DEAQDSNQAQ EEMLYLLAGE NKNLLRVGDS NQAITGSFTL SDPEIFRNYC
RSDEVKVQTM SVSSRSSQDI IDLANFLVDW SQSEFENRNL DALEKKYIEP VLEDDPVSNP
EPEGYRIGIR SFKNWKEEKK FLARQAFRKS QENSNKTTAV LMPIGRQLEE IGAEINKLGG
EYQLAGSTAS NVFLTEIEVI LKFLTSPNSA NNLKLLLNEV FLEDDQQARQ LLEELLEEKE
VAEIIFPTAG LSRDIFNITL RKKEGFNEIF KAVTKLQGWL EASMEIPPDE LVLFLAERIE
LSGKKLALAQ NLSLEFGRLL KENPAWRLDQ LTAELPEIRD RLKQFAETLT EMEGFETAPD
KVSLSTMHKA KGMEWDTVFI GGLTSDQFQH SDDDYFMGEK FYLSEEIKNP KAAARAELEY
LINDKKEKNA DKKARQQLIA ERIRLLYVAI TRAKENLFLS SHKKRGSWDK SPAYVFSDLK
DFINEKQKGG SDRNGK
//
