ID E4RKI2_HALHG Unreviewed; 793 AA.
AC E4RKI2;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN OrderedLocusNames=Halsa_1263 {ECO:0000313|EMBL:ADQ14691.1};
OS Halanaerobium hydrogeniformans (Halanaerobium sp. (strain sapolanicus)).
OC Bacteria; Bacillota; Clostridia; Halanaerobiales; Halanaerobiaceae;
OC Halanaerobium.
OX NCBI_TaxID=656519 {ECO:0000313|EMBL:ADQ14691.1, ECO:0000313|Proteomes:UP000007434};
RN [1] {ECO:0000313|EMBL:ADQ14691.1, ECO:0000313|Proteomes:UP000007434}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=sapolanicus {ECO:0000313|Proteomes:UP000007434};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M.,
RA Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA Begemann M.B., Mormile M.R., Wall J.D., Elias D.A., Woyke T.;
RT "Complete sequence of Halanaerobium sp. sapolanicus.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADQ14691.1, ECO:0000313|Proteomes:UP000007434}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=sapolanicus {ECO:0000313|Proteomes:UP000007434};
RX PubMed=21602336; DOI=10.1128/JB.05209-11;
RA Brown S.D., Begemann M.B., Mormile M.R., Wall J.D., Han C.S., Goodwin L.A.,
RA Pitluck S., Land M.L., Hauser L.J., Elias D.A.;
RT "Complete Genome Sequence of the Haloalkaliphilic, Hydrogen Producing
RT Halanaerobium hydrogenoformans.";
RL J. Bacteriol. 193:3682-3683(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. {ECO:0000256|ARBA:ARBA00008263}.
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DR EMBL; CP002304; ADQ14691.1; -; Genomic_DNA.
DR RefSeq; WP_013405772.1; NC_014654.1.
DR AlphaFoldDB; E4RKI2; -.
DR STRING; 656519.Halsa_1263; -.
DR KEGG; has:Halsa_1263; -.
DR eggNOG; COG0188; Bacteria.
DR HOGENOM; CLU_002977_6_1_9; -.
DR OrthoDB; 9806486at2; -.
DR Proteomes; UP000007434; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 3.30.1360.40; -; 1.
DR Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR NCBIfam; TIGR01063; gyrA; 1.
DR PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR Pfam; PF03989; DNA_gyraseA_C; 3.
DR Pfam; PF00521; DNA_topoisoIV; 1.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:ADQ14691.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000007434};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 8..461
FT /note="DNA topoisomerase type IIA"
FT /evidence="ECO:0000259|SMART:SM00434"
FT COILED 433..481
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 793 AA; 89954 MW; F3D3CDE50FAE77C5 CRC64;
MSEEIYPIAI EDKMKDAYLN YSLSVIVGRA LPDVRDGLKP VHRRILYAAR ELGLLHNKAH
KKSARLVGEV LGKFHPHGDS AVYDALVRMA QDFNQRYQLI DGHGNFGSID GDSAAAMRYT
ETKLTEVAES LLENIDEETV KFRDNFDGTL QEPEVLPSKI PNLLINGSSG IAVGMSTSIP
PHNLAEVIDA VLHLLEYPNA RLDTIIKNHL PGPDFPTGAS IVGNEGIKSA YENGKGKIIL
RAKSKIEKEG RHKNIIITEI PYQLNKSKLI EEIAELVNKS KLTSVSDLRD ESDKDGIRIL
IALKSGADPE LTLNRLYKYT SMQSSFRINL LALDNKKPKV MGLLEILNHF IEFRKEVIKN
HTKYNLNKSE NRFHILEGLK IAINKLDLVI QIIRNSNEKS EAKAKLVEQL NISEVQAQSI
LQMQLQRLVG MEIEKLEAEK TELAAKIKEY KKILTEEKTL KEYLKNELKE MKDKFADKRR
TTIIEDDEKA EINKQDLIKE KEAVVSLSYR GIIKRNEEYS NLRAAKNDYL ISTVKGSSLD
QLLFFSDHGE VYNLQVHELE EHHGLATGDN LSKYMQLPLE ENIISLNILS KKNKQNNFYV
FVTESGQIKR TAAKEYETNY SHIRAIKLDN DKLINVEKAE EKDQIMIISQ NGYAIRFKGK
SFSSTGRNTM GSKAIKLAKN DKVTAFSIFN KNKYLVSVTE TGRANKIKLS QFRLQKRNGR
GQRIFSNQNY KLAGAAFCEE NEKILIAAKN GEMYTVEVDK IPETSPGGNM YQITKNIDFE
KITRVKKILI PDD
//
