ID E4U615_OCEP5 Unreviewed; 214 AA.
AC E4U615;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=tRNA (guanosine(18)-2'-O)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_02060};
DE EC=2.1.1.34 {ECO:0000256|HAMAP-Rule:MF_02060};
DE AltName: Full=tRNA [Gm18] methyltransferase {ECO:0000256|HAMAP-Rule:MF_02060};
GN Name=trmH {ECO:0000256|HAMAP-Rule:MF_02060};
GN OrderedLocusNames=Ocepr_0376 {ECO:0000313|EMBL:ADR35836.1};
OS Oceanithermus profundus (strain DSM 14977 / NBRC 100410 / VKM B-2274 /
OS 506).
OC Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Oceanithermus.
OX NCBI_TaxID=670487 {ECO:0000313|EMBL:ADR35836.1, ECO:0000313|Proteomes:UP000008722};
RN [1] {ECO:0000313|Proteomes:UP000008722}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14977 / NBRC 100410 / VKM B-2274 / 506
RC {ECO:0000313|Proteomes:UP000008722};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Zhang X., Brettin T.,
RA Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V.,
RA Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Tindall B., Faehnrich R.,
RA Brambilla E., Klenk H.-P., Eisen J.A.;
RT "The complete sequence of chromosome of Oceanithermus profundus DSM
RT 14977.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADR35836.1, ECO:0000313|Proteomes:UP000008722}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14977 / NBRC 100410 / VKM B-2274 / 506
RC {ECO:0000313|Proteomes:UP000008722};
RX PubMed=21677858; DOI=10.4056/sigs.1734292;
RA Pati A., Zhang X., Lapidus A., Nolan M., Lucas S., Del Rio T.G., Tice H.,
RA Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Liolios K.,
RA Pagani I., Ivanova N., Mavromatis K., Chen A., Palaniappan K., Hauser L.,
RA Jeffries C.D., Brambilla E.M., Rohl A., Mwirichia R., Rohde M.,
RA Tindall B.J., Sikorski J., Wirth R., Goker M., Woyke T., Detter J.C.,
RA Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C.,
RA Klenk H.P., Land M.;
RT "Complete genome sequence of Oceanithermus profundus type strain (506).";
RL Stand. Genomic Sci. 4:210-220(2011).
CC -!- FUNCTION: Catalyzes the 2'-O methylation of guanosine at position 18 in
CC tRNA. {ECO:0000256|HAMAP-Rule:MF_02060}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-
CC methylguanosine(18) in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:20077, Rhea:RHEA-COMP:10190, Rhea:RHEA-COMP:10192,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74445; EC=2.1.1.34;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_02060};
CC -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC superfamily. RNA methyltransferase TrmH family. {ECO:0000256|HAMAP-
CC Rule:MF_02060}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_02060}.
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DR EMBL; CP002361; ADR35836.1; -; Genomic_DNA.
DR RefSeq; WP_013457006.1; NC_014761.1.
DR AlphaFoldDB; E4U615; -.
DR STRING; 670487.Ocepr_0376; -.
DR KEGG; opr:Ocepr_0376; -.
DR eggNOG; COG0566; Bacteria.
DR HOGENOM; CLU_021322_4_2_0; -.
DR OrthoDB; 9794400at2; -.
DR Proteomes; UP000008722; Chromosome.
DR GO; GO:0141100; F:tRNA (guanine(18)-2'-O)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0002938; P:tRNA guanine ribose methylation; IEA:UniProtKB-UniRule.
DR CDD; cd18092; SpoU-like_TrmH; 1.
DR Gene3D; 3.40.1280.10; -; 1.
DR HAMAP; MF_02060; tRNA_methyltr_TrmH; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR022724; rRNA_MeTrfase_SpoU_C.
DR InterPro; IPR001537; SpoU_MeTrfase.
DR InterPro; IPR033671; TrmH.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR PANTHER; PTHR43453; RRNA METHYLASE-LIKE; 1.
DR PANTHER; PTHR43453:SF1; SPOU_METHYLASE DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF12105; SpoU_methylas_C; 1.
DR Pfam; PF00588; SpoU_methylase; 1.
DR SUPFAM; SSF75217; alpha/beta knot; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|HAMAP-
KW Rule:MF_02060}; Reference proteome {ECO:0000313|Proteomes:UP000008722};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_02060};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_02060};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_02060};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_02060};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_02060}.
FT DOMAIN 20..159
FT /note="tRNA/rRNA methyltransferase SpoU type"
FT /evidence="ECO:0000259|Pfam:PF00588"
FT DOMAIN 164..214
FT /note="RNA methyltransferase SpoU/TrmH type C-terminal"
FT /evidence="ECO:0000259|Pfam:PF12105"
FT BINDING 119..123
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02060"
FT BINDING 139
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02060"
FT BINDING 148
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02060"
SQ SEQUENCE 214 AA; 23947 MW; DBFA4BC26C7A9F48 CRC64;
MTPERYRRLR DVLDKRQPDL TVLMENVHKP HNLSAILRTA DAVGVYEAHA VNPTGGVPTF
HDTSGGSEKW VYLRVHPNVE AALEHLKGRG FTVYAANLSE RAVDYREVDY TRPSAVLLGA
EKWGVSPRAA ELADADVIIP MMGMVQSLNV SVAAAVILFE AQCQRLAAGL YARPRLDPET
YRRTLFEWAY PREAAVLRRQ GLPYPPLDEE GRIV
//
