ID E4ZNM6_LEPMJ Unreviewed; 658 AA.
AC E4ZNM6;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=Endo-1,5-alpha-L-arabinanase A {ECO:0000256|ARBA:ARBA00042202};
GN ORFNames=LEMA_P041460.1 {ECO:0000313|EMBL:CBX93245.1};
OS Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8)
OS (Blackleg fungus) (Phoma lingam).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Leptosphaeriaceae;
OC Plenodomus; Plenodomus lingam/Leptosphaeria maculans species complex.
OX NCBI_TaxID=985895 {ECO:0000313|Proteomes:UP000002668};
RN [1] {ECO:0000313|Proteomes:UP000002668}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8
RC {ECO:0000313|Proteomes:UP000002668};
RX PubMed=21326234; DOI=10.1038/ncomms1189;
RA Rouxel T., Grandaubert J., Hane J.K., Hoede C., van de Wouw A.P.,
RA Couloux A., Dominguez V., Anthouard V., Bally P., Bourras S.,
RA Cozijnsen A.J., Ciuffetti L.M., Degrave A., Dilmaghani A., Duret L.,
RA Fudal I., Goodwin S.B., Gout L., Glaser N., Linglin J., Kema G.H.J.,
RA Lapalu N., Lawrence C.B., May K., Meyer M., Ollivier B., Poulain J.,
RA Schoch C.L., Simon A., Spatafora J.W., Stachowiak A., Turgeon B.G.,
RA Tyler B.M., Vincent D., Weissenbach J., Amselem J., Quesneville H.,
RA Oliver R.P., Wincker P., Balesdent M.-H., Howlett B.J.;
RT "Effector diversification within compartments of the Leptosphaeria maculans
RT genome affected by Repeat-Induced Point mutations.";
RL Nat. Commun. 2:202-202(2011).
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865}.
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DR EMBL; FP929105; CBX93245.1; -; Genomic_DNA.
DR RefSeq; XP_003836610.1; XM_003836562.1.
DR AlphaFoldDB; E4ZNM6; -.
DR STRING; 985895.E4ZNM6; -.
DR CAZy; GH43; Glycoside Hydrolase Family 43.
DR EnsemblFungi; CBX93245; CBX93245; LEMA_P041460.1.
DR GeneID; 13287497; -.
DR eggNOG; ENOG502SM7B; Eukaryota.
DR HOGENOM; CLU_010779_2_0_1; -.
DR InParanoid; E4ZNM6; -.
DR OMA; MWSEDDT; -.
DR OrthoDB; 2719402at2759; -.
DR Proteomes; UP000002668; Genome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd08983; GH43_Bt3655-like; 1.
DR CDD; cd18828; GH43_BT3675-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 3.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:CBX93245.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002668};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..658
FT /note="Endo-1,5-alpha-L-arabinanase A"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003194798"
FT REGION 334..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 366
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 538
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 489
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 658 AA; 72218 MW; 0F8A7ABD70262D24 CRC64;
MFFTSIFSLL TLGAVSSVSA QAAVNEGYVF AYFTNNSRAG EKIYLAASNG NNALDWTELN
NGQPILTSTK GTTGLRDPFL IRSNDGSKFF LIATDLSIGS GTSWGAAVRT GSRYLEVWES
PDLRTWSEQR HILVSPPEAG NTWAPEAYFD TDLNEYVVFW ASSLYAANDP NHTGSTYHRM
LYATTKDFVT FSPTKIWQDA GTSRIDSTVL KAGDVFYRFT KDEGGVTGCA DIIQESSTSL
LAPLDGWTIG ASCIGRNAGL GAVEGPTAFK SNPGDVHGEK YYLFVDEYSG RGYVPLETAD
MSRPEWKVSP NYKLPASPRH GTVVPVTAAE LAGVTGRSTT NSRRKKSELA TRQASGNPVL
PDFYADPNAI IFDDTYYIYA TTDGFPGWGG KVFYTWQSKD LVNWSRAETP FLTLNGTNGN
VPWATGNAWA PTIIEREGTY SFYFSGHNPE FNRKTIGVAT ASSPEGPFTA QPTPMITNNE
AIKTGQAIDP HAFFDPATGK YWLSWGNGTP LMAELNDDMI SIKQETLHKV EGLVNFREGS
FLNYRKGLYH FTYSIDDTGV SGYRVGYATS EDIMGPWTYH GLILEKDESQ GILATGHSSI
INVPNTDEWY IVYHRFAIPG GDGTHRVTCI DKLTIDEETG LMQPVKPTLT GVDPRPLQ
//
