ID E6MGN5_9FIRM Unreviewed; 1178 AA.
AC E6MGN5;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=addA {ECO:0000313|EMBL:EFV01775.1};
GN ORFNames=HMP0721_1168 {ECO:0000313|EMBL:EFV01775.1};
OS Pseudoramibacter alactolyticus ATCC 23263.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Pseudoramibacter.
OX NCBI_TaxID=887929 {ECO:0000313|EMBL:EFV01775.1, ECO:0000313|Proteomes:UP000004754};
RN [1] {ECO:0000313|EMBL:EFV01775.1, ECO:0000313|Proteomes:UP000004754}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23263 {ECO:0000313|EMBL:EFV01775.1,
RC ECO:0000313|Proteomes:UP000004754};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFV01775.1}.
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DR EMBL; AEQN01000016; EFV01775.1; -; Genomic_DNA.
DR RefSeq; WP_006598592.1; NZ_GL622359.1.
DR AlphaFoldDB; E6MGN5; -.
DR STRING; 887929.HMP0721_1168; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000004754; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014152; AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02785; addA_Gpos; 1.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000004754}.
FT DOMAIN 1..461
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 478..765
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1178 AA; 133230 MW; 3FB53588E2278E1B CRC64;
MQWTTEQEAA ISSRHQNLLV SAAAGSGKTA LLIERIRRLV VDEDVDVDSL LVLTFTRAAA
GEMRERLSTA LSEALMACTD ADKRRRLSNQ LRRLSGASIE TIHAFCGDLV REFFQEVDAD
PNDRIGDDVE LDRLQDEAMT AVFEERYLEI PEDGETAFSK LVDAYGDSKG DEDLRTLVLD
LWRFLGTLKD PDSWCQKAID QLKVAPESFE ESAWGEAQKA SIRSLIESVC QEITQIQSMI
ADYPGFEKPM SSLQKDLDKL LDLKNHLADE WQVFQQALQT FKFDSYSGSK EDKETSNKIK
DLRTATIKPI KDLVKQFKRP LPEMLEDLQA MAAPMTALID LTKAFDTEFA RLKKEREMLD
FHDIERYAQA ILANDTIAVT LQKRYIQVFI DEYQDTNAVQ EAILSRVRRA DNYFMVGDVK
QSIYRFRQAD PTIFIEKYQR FQKQGRAADR LITLGQNFRS NQGIIDCVND VFSRIMSPEL
GEIAYDDEAA LICGLADSTH PAEPAELHVL QASASNHIEA EARYIAGRIH EMVGKEQWIA
KEGVSRPLRY RDIGVLMRSV SSKGETVARI LADAGIPTYF DAGASYYQSI EIIVFLNLIR
VIDNYQQDLP LLSVMRSPIG RFSDEELAAI RIGAKNRPFY QSLEAAAETK TALGQKARMF
LDHLERWRKF AYTQSVADFL WRLYLETGYY TAVGALPGGE QRQKNLRVLL QRASDYQTAT
LHGVFQFPDY AQPLTKRRQG DVMTPSILSE NDDVVRIMTI HKSKGLEFPV VFLADCGKGA
NLKNATGKVH FHQKLGICPK WIDPSAKLYH DTLATTLVQE QVKTESLSEE IRLLYVAMTR
AMAKLIMVGS VTDLEKEKER WEKPCMVGNL KKGRQGSGVS YLDWLMMACY RPGAAQNSVS
PALKIKIEEE TTREAVTQER PIQQLELSSK QLQEIKRRLS WHYQSAGRPA PSKLSVTQVE
HLRENPDRGA DALIEIPERM AKPAFMEAAG LRHWQPSELG SGIHTLLQRI DLDGIRACGG
DTAQIADAVR AEAARLAELG ILEAALVKAL DLRPVVRLMG SPIGKRLLQA VTCRRELPFN
LAVPGRRINP IYGEETVLLQ GMIDCCFIED DRWILLDYKT DRWHSQEARD RLIARYQIQI
DFYEQALAAI TGRPVAEKYL LFITMGEAVR LSPAEKTI
//
